Vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcohol
Background: Initiation of use/co-use of nicotine and alcohol, commonly occurring in an episodic manner during adolescence, can imprint vulnerability to the developing brain and lead to addiction. The ventral tegmental area (VTA) is a key heterogeneous region of the mesocorticolimbic circuit involved...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Drug and Alcohol Dependence Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772724623000501 |
| _version_ | 1797683020433457152 |
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| author | Maria Vrettou Stefan Bernhard Thalhammer Anne-Lie Svensson Sylvie Dumas Kent W Nilsson Åsa Wallén-Mackenzie Robert Fredriksson Ingrid Nylander Erika Comasco |
| author_facet | Maria Vrettou Stefan Bernhard Thalhammer Anne-Lie Svensson Sylvie Dumas Kent W Nilsson Åsa Wallén-Mackenzie Robert Fredriksson Ingrid Nylander Erika Comasco |
| author_sort | Maria Vrettou |
| collection | DOAJ |
| description | Background: Initiation of use/co-use of nicotine and alcohol, commonly occurring in an episodic manner during adolescence, can imprint vulnerability to the developing brain and lead to addiction. The ventral tegmental area (VTA) is a key heterogeneous region of the mesocorticolimbic circuit involved in the binge-drinking and intoxication step of the addiction circuit. Higher human post-mortem VTA expression of vesicular glutamate transporter 2 (VGLUT2), a marker of the glutamatergic phenotype also expressed in dopaminergic [Tyrosine Hydroxylase (Th)-positive] neurons, has been associated with chronic nicotine use and co-use with alcohol. Methods: The present study aimed to map and characterize the Vglut2- and Th-expressing neurons in the VTA of adolescent male rats exposed or not to prolonged (six-weeks) episodic (three consecutive days/week) nicotine and/or alcohol administration. Nicotine (0.35 mg/kg free base) was injected subcutaneously, whereas alcohol (2 g/kg 20%) was administrated via gavage. Vglut2 and Th mRNA was assessed in the anterior and posterior VTA by use of in situ hybridization. Results: The profile of neurons varied with substance-exposure among VTA subregions. Th-only expressing neurons were more abundant in the posterior VTA of the group exposed to nicotine-only, compared to controls. The same neurons were, on the contrary, less present in the anterior VTA of animals exposed to alcohol-only, who also displayed a higher number of Vglut2-expressing neurons in the lateral anterior VTA. Conclusions: VTA Vglut2- and Th-only neurons seem differentially involved in the effects of adolescent episodic nicotine and alcohol exposure in the anterior and posterior VTA. |
| first_indexed | 2024-03-12T00:08:23Z |
| format | Article |
| id | doaj.art-9b84faf55bfe4bb09339eedbc99cffb3 |
| institution | Directory Open Access Journal |
| issn | 2772-7246 |
| language | English |
| last_indexed | 2024-03-12T00:08:23Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Drug and Alcohol Dependence Reports |
| spelling | doaj.art-9b84faf55bfe4bb09339eedbc99cffb32023-09-16T05:32:03ZengElsevierDrug and Alcohol Dependence Reports2772-72462023-09-018100180Vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcoholMaria Vrettou0Stefan Bernhard Thalhammer1Anne-Lie Svensson2Sylvie Dumas3Kent W Nilsson4Åsa Wallén-Mackenzie5Robert Fredriksson6Ingrid Nylander7Erika Comasco8Department of Women's and Children's Health, Science for Life Laboratory, Uppsala University, Uppsala, SwedenDepartment of Women's and Children's Health, Science for Life Laboratory, Uppsala University, Uppsala, SwedenDepartment of Pharmaceutical Biosciences, Uppsala University, Uppsala, SwedenOramacell, Paris, FranceCentre for Clinical Research Västerås, Uppsala University, Västmanland County Hospital Västerås, SwedenDepartment of Organismal Biology, Uppsala University, Uppsala, SwedenDepartment of Pharmaceutical Biosciences, Uppsala University, Uppsala, SwedenDepartment of Pharmaceutical Biosciences, Uppsala University, Uppsala, SwedenDepartment of Women's and Children's Health, Science for Life Laboratory, Uppsala University, Uppsala, Sweden; Corresponding authors: Erika Comasco, Department of Women's and Children's Health, Science for Life Laboratory, Uppsala University, BMC, Box 593, 751 24 Uppsala, Sweden; telephone + 46 18 471 50 20Background: Initiation of use/co-use of nicotine and alcohol, commonly occurring in an episodic manner during adolescence, can imprint vulnerability to the developing brain and lead to addiction. The ventral tegmental area (VTA) is a key heterogeneous region of the mesocorticolimbic circuit involved in the binge-drinking and intoxication step of the addiction circuit. Higher human post-mortem VTA expression of vesicular glutamate transporter 2 (VGLUT2), a marker of the glutamatergic phenotype also expressed in dopaminergic [Tyrosine Hydroxylase (Th)-positive] neurons, has been associated with chronic nicotine use and co-use with alcohol. Methods: The present study aimed to map and characterize the Vglut2- and Th-expressing neurons in the VTA of adolescent male rats exposed or not to prolonged (six-weeks) episodic (three consecutive days/week) nicotine and/or alcohol administration. Nicotine (0.35 mg/kg free base) was injected subcutaneously, whereas alcohol (2 g/kg 20%) was administrated via gavage. Vglut2 and Th mRNA was assessed in the anterior and posterior VTA by use of in situ hybridization. Results: The profile of neurons varied with substance-exposure among VTA subregions. Th-only expressing neurons were more abundant in the posterior VTA of the group exposed to nicotine-only, compared to controls. The same neurons were, on the contrary, less present in the anterior VTA of animals exposed to alcohol-only, who also displayed a higher number of Vglut2-expressing neurons in the lateral anterior VTA. Conclusions: VTA Vglut2- and Th-only neurons seem differentially involved in the effects of adolescent episodic nicotine and alcohol exposure in the anterior and posterior VTA.http://www.sciencedirect.com/science/article/pii/S2772724623000501alcoholbrainexpressionnicotineVglut2 |
| spellingShingle | Maria Vrettou Stefan Bernhard Thalhammer Anne-Lie Svensson Sylvie Dumas Kent W Nilsson Åsa Wallén-Mackenzie Robert Fredriksson Ingrid Nylander Erika Comasco Vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcohol Drug and Alcohol Dependence Reports alcohol brain expression nicotine Vglut2 |
| title | Vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcohol |
| title_full | Vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcohol |
| title_fullStr | Vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcohol |
| title_full_unstemmed | Vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcohol |
| title_short | Vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcohol |
| title_sort | vesicular glutamate transporter 2 expression in the ventral tegmental area of outbred male rats following exposure to nicotine and alcohol |
| topic | alcohol brain expression nicotine Vglut2 |
| url | http://www.sciencedirect.com/science/article/pii/S2772724623000501 |
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