MONOCYTES OF HUMAN BLOOD AS A TARGET OF STREPTOCOCCUS PYOGENES COMPONENTS
Aim. Study the effect of components of destroyed streptococci on human blood monocyte functions related to processes of trans-endothelial migration in vitro. Materials and methods. Mononuclear leukocytes, isolated from blood of healthy donors, endothelial cells of EA.hy 926 line and supernatant of u...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2015-02-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.crie.ru/jour/article/view/14187 |
| _version_ | 1827903081950478336 |
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| author | A. M Lebedeva E. A Starikova L. A Burova I. S Freidlin K. A Samoilova |
| author_facet | A. M Lebedeva E. A Starikova L. A Burova I. S Freidlin K. A Samoilova |
| author_sort | A. M Lebedeva |
| collection | DOAJ |
| description | Aim. Study the effect of components of destroyed streptococci on human blood monocyte functions related to processes of trans-endothelial migration in vitro. Materials and methods. Mononuclear leukocytes, isolated from blood of healthy donors, endothelial cells of EA.hy 926 line and supernatant of ultrasound disintegrated Streptococcus pyogenes (DSS) were the objects of the study. Evaluation of adhesion and monocyte migration, level of expression of adhesion molecules and phosphokinases on monocytes was carried out by flow cytometry using monoclonal antibodies. Cytokine concentration was determined by using standard commercial test systems in enzyme immunoassay. Results. Under the effect of DSS, expression of adhesion molecules CD162 and CD11b, as well as phospho-p38 MAPK changed, IL-6 and IL-8 secretion induction took place. DSS caused enhancement of migration and adhesive activity of monocytes, however, inhibited intensity of trans-endothelial migration. Conclusion. Products of destroyed streptococci have a multi-directional effect on human blood monocytes, that could be explained by the presence of components with varying biological activity in DSS. |
| first_indexed | 2024-03-13T00:07:56Z |
| format | Article |
| id | doaj.art-9b85a7f619cd424f9680859f2772248b |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-13T00:07:56Z |
| publishDate | 2015-02-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-9b85a7f619cd424f9680859f2772248b2023-07-12T20:21:07ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132015-02-0192139452611MONOCYTES OF HUMAN BLOOD AS A TARGET OF STREPTOCOCCUS PYOGENES COMPONENTSA. M Lebedeva0E. A Starikova1L. A Burova2I. S Freidlin3K. A Samoilova4Research Institute of Experimental MedicineResearch Institute of Experimental MedicineResearch Institute of Experimental MedicineResearch Institute of Experimental MedicineInstitute of CytologyAim. Study the effect of components of destroyed streptococci on human blood monocyte functions related to processes of trans-endothelial migration in vitro. Materials and methods. Mononuclear leukocytes, isolated from blood of healthy donors, endothelial cells of EA.hy 926 line and supernatant of ultrasound disintegrated Streptococcus pyogenes (DSS) were the objects of the study. Evaluation of adhesion and monocyte migration, level of expression of adhesion molecules and phosphokinases on monocytes was carried out by flow cytometry using monoclonal antibodies. Cytokine concentration was determined by using standard commercial test systems in enzyme immunoassay. Results. Under the effect of DSS, expression of adhesion molecules CD162 and CD11b, as well as phospho-p38 MAPK changed, IL-6 and IL-8 secretion induction took place. DSS caused enhancement of migration and adhesive activity of monocytes, however, inhibited intensity of trans-endothelial migration. Conclusion. Products of destroyed streptococci have a multi-directional effect on human blood monocytes, that could be explained by the presence of components with varying biological activity in DSS.https://microbiol.crie.ru/jour/article/view/14187s. pyogenesmonocytesendothelial cellss. pyogenesadhesiontransmigration |
| spellingShingle | A. M Lebedeva E. A Starikova L. A Burova I. S Freidlin K. A Samoilova MONOCYTES OF HUMAN BLOOD AS A TARGET OF STREPTOCOCCUS PYOGENES COMPONENTS Журнал микробиологии, эпидемиологии и иммунобиологии s. pyogenes monocytes endothelial cells s. pyogenes adhesion transmigration |
| title | MONOCYTES OF HUMAN BLOOD AS A TARGET OF STREPTOCOCCUS PYOGENES COMPONENTS |
| title_full | MONOCYTES OF HUMAN BLOOD AS A TARGET OF STREPTOCOCCUS PYOGENES COMPONENTS |
| title_fullStr | MONOCYTES OF HUMAN BLOOD AS A TARGET OF STREPTOCOCCUS PYOGENES COMPONENTS |
| title_full_unstemmed | MONOCYTES OF HUMAN BLOOD AS A TARGET OF STREPTOCOCCUS PYOGENES COMPONENTS |
| title_short | MONOCYTES OF HUMAN BLOOD AS A TARGET OF STREPTOCOCCUS PYOGENES COMPONENTS |
| title_sort | monocytes of human blood as a target of streptococcus pyogenes components |
| topic | s. pyogenes monocytes endothelial cells s. pyogenes adhesion transmigration |
| url | https://microbiol.crie.ru/jour/article/view/14187 |
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