The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer
Stem properties allow circulating tumor cells (CTCs) to survive in the bloodstream and initiate cancer progression. We aimed to assess the numbers of stem-like CTCs in patients with ovarian cancer (OC) before treatment and during first-line chemotherapy (CT). Flow cytometry was performed (Cytoflex S...
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2021-08-01
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| author | Snezhanna O. Gening Tatyana V. Abakumova Dina U. Gafurbaeva Albert A. Rizvanov Inna I. Antoneeva Regina R. Miftakhova Andrey B. Peskov Tatyana P. Gening |
| author_facet | Snezhanna O. Gening Tatyana V. Abakumova Dina U. Gafurbaeva Albert A. Rizvanov Inna I. Antoneeva Regina R. Miftakhova Andrey B. Peskov Tatyana P. Gening |
| author_sort | Snezhanna O. Gening |
| collection | DOAJ |
| description | Stem properties allow circulating tumor cells (CTCs) to survive in the bloodstream and initiate cancer progression. We aimed to assess the numbers of stem-like CTCs in patients with ovarian cancer (OC) before treatment and during first-line chemotherapy (CT). Flow cytometry was performed (Cytoflex S (Beckman Coulter, CA, USA)) using antibodies against CD45; epithelial markers EpCAM and cytokeratin (CK) 8,18; mesenchymal vimentin (vim); and stem-like CD44, CD133 and ALDH. This study included 38 stage I–IV OC patients (median age 66 (Q1–Q3 53–70)). The CK+vim- counts were higher (<i>p</i> = 0.012) and the CD133+ALDHhigh counts were lower (<i>p</i> = 0.010) before treatment in the neoadjuvant CT group than in the adjuvant group. The patients with ascites had more CK+vim- cells before treatment (<i>p</i> = 0.009) and less EpCAM-vim+ cells during treatment (<i>p</i> = 0.018) than the patients without ascites. All the CTC counts did not differ significantly in paired samples. Correlations were found between the CK-vim+ and CD133+ALDHhigh (r = 0.505, <i>p</i> = 0.027) and EpCAM-vim+ and ALDHhigh (r = 0.597, <i>p</i> = 0.004) cells before but not during treatment. Multivariate Cox regression analysis showed that progression-free survival was longer with the presence of surgical treatment (HR 0.06 95% CI 0.01–0.48, <i>p</i> = 0.009) and fewer CD133+ALDHveryhigh cells (HR 1.06 95% CI 1.02–1.12, <i>p</i> = 0.010). Thus, CD133+ALDH+ CTCs have the greatest prognostic potential in OC among the phenotypes studied. |
| first_indexed | 2024-03-10T08:40:12Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T08:40:12Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-9b8a968b1db140449ec7be8acfcf41f32023-11-22T08:23:18ZengMDPI AGLife2075-17292021-08-0111881510.3390/life11080815The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian CancerSnezhanna O. Gening0Tatyana V. Abakumova1Dina U. Gafurbaeva2Albert A. Rizvanov3Inna I. Antoneeva4Regina R. Miftakhova5Andrey B. Peskov6Tatyana P. Gening7Department of Human Physiology and Pathophysiology, Ulyanovsk State University, 42 Leo Tolstoy St., 432017 Ulyanovsk, RussiaDepartment of Human Physiology and Pathophysiology, Ulyanovsk State University, 42 Leo Tolstoy St., 432017 Ulyanovsk, RussiaOpenLab ‘Gene and Cell Technologies’, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaOpenLab ‘Gene and Cell Technologies’, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaUlyanovsk Regional Clinical Oncology Center, 90 12th September St., 432048 Ulyanovsk, RussiaOpenLab ‘Gene and Cell Technologies’, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaDepartment of Human Physiology and Pathophysiology, Ulyanovsk State University, 42 Leo Tolstoy St., 432017 Ulyanovsk, RussiaDepartment of Human Physiology and Pathophysiology, Ulyanovsk State University, 42 Leo Tolstoy St., 432017 Ulyanovsk, RussiaStem properties allow circulating tumor cells (CTCs) to survive in the bloodstream and initiate cancer progression. We aimed to assess the numbers of stem-like CTCs in patients with ovarian cancer (OC) before treatment and during first-line chemotherapy (CT). Flow cytometry was performed (Cytoflex S (Beckman Coulter, CA, USA)) using antibodies against CD45; epithelial markers EpCAM and cytokeratin (CK) 8,18; mesenchymal vimentin (vim); and stem-like CD44, CD133 and ALDH. This study included 38 stage I–IV OC patients (median age 66 (Q1–Q3 53–70)). The CK+vim- counts were higher (<i>p</i> = 0.012) and the CD133+ALDHhigh counts were lower (<i>p</i> = 0.010) before treatment in the neoadjuvant CT group than in the adjuvant group. The patients with ascites had more CK+vim- cells before treatment (<i>p</i> = 0.009) and less EpCAM-vim+ cells during treatment (<i>p</i> = 0.018) than the patients without ascites. All the CTC counts did not differ significantly in paired samples. Correlations were found between the CK-vim+ and CD133+ALDHhigh (r = 0.505, <i>p</i> = 0.027) and EpCAM-vim+ and ALDHhigh (r = 0.597, <i>p</i> = 0.004) cells before but not during treatment. Multivariate Cox regression analysis showed that progression-free survival was longer with the presence of surgical treatment (HR 0.06 95% CI 0.01–0.48, <i>p</i> = 0.009) and fewer CD133+ALDHveryhigh cells (HR 1.06 95% CI 1.02–1.12, <i>p</i> = 0.010). Thus, CD133+ALDH+ CTCs have the greatest prognostic potential in OC among the phenotypes studied.https://www.mdpi.com/2075-1729/11/8/815ovarian cancerstem cellscirculating tumor cellsCTCsCD44CD133 |
| spellingShingle | Snezhanna O. Gening Tatyana V. Abakumova Dina U. Gafurbaeva Albert A. Rizvanov Inna I. Antoneeva Regina R. Miftakhova Andrey B. Peskov Tatyana P. Gening The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer Life ovarian cancer stem cells circulating tumor cells CTCs CD44 CD133 |
| title | The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer |
| title_full | The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer |
| title_fullStr | The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer |
| title_full_unstemmed | The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer |
| title_short | The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer |
| title_sort | detection of stem like circulating tumor cells could increase the clinical applicability of liquid biopsy in ovarian cancer |
| topic | ovarian cancer stem cells circulating tumor cells CTCs CD44 CD133 |
| url | https://www.mdpi.com/2075-1729/11/8/815 |
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