Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium
Nowadays, the bone osseointegration in different environments is comparable, but the mechanism is unclear. This study aimed to investigate the osseointegration of different bioactive titanium surfaces under normoxic or high-altitude hypoxic environments. Titanium implants were subjected to one of tw...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.689807/full |
| _version_ | 1819175761714610176 |
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| author | Yarong Wang Zekun Gan Haibin Lu Ziyi Liu Peng Shang Jian Zhang Wuwei Yin Hongxing Chu Renlei Yuan Yingxin Ye Pei Chen Mingdeng Rong |
| author_facet | Yarong Wang Zekun Gan Haibin Lu Ziyi Liu Peng Shang Jian Zhang Wuwei Yin Hongxing Chu Renlei Yuan Yingxin Ye Pei Chen Mingdeng Rong |
| author_sort | Yarong Wang |
| collection | DOAJ |
| description | Nowadays, the bone osseointegration in different environments is comparable, but the mechanism is unclear. This study aimed to investigate the osseointegration of different bioactive titanium surfaces under normoxic or high-altitude hypoxic environments. Titanium implants were subjected to one of two surface treatments: (1) sanding, blasting, and acid etching to obtain a rough surface, or (2) extensive polishing to obtain a smooth surface. Changes in the morphology, proliferation, and protein expression of osteoblasts on the rough and smooth surfaces were examined, and bone formation was studied through western blotting and animal-based experiments. Our findings found that a hypoxic environment and rough titanium implant surface promoted the osteogenic differentiation of osteoblasts and activated the JAK1/STAT1/HIF-1α pathway in vitro. The animal study revealed that following implant insertion in tibia of rabbit, bone repair at high altitudes was slower than that at low altitudes (i.e., in plains) after 2weeks; however, bone formation did not differ significantly after 4weeks. The results of our study showed that: (1) The altitude hypoxia environment would affect the early osseointegration of titanium implants while titanium implants with rough surfaces can mitigate the effects of this hypoxic environment on osseointegration, (2) the mechanism may be related to the activation of JAK1/STAT1/HIF-1α pathway, and (3) our results suggest the osteogenesis of titanium implants, such as oral implants, is closely related to the oxygen environment. Clinical doctors, especially dentists, should pay attention to the influence of hypoxia on early osseointegration in patients with high altitude. For example, it is better to choose an implant system with rough implant surface in the oral cavity of patients with tooth loss at high altitude. |
| first_indexed | 2024-12-22T21:00:00Z |
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| id | doaj.art-9b8b255029684b5babc18f21f9c6c7fe |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-22T21:00:00Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-9b8b255029684b5babc18f21f9c6c7fe2022-12-21T18:12:51ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-11-011210.3389/fphys.2021.689807689807Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive TitaniumYarong Wang0Zekun Gan1Haibin Lu2Ziyi Liu3Peng Shang4Jian Zhang5Wuwei Yin6Hongxing Chu7Renlei Yuan8Yingxin Ye9Pei Chen10Mingdeng Rong11Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaDepartment of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaLinzhi People’s Hospital, Linzhi, ChinaDepartment of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, ChinaNowadays, the bone osseointegration in different environments is comparable, but the mechanism is unclear. This study aimed to investigate the osseointegration of different bioactive titanium surfaces under normoxic or high-altitude hypoxic environments. Titanium implants were subjected to one of two surface treatments: (1) sanding, blasting, and acid etching to obtain a rough surface, or (2) extensive polishing to obtain a smooth surface. Changes in the morphology, proliferation, and protein expression of osteoblasts on the rough and smooth surfaces were examined, and bone formation was studied through western blotting and animal-based experiments. Our findings found that a hypoxic environment and rough titanium implant surface promoted the osteogenic differentiation of osteoblasts and activated the JAK1/STAT1/HIF-1α pathway in vitro. The animal study revealed that following implant insertion in tibia of rabbit, bone repair at high altitudes was slower than that at low altitudes (i.e., in plains) after 2weeks; however, bone formation did not differ significantly after 4weeks. The results of our study showed that: (1) The altitude hypoxia environment would affect the early osseointegration of titanium implants while titanium implants with rough surfaces can mitigate the effects of this hypoxic environment on osseointegration, (2) the mechanism may be related to the activation of JAK1/STAT1/HIF-1α pathway, and (3) our results suggest the osteogenesis of titanium implants, such as oral implants, is closely related to the oxygen environment. Clinical doctors, especially dentists, should pay attention to the influence of hypoxia on early osseointegration in patients with high altitude. For example, it is better to choose an implant system with rough implant surface in the oral cavity of patients with tooth loss at high altitude.https://www.frontiersin.org/articles/10.3389/fphys.2021.689807/fullosseointegrationbioactive titaniumhypoxianormoxiaosteoblast |
| spellingShingle | Yarong Wang Zekun Gan Haibin Lu Ziyi Liu Peng Shang Jian Zhang Wuwei Yin Hongxing Chu Renlei Yuan Yingxin Ye Pei Chen Mingdeng Rong Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium Frontiers in Physiology osseointegration bioactive titanium hypoxia normoxia osteoblast |
| title | Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium |
| title_full | Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium |
| title_fullStr | Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium |
| title_full_unstemmed | Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium |
| title_short | Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium |
| title_sort | impact of high altitude hypoxia on early osseointegration with bioactive titanium |
| topic | osseointegration bioactive titanium hypoxia normoxia osteoblast |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.689807/full |
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