Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum

Abstract Defense responses of tomato (Solanum lycopersicum L.) against attack by Pectobacterium carotovorum subsp. carotovorum (Pcc), the causal agent of soft rot diseases, were studied. The expression of some tomato defense genes were evaluated by real‐time PCR quantification analysis, 24 and 72 hr...

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Main Authors: Arnaud T. Djami‐Tchatchou, Lerato B. T. Matsaunyane, Khayalethu Ntushelo
Format: Article
Language:English
Published: Wiley 2019-12-01
Series:MicrobiologyOpen
Subjects:
Online Access:https://doi.org/10.1002/mbo3.911
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author Arnaud T. Djami‐Tchatchou
Lerato B. T. Matsaunyane
Khayalethu Ntushelo
author_facet Arnaud T. Djami‐Tchatchou
Lerato B. T. Matsaunyane
Khayalethu Ntushelo
author_sort Arnaud T. Djami‐Tchatchou
collection DOAJ
description Abstract Defense responses of tomato (Solanum lycopersicum L.) against attack by Pectobacterium carotovorum subsp. carotovorum (Pcc), the causal agent of soft rot diseases, were studied. The expression of some tomato defense genes were evaluated by real‐time PCR quantification analysis, 24 and 72 hr after actively growing tomato plants were inoculated with Pcc. These included: MYB transcriptor factor, ethylene response element‐binding protein, suppressor of the G2 allele of Skp1, cytochrome P450, small Sar1 GTPase, hydroxycinnamoyl‐CoA:quinate hydroxycinnamoyl transferase, pathogenesis‐related protein 1a, endo‐1,3‐beta‐glucanase, chitinase, proteinase inhibitor, defensin, CC‐NBS‐LRR resistance protein, and phenylalanine ammonia lyase. The results showed dynamic transcriptomic changes, with transcripts exhibiting different expression kinetics at 24 and 72 hr to confer resistance to tomato against Pcc infection.
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spelling doaj.art-9b94933cb04c4adb964094488c9eb5cc2022-12-21T19:46:23ZengWileyMicrobiologyOpen2045-88272019-12-01812n/an/a10.1002/mbo3.911Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorumArnaud T. Djami‐Tchatchou0Lerato B. T. Matsaunyane1Khayalethu Ntushelo2Department of Agriculture and Animal Health, Science Campus University of South Africa Florida South AfricaAgricultural Research Council‐Vegetable and Ornamental Plants Tshwane South AfricaDepartment of Agriculture and Animal Health, Science Campus University of South Africa Florida South AfricaAbstract Defense responses of tomato (Solanum lycopersicum L.) against attack by Pectobacterium carotovorum subsp. carotovorum (Pcc), the causal agent of soft rot diseases, were studied. The expression of some tomato defense genes were evaluated by real‐time PCR quantification analysis, 24 and 72 hr after actively growing tomato plants were inoculated with Pcc. These included: MYB transcriptor factor, ethylene response element‐binding protein, suppressor of the G2 allele of Skp1, cytochrome P450, small Sar1 GTPase, hydroxycinnamoyl‐CoA:quinate hydroxycinnamoyl transferase, pathogenesis‐related protein 1a, endo‐1,3‐beta‐glucanase, chitinase, proteinase inhibitor, defensin, CC‐NBS‐LRR resistance protein, and phenylalanine ammonia lyase. The results showed dynamic transcriptomic changes, with transcripts exhibiting different expression kinetics at 24 and 72 hr to confer resistance to tomato against Pcc infection.https://doi.org/10.1002/mbo3.911defensegene expressionPectobacterium carotovorum subsp. carotovorumtomato
spellingShingle Arnaud T. Djami‐Tchatchou
Lerato B. T. Matsaunyane
Khayalethu Ntushelo
Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum
MicrobiologyOpen
defense
gene expression
Pectobacterium carotovorum subsp. carotovorum
tomato
title Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum
title_full Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum
title_fullStr Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum
title_full_unstemmed Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum
title_short Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum
title_sort gene expression responses of tomato inoculated with pectobacterium carotovorum subsp carotovorum
topic defense
gene expression
Pectobacterium carotovorum subsp. carotovorum
tomato
url https://doi.org/10.1002/mbo3.911
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AT khayalethuntushelo geneexpressionresponsesoftomatoinoculatedwithpectobacteriumcarotovorumsubspcarotovorum