Profiles of Metabolic Genes in <i>Uncaria rhynchophylla</i> and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline
Rhynchophylline (RIN) and isorhynchophylline (IRN), two of the representative types of indole alkaloids, showed the unique spiroindole structures produced in <i>Uncaria rhynchophylla</i>. As the bioactive constituent of <i>U. rhynchophylla</i>, IRN has recently drawn extensiv...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/12/1790 |
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| author | Mengquan Yang Bowen Yao Rongmei Lin |
| author_facet | Mengquan Yang Bowen Yao Rongmei Lin |
| author_sort | Mengquan Yang |
| collection | DOAJ |
| description | Rhynchophylline (RIN) and isorhynchophylline (IRN), two of the representative types of indole alkaloids, showed the unique spiroindole structures produced in <i>Uncaria rhynchophylla</i>. As the bioactive constituent of <i>U. rhynchophylla</i>, IRN has recently drawn extensive attention toward antihypertensive and neuroprotective activities. Despite their medicinal importance and unique chemical structure, the biosynthetic pathways of plant spiroindole alkaloids are still largely unknown. In this study, we used <i>U. rhynchophylla</i>, extensively used in traditional Chinese medicine (TCM), a widely cultivated plant of the <i>Uncaria</i> genus, to investigate the biosynthetic genes and characterize the functional enzymes in the spiroindole alkaloids. We aim to use the transcriptome platform to analyse the tissue-specific gene expression in spiroindole alkaloids-producing tissues, including root, bud, stem bark and leaf. The critical genes involved in the biosynthesis of precursors and scaffold formation of spiroindole alkaloids were discovered and characterized. The datasets from this work provide an essential resource for further investigating metabolic pathways in <i>U. rhynchophylla</i> and facilitate novel functional enzyme characterization and a good biopharming approach to spiroindole alkaloids. |
| first_indexed | 2024-03-09T17:17:27Z |
| format | Article |
| id | doaj.art-9b98b54df3874630b7987cb897e00677 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T17:17:27Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9b98b54df3874630b7987cb897e006772023-11-24T13:33:22ZengMDPI AGBiomolecules2218-273X2022-11-011212179010.3390/biom12121790Profiles of Metabolic Genes in <i>Uncaria rhynchophylla</i> and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)RhynchophyllineMengquan Yang0Bowen Yao1Rongmei Lin2College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaSchool of Science, Beijing University of Chemical Technology, Chaoyang District, Beijing 100029, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaRhynchophylline (RIN) and isorhynchophylline (IRN), two of the representative types of indole alkaloids, showed the unique spiroindole structures produced in <i>Uncaria rhynchophylla</i>. As the bioactive constituent of <i>U. rhynchophylla</i>, IRN has recently drawn extensive attention toward antihypertensive and neuroprotective activities. Despite their medicinal importance and unique chemical structure, the biosynthetic pathways of plant spiroindole alkaloids are still largely unknown. In this study, we used <i>U. rhynchophylla</i>, extensively used in traditional Chinese medicine (TCM), a widely cultivated plant of the <i>Uncaria</i> genus, to investigate the biosynthetic genes and characterize the functional enzymes in the spiroindole alkaloids. We aim to use the transcriptome platform to analyse the tissue-specific gene expression in spiroindole alkaloids-producing tissues, including root, bud, stem bark and leaf. The critical genes involved in the biosynthesis of precursors and scaffold formation of spiroindole alkaloids were discovered and characterized. The datasets from this work provide an essential resource for further investigating metabolic pathways in <i>U. rhynchophylla</i> and facilitate novel functional enzyme characterization and a good biopharming approach to spiroindole alkaloids.https://www.mdpi.com/2218-273X/12/12/1790<i>Uncaria rhynchophylla</i>spiroindole alkaloidsbiosynthetic pathwaytranscriptomerhynchophylline and isorhynchophylline |
| spellingShingle | Mengquan Yang Bowen Yao Rongmei Lin Profiles of Metabolic Genes in <i>Uncaria rhynchophylla</i> and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline Biomolecules <i>Uncaria rhynchophylla</i> spiroindole alkaloids biosynthetic pathway transcriptome rhynchophylline and isorhynchophylline |
| title | Profiles of Metabolic Genes in <i>Uncaria rhynchophylla</i> and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline |
| title_full | Profiles of Metabolic Genes in <i>Uncaria rhynchophylla</i> and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline |
| title_fullStr | Profiles of Metabolic Genes in <i>Uncaria rhynchophylla</i> and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline |
| title_full_unstemmed | Profiles of Metabolic Genes in <i>Uncaria rhynchophylla</i> and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline |
| title_short | Profiles of Metabolic Genes in <i>Uncaria rhynchophylla</i> and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline |
| title_sort | profiles of metabolic genes in i uncaria rhynchophylla i and characterization of the critical enzyme involved in the biosynthesis of bioactive compounds iso rhynchophylline |
| topic | <i>Uncaria rhynchophylla</i> spiroindole alkaloids biosynthetic pathway transcriptome rhynchophylline and isorhynchophylline |
| url | https://www.mdpi.com/2218-273X/12/12/1790 |
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