Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation
This study aimed to characterize raw honeys from different geographical origins in Romania, in respect of chemical composition, microbiological examination and evaluate their probiotic potential. The physico-chemical determinations were performed in APHIS-DIA Laboratory, Cluj-Napoca, Romania, using...
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MDPI AG
2021-06-01
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| author | Claudia Pașca Liviu Alexandru Mărghitaș Ioana Adriana Matei Victorița Bonta Rodica Mărgăoan Florina Copaciu Otilia Bobiș Maria Graça Campos Daniel Severus Dezmirean |
| author_facet | Claudia Pașca Liviu Alexandru Mărghitaș Ioana Adriana Matei Victorița Bonta Rodica Mărgăoan Florina Copaciu Otilia Bobiș Maria Graça Campos Daniel Severus Dezmirean |
| author_sort | Claudia Pașca |
| collection | DOAJ |
| description | This study aimed to characterize raw honeys from different geographical origins in Romania, in respect of chemical composition, microbiological examination and evaluate their probiotic potential. The physico-chemical determinations were performed in APHIS-DIA Laboratory, Cluj-Napoca, Romania, using standard validated methods. Bacterial identification was performed for each sample and each colony type using Vitek<sup>®</sup> 2 Compact 15 system and PCR amplification using 16S rDNA bacterial universal primers (27F, 1492R), species being confirm by sequences analysis. In five raw honey samples, we have identified probiotic bacteria, such as: <i>Bacillus mycoides</i>, <i>Bacillus thuringiensis</i>, <i>Bacillus amyloliquefaciens</i>, <i>Bacillus subtilis</i>, and <i>Bacillus velezensis.</i> Generally, all honey samples meet the standard values for chemical composition. However, one sample having 7.44% sucrose was found to have also probiotics bacteria from the genus Bacillus because sucrose is a substrate for probiotics development. In conclusion, the Romanian raw honey can be a potential reservoir of probiotics, which confer a health benefit for consumers. |
| first_indexed | 2024-03-10T10:07:42Z |
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| id | doaj.art-9b98d10ee0764092b49076c1d707d429 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T10:07:42Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-9b98d10ee0764092b49076c1d707d4292023-11-22T01:24:17ZengMDPI AGApplied Sciences2076-34172021-06-011113581610.3390/app11135816Screening of Some Romanian Raw Honeys and Their Probiotic Potential EvaluationClaudia Pașca0Liviu Alexandru Mărghitaș1Ioana Adriana Matei2Victorița Bonta3Rodica Mărgăoan4Florina Copaciu5Otilia Bobiș6Maria Graça Campos7Daniel Severus Dezmirean8Department of Apiculture and Sericulture, Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Apiculture and Sericulture, Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Microbiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Apiculture and Sericulture, Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaAdvanced Horticultural Research Institute of Transylvania, Faculty of Horticulture, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, RomaniaDepartment of Chemistry and Biochemistry, Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Apiculture and Sericulture, Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaCQ-Centre of Chemistry—Coimbra, Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, Rua Larga, 3004-535 Coimbra, PortugalDepartment of Apiculture and Sericulture, Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaThis study aimed to characterize raw honeys from different geographical origins in Romania, in respect of chemical composition, microbiological examination and evaluate their probiotic potential. The physico-chemical determinations were performed in APHIS-DIA Laboratory, Cluj-Napoca, Romania, using standard validated methods. Bacterial identification was performed for each sample and each colony type using Vitek<sup>®</sup> 2 Compact 15 system and PCR amplification using 16S rDNA bacterial universal primers (27F, 1492R), species being confirm by sequences analysis. In five raw honey samples, we have identified probiotic bacteria, such as: <i>Bacillus mycoides</i>, <i>Bacillus thuringiensis</i>, <i>Bacillus amyloliquefaciens</i>, <i>Bacillus subtilis</i>, and <i>Bacillus velezensis.</i> Generally, all honey samples meet the standard values for chemical composition. However, one sample having 7.44% sucrose was found to have also probiotics bacteria from the genus Bacillus because sucrose is a substrate for probiotics development. In conclusion, the Romanian raw honey can be a potential reservoir of probiotics, which confer a health benefit for consumers.https://www.mdpi.com/2076-3417/11/13/5816honeycombsfructooligosaccharides from honeyprobiotic <i>Bacillus</i> strainsphysicochemical characterizationantimicrobial activity |
| spellingShingle | Claudia Pașca Liviu Alexandru Mărghitaș Ioana Adriana Matei Victorița Bonta Rodica Mărgăoan Florina Copaciu Otilia Bobiș Maria Graça Campos Daniel Severus Dezmirean Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation Applied Sciences honeycombs fructooligosaccharides from honey probiotic <i>Bacillus</i> strains physicochemical characterization antimicrobial activity |
| title | Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation |
| title_full | Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation |
| title_fullStr | Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation |
| title_full_unstemmed | Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation |
| title_short | Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation |
| title_sort | screening of some romanian raw honeys and their probiotic potential evaluation |
| topic | honeycombs fructooligosaccharides from honey probiotic <i>Bacillus</i> strains physicochemical characterization antimicrobial activity |
| url | https://www.mdpi.com/2076-3417/11/13/5816 |
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