<i>LINE-1</i> Methylation Status in Canine Splenic Hemangiosarcoma Tissue and Cell-Free DNA
Splenic hemangiosarcoma is one of the most common malignant tumors in dogs, and early diagnosis is of great importance for achieving a good prognosis. DNA methylation plays an important role in cancer development. Long interspersed nuclear element 1 (<i>LINE-1</i>) is the most abundant r...
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MDPI AG
2023-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/18/2987 |
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| author | Hiroki Sato Ken-Ichi Watanabe Yoshiyasu Kobayashi Mizuki Tomihari Akiko Uemura Michihito Tagawa |
| author_facet | Hiroki Sato Ken-Ichi Watanabe Yoshiyasu Kobayashi Mizuki Tomihari Akiko Uemura Michihito Tagawa |
| author_sort | Hiroki Sato |
| collection | DOAJ |
| description | Splenic hemangiosarcoma is one of the most common malignant tumors in dogs, and early diagnosis is of great importance for achieving a good prognosis. DNA methylation plays an important role in cancer development. Long interspersed nuclear element 1 (<i>LINE-1</i>) is the most abundant repetitive element in the genome. <i>LINE-1</i> hypomethylation has been shown to be related to carcinogenesis in humans, and it has been used as a novel cancer biomarker. This study aimed to evaluate the methylation status of <i>LINE-1</i> in tumor tissue and circulating cell-free DNA and assess its clinical significance in canine splenic hemangiosarcoma. Genomic DNA was isolated from splenic masses of 13 dogs with hemangiosarcoma, 11 with other malignant tumors, and 15 with benign lesions. <i>LINE-1</i> methylation was quantified using methylation-sensitive and -insensitive restriction enzyme digestion followed by real-time polymerase chain reaction. Additionally, blood samples were collected from eight patients to isolate cell-free DNA to determine <i>LINE-1</i> methylation status changes during the treatment course. <i>LINE-1</i> methylation in tumor samples was significantly lower in patients with hemangiosarcoma than in those with other malignant tumors and benign lesions. Non-significant but similar results were observed for the cell-free DNA samples. Our results demonstrate that <i>LINE-1</i> methylation status is a potential biomarker for splenic hemangiosarcoma. |
| first_indexed | 2024-03-10T23:06:40Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T23:06:40Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-9b99c2a60e334e8eaed0f06902dc5d282023-11-19T09:16:23ZengMDPI AGAnimals2076-26152023-09-011318298710.3390/ani13182987<i>LINE-1</i> Methylation Status in Canine Splenic Hemangiosarcoma Tissue and Cell-Free DNAHiroki Sato0Ken-Ichi Watanabe1Yoshiyasu Kobayashi2Mizuki Tomihari3Akiko Uemura4Michihito Tagawa5Veterinary Medical Center, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanResearch Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanResearch Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanDepartment of Veterinary Science, Osaka Metropolitan University, Izumisano 545-8585, JapanDepartment of Veterinary Clinical Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanVeterinary Medical Center, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanSplenic hemangiosarcoma is one of the most common malignant tumors in dogs, and early diagnosis is of great importance for achieving a good prognosis. DNA methylation plays an important role in cancer development. Long interspersed nuclear element 1 (<i>LINE-1</i>) is the most abundant repetitive element in the genome. <i>LINE-1</i> hypomethylation has been shown to be related to carcinogenesis in humans, and it has been used as a novel cancer biomarker. This study aimed to evaluate the methylation status of <i>LINE-1</i> in tumor tissue and circulating cell-free DNA and assess its clinical significance in canine splenic hemangiosarcoma. Genomic DNA was isolated from splenic masses of 13 dogs with hemangiosarcoma, 11 with other malignant tumors, and 15 with benign lesions. <i>LINE-1</i> methylation was quantified using methylation-sensitive and -insensitive restriction enzyme digestion followed by real-time polymerase chain reaction. Additionally, blood samples were collected from eight patients to isolate cell-free DNA to determine <i>LINE-1</i> methylation status changes during the treatment course. <i>LINE-1</i> methylation in tumor samples was significantly lower in patients with hemangiosarcoma than in those with other malignant tumors and benign lesions. Non-significant but similar results were observed for the cell-free DNA samples. Our results demonstrate that <i>LINE-1</i> methylation status is a potential biomarker for splenic hemangiosarcoma.https://www.mdpi.com/2076-2615/13/18/2987dog<i>LINE-1</i>methylationhemangiosarcomacell-free DNAliquid biopsy |
| spellingShingle | Hiroki Sato Ken-Ichi Watanabe Yoshiyasu Kobayashi Mizuki Tomihari Akiko Uemura Michihito Tagawa <i>LINE-1</i> Methylation Status in Canine Splenic Hemangiosarcoma Tissue and Cell-Free DNA Animals dog <i>LINE-1</i> methylation hemangiosarcoma cell-free DNA liquid biopsy |
| title | <i>LINE-1</i> Methylation Status in Canine Splenic Hemangiosarcoma Tissue and Cell-Free DNA |
| title_full | <i>LINE-1</i> Methylation Status in Canine Splenic Hemangiosarcoma Tissue and Cell-Free DNA |
| title_fullStr | <i>LINE-1</i> Methylation Status in Canine Splenic Hemangiosarcoma Tissue and Cell-Free DNA |
| title_full_unstemmed | <i>LINE-1</i> Methylation Status in Canine Splenic Hemangiosarcoma Tissue and Cell-Free DNA |
| title_short | <i>LINE-1</i> Methylation Status in Canine Splenic Hemangiosarcoma Tissue and Cell-Free DNA |
| title_sort | i line 1 i methylation status in canine splenic hemangiosarcoma tissue and cell free dna |
| topic | dog <i>LINE-1</i> methylation hemangiosarcoma cell-free DNA liquid biopsy |
| url | https://www.mdpi.com/2076-2615/13/18/2987 |
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