Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces
Septoria tritici blotch (STB) is a devastating fungal disease affecting durum and bread wheat cultivation worldwide. The identification, development, and employment of resistant wheat genetic material is the key to overcoming costs and limitations of fungicide treatments. The search for resistance s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01586/full |
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| author | Yosef G. Kidane Yosef G. Kidane Yosef G. Kidane Bogale N. Hailemariam Dejene K. Mengistu Dejene K. Mengistu Carlo Fadda Mario Enrico Pè Matteo Dell'Acqua |
| author_facet | Yosef G. Kidane Yosef G. Kidane Yosef G. Kidane Bogale N. Hailemariam Dejene K. Mengistu Dejene K. Mengistu Carlo Fadda Mario Enrico Pè Matteo Dell'Acqua |
| author_sort | Yosef G. Kidane |
| collection | DOAJ |
| description | Septoria tritici blotch (STB) is a devastating fungal disease affecting durum and bread wheat cultivation worldwide. The identification, development, and employment of resistant wheat genetic material is the key to overcoming costs and limitations of fungicide treatments. The search for resistance sources in untapped genetic material may speed up the deployment of STB genetic resistance in the field. Ethiopian durum wheat landraces represent a valuable source of such diversity. In this study, 318 Ethiopian durum wheat genotypes, for the most part traditional landraces, were phenotyped for resistance to different aspects of STB infection. Phenology, yield and yield component traits were concurrently measured the collection. Here we describe the distribution of STB resistance traits in modern varieties and in landraces, and the relation existing between STB resistance and other agronomic traits. STB resistance sources were found in landraces as well as in modern varieties tested, suggesting the presence of alleles of breeding relevance. The genetic material was genotyped with more than 16 thousand genome-wide polymorphic markers to describe the linkage disequilibrium and genetic structure existing within the panel of genotypes, and a genome-wide association (GWA) study was run to allow the identification of genomic loci involved in STB resistance. High diversity and low genetic structure in the panel allowed high efficiency GWA. The GWA scan detected five major putative QTL for STB resistance, only partially overlapping those already reported in the wheat literature. We report four putative loci for Septoria resistance with no match in previous literature: two highly significant ones on Chr 3A and 5A, and two suggestive ones on Chr 4B and 5B. Markers underlying these QTL explained as much as 10% of the phenotypic variance for disease resistance. We found three cases in which putative QTL for agronomic traits overlapped marker trait association deriving from STB GWA. Our results show that the Ethiopian untapped allelic diversity bears a great value in studying the molecular basis of STB resistance and in breeding for resistance in local and international material. |
| first_indexed | 2024-12-23T05:25:01Z |
| format | Article |
| id | doaj.art-9b99f59d2dc04e2dafd486d620fb0977 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T05:25:01Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-9b99f59d2dc04e2dafd486d620fb09772022-12-21T17:58:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-09-01810.3389/fpls.2017.01586270083Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat LandracesYosef G. Kidane0Yosef G. Kidane1Yosef G. Kidane2Bogale N. Hailemariam3Dejene K. Mengistu4Dejene K. Mengistu5Carlo Fadda6Mario Enrico Pè7Matteo Dell'Acqua8Institute of Life Sciences, Scuola Superiore Sant'AnnaPisa, ItalySirinka Agricultural Research CenterWoldia, EthiopiaBioversity InternationalAddis Ababa, EthiopiaInstitute of Life Sciences, Scuola Superiore Sant'AnnaPisa, ItalyInstitute of Life Sciences, Scuola Superiore Sant'AnnaPisa, ItalyDepartment of Dryland Crop and Horticultural Sciences, Mekelle UniversityMekelle, EthiopiaBioversity InternationalAddis Ababa, EthiopiaInstitute of Life Sciences, Scuola Superiore Sant'AnnaPisa, ItalyInstitute of Life Sciences, Scuola Superiore Sant'AnnaPisa, ItalySeptoria tritici blotch (STB) is a devastating fungal disease affecting durum and bread wheat cultivation worldwide. The identification, development, and employment of resistant wheat genetic material is the key to overcoming costs and limitations of fungicide treatments. The search for resistance sources in untapped genetic material may speed up the deployment of STB genetic resistance in the field. Ethiopian durum wheat landraces represent a valuable source of such diversity. In this study, 318 Ethiopian durum wheat genotypes, for the most part traditional landraces, were phenotyped for resistance to different aspects of STB infection. Phenology, yield and yield component traits were concurrently measured the collection. Here we describe the distribution of STB resistance traits in modern varieties and in landraces, and the relation existing between STB resistance and other agronomic traits. STB resistance sources were found in landraces as well as in modern varieties tested, suggesting the presence of alleles of breeding relevance. The genetic material was genotyped with more than 16 thousand genome-wide polymorphic markers to describe the linkage disequilibrium and genetic structure existing within the panel of genotypes, and a genome-wide association (GWA) study was run to allow the identification of genomic loci involved in STB resistance. High diversity and low genetic structure in the panel allowed high efficiency GWA. The GWA scan detected five major putative QTL for STB resistance, only partially overlapping those already reported in the wheat literature. We report four putative loci for Septoria resistance with no match in previous literature: two highly significant ones on Chr 3A and 5A, and two suggestive ones on Chr 4B and 5B. Markers underlying these QTL explained as much as 10% of the phenotypic variance for disease resistance. We found three cases in which putative QTL for agronomic traits overlapped marker trait association deriving from STB GWA. Our results show that the Ethiopian untapped allelic diversity bears a great value in studying the molecular basis of STB resistance and in breeding for resistance in local and international material.http://journal.frontiersin.org/article/10.3389/fpls.2017.01586/fullGWASZymoseptoria triticiMycosphaerella graminicoladurum wheatQTL mappinglandraces |
| spellingShingle | Yosef G. Kidane Yosef G. Kidane Yosef G. Kidane Bogale N. Hailemariam Dejene K. Mengistu Dejene K. Mengistu Carlo Fadda Mario Enrico Pè Matteo Dell'Acqua Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces Frontiers in Plant Science GWAS Zymoseptoria tritici Mycosphaerella graminicola durum wheat QTL mapping landraces |
| title | Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces |
| title_full | Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces |
| title_fullStr | Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces |
| title_full_unstemmed | Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces |
| title_short | Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces |
| title_sort | genome wide association study of septoria tritici blotch resistance in ethiopian durum wheat landraces |
| topic | GWAS Zymoseptoria tritici Mycosphaerella graminicola durum wheat QTL mapping landraces |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01586/full |
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