Heterozygosity of the Complex Corfu δ<sup>0</sup>β<sup>+</sup> Thalassemic Allele (<i>HBD</i> Deletion and HBB:c.92+5G>A) Revisited

The Corfu δ<sup>0</sup>β<sup>+</sup> thalassemic allele is a unique thalassemic allele consisting of the simultaneous presence in cis of a deletion of the δ-globin (Hemoglobin Subunit Delta, <i>HBD</i>) and a single nucleotide variant in the β-globin gene (Hemoglo...

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Main Authors: Christos Kattamis, Myrto Skafida, Polyxeni Delaporta, Christina Vrettou, Joanne Traeger-Synodinos, Christalena Sofocleous, Antonis Kattamis
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Biology
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Online Access:https://www.mdpi.com/2079-7737/11/3/432
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author Christos Kattamis
Myrto Skafida
Polyxeni Delaporta
Christina Vrettou
Joanne Traeger-Synodinos
Christalena Sofocleous
Antonis Kattamis
author_facet Christos Kattamis
Myrto Skafida
Polyxeni Delaporta
Christina Vrettou
Joanne Traeger-Synodinos
Christalena Sofocleous
Antonis Kattamis
author_sort Christos Kattamis
collection DOAJ
description The Corfu δ<sup>0</sup>β<sup>+</sup> thalassemic allele is a unique thalassemic allele consisting of the simultaneous presence in cis of a deletion of the δ-globin (Hemoglobin Subunit Delta, <i>HBD</i>) and a single nucleotide variant in the β-globin gene (Hemoglobin Subunit Beta, <i>HBB</i>). The allele has, so far, been described in individuals of Greek origin. The objectives of the study are to ascertain the prevalence of the Corfu δ<sup>0</sup>β<sup>+</sup> allele in comparison to other β-thalassemia variants encountered in Greece using our in-house data repository of 2558 β-thalassemia heterozygotes, and to evaluate the hematological phenotype of Corfu δ<sup>0</sup>β<sup>+</sup> heterozygotes in comparison to heterozygotes with the most common β<sup>+</sup>- and deletion α<sup>0</sup>- thalassemia variants in Greece. The results of the study showed a relative incidence of heterozygotes with Corfu δ<sup>0</sup>β<sup>+</sup> at 1.56% of all β-thalassemic alleles, and a distinct hematological phenotype of the heterozygotes characterized by microcytic, hypochromic anemia with normal levels of HbA<sub>2</sub> (Hemoglobin A2) and elevated HbF (Hemoglobin F) levels. The application of a specific methodology for the identification of the Corfu δ<sup>0</sup>β<sup>+</sup> allele is important for precise prenatal and antenatal diagnosis programs in Greece.
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spelling doaj.art-9b9ba70b1ac34385b5dce4bd49dc0a372023-11-30T20:52:07ZengMDPI AGBiology2079-77372022-03-0111343210.3390/biology11030432Heterozygosity of the Complex Corfu δ<sup>0</sup>β<sup>+</sup> Thalassemic Allele (<i>HBD</i> Deletion and HBB:c.92+5G>A) RevisitedChristos Kattamis0Myrto Skafida1Polyxeni Delaporta2Christina Vrettou3Joanne Traeger-Synodinos4Christalena Sofocleous5Antonis Kattamis6Thalassemia Unit, Division Pediatric Hematology-Oncology, First Department of Pediatrics, National & Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, 11527 Athens, GreeceThalassemia Unit, Division Pediatric Hematology-Oncology, First Department of Pediatrics, National & Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, 11527 Athens, GreeceThalassemia Unit, Division Pediatric Hematology-Oncology, First Department of Pediatrics, National & Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, 11527 Athens, GreeceLaboratory of Medical Genetics, National & Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, 11527 Athens, GreeceLaboratory of Medical Genetics, National & Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, 11527 Athens, GreeceLaboratory of Medical Genetics, National & Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, 11527 Athens, GreeceThalassemia Unit, Division Pediatric Hematology-Oncology, First Department of Pediatrics, National & Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, 11527 Athens, GreeceThe Corfu δ<sup>0</sup>β<sup>+</sup> thalassemic allele is a unique thalassemic allele consisting of the simultaneous presence in cis of a deletion of the δ-globin (Hemoglobin Subunit Delta, <i>HBD</i>) and a single nucleotide variant in the β-globin gene (Hemoglobin Subunit Beta, <i>HBB</i>). The allele has, so far, been described in individuals of Greek origin. The objectives of the study are to ascertain the prevalence of the Corfu δ<sup>0</sup>β<sup>+</sup> allele in comparison to other β-thalassemia variants encountered in Greece using our in-house data repository of 2558 β-thalassemia heterozygotes, and to evaluate the hematological phenotype of Corfu δ<sup>0</sup>β<sup>+</sup> heterozygotes in comparison to heterozygotes with the most common β<sup>+</sup>- and deletion α<sup>0</sup>- thalassemia variants in Greece. The results of the study showed a relative incidence of heterozygotes with Corfu δ<sup>0</sup>β<sup>+</sup> at 1.56% of all β-thalassemic alleles, and a distinct hematological phenotype of the heterozygotes characterized by microcytic, hypochromic anemia with normal levels of HbA<sub>2</sub> (Hemoglobin A2) and elevated HbF (Hemoglobin F) levels. The application of a specific methodology for the identification of the Corfu δ<sup>0</sup>β<sup>+</sup> allele is important for precise prenatal and antenatal diagnosis programs in Greece.https://www.mdpi.com/2079-7737/11/3/432Corfu δ<sup>0</sup>β<sup>+</sup> thalassemic alleleβ-thalassemia variantsβ-thal hematological phenotypenormal HbA2high HbF
spellingShingle Christos Kattamis
Myrto Skafida
Polyxeni Delaporta
Christina Vrettou
Joanne Traeger-Synodinos
Christalena Sofocleous
Antonis Kattamis
Heterozygosity of the Complex Corfu δ<sup>0</sup>β<sup>+</sup> Thalassemic Allele (<i>HBD</i> Deletion and HBB:c.92+5G>A) Revisited
Biology
Corfu δ<sup>0</sup>β<sup>+</sup> thalassemic allele
β-thalassemia variants
β-thal hematological phenotype
normal HbA2
high HbF
title Heterozygosity of the Complex Corfu δ<sup>0</sup>β<sup>+</sup> Thalassemic Allele (<i>HBD</i> Deletion and HBB:c.92+5G>A) Revisited
title_full Heterozygosity of the Complex Corfu δ<sup>0</sup>β<sup>+</sup> Thalassemic Allele (<i>HBD</i> Deletion and HBB:c.92+5G>A) Revisited
title_fullStr Heterozygosity of the Complex Corfu δ<sup>0</sup>β<sup>+</sup> Thalassemic Allele (<i>HBD</i> Deletion and HBB:c.92+5G>A) Revisited
title_full_unstemmed Heterozygosity of the Complex Corfu δ<sup>0</sup>β<sup>+</sup> Thalassemic Allele (<i>HBD</i> Deletion and HBB:c.92+5G>A) Revisited
title_short Heterozygosity of the Complex Corfu δ<sup>0</sup>β<sup>+</sup> Thalassemic Allele (<i>HBD</i> Deletion and HBB:c.92+5G>A) Revisited
title_sort heterozygosity of the complex corfu δ sup 0 sup β sup sup thalassemic allele i hbd i deletion and hbb c 92 5g a revisited
topic Corfu δ<sup>0</sup>β<sup>+</sup> thalassemic allele
β-thalassemia variants
β-thal hematological phenotype
normal HbA2
high HbF
url https://www.mdpi.com/2079-7737/11/3/432
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