Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probiotics
The current study was undertaken to investigate the role of macrophages as a cellular immune function against immunization with whole sonicated <em>Listeria monocytogenes</em> antigens (WSLMAgs) and the effect of probiotics. A preparation of WSLMAgs containing whole <em>L. monocyto...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2021-12-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_170207_254fc8ab7ec7f69df21e3afa2ea2d9e1.pdf |
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| author | Thamer J. Shihab Zainab I. Ibrahim |
| author_facet | Thamer J. Shihab Zainab I. Ibrahim |
| author_sort | Thamer J. Shihab |
| collection | DOAJ |
| description | The current study was undertaken to investigate the role of macrophages as a cellular immune function against immunization with whole sonicated <em>Listeria monocytogenes</em> antigens (WSLMAgs) and the effect of probiotics. A preparation of WSLMAgs containing whole <em>L. monocytogenes </em>cell, after two subcutaneous immunization of BALB/c mice with 0.5ml WSLMAgs (0.5mg/ml) at an interval of two weeks. The bacterial identification was conducted by a conventional culture method using <em>Listeria</em> selective media PALCAM and confirmed by Polymerase Chain Reaction (PCR), As well as, the immunohistochemical and pathological change of it was studied in vivo by inoculating mice with pre-challenge WSLMAgs and post-challenge with virulent <em>L. monocytogenes</em> (1×10<sup>8</sup> CFU/mL). The results revealed the cellular immune function against pre- and post-immunization in spleen organ via lymphocytic hyperplasia in white pulp and coalescence of lymphoid follicles and marker F4/80+ show the immune-positive cells in aggregation adjacent to lymphoid follicle or focal aggregation of macrophages between follicles. In conclusion, the effectiveness of sonicated <em>L. monocytogenes </em>pre and post-immunization then challenge with virulent <em>L. monocytogenes</em> in the induction of cellular immune response, might serve as an immunization platform for applicants. |
| first_indexed | 2024-04-12T04:39:12Z |
| format | Article |
| id | doaj.art-9b9bf7d774d64079b4d526e9dc2dd2c2 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-12T04:39:12Z |
| publishDate | 2021-12-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-9b9bf7d774d64079b4d526e9dc2dd2c22022-12-22T03:47:42ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552021-12-0135Supplement I-III798510.33899/ijvs.2021.131562.1970170207Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probioticsThamer J. Shihab0Zainab I. Ibrahim1Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Baghdad, IraqDepartment of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, IraqThe current study was undertaken to investigate the role of macrophages as a cellular immune function against immunization with whole sonicated <em>Listeria monocytogenes</em> antigens (WSLMAgs) and the effect of probiotics. A preparation of WSLMAgs containing whole <em>L. monocytogenes </em>cell, after two subcutaneous immunization of BALB/c mice with 0.5ml WSLMAgs (0.5mg/ml) at an interval of two weeks. The bacterial identification was conducted by a conventional culture method using <em>Listeria</em> selective media PALCAM and confirmed by Polymerase Chain Reaction (PCR), As well as, the immunohistochemical and pathological change of it was studied in vivo by inoculating mice with pre-challenge WSLMAgs and post-challenge with virulent <em>L. monocytogenes</em> (1×10<sup>8</sup> CFU/mL). The results revealed the cellular immune function against pre- and post-immunization in spleen organ via lymphocytic hyperplasia in white pulp and coalescence of lymphoid follicles and marker F4/80+ show the immune-positive cells in aggregation adjacent to lymphoid follicle or focal aggregation of macrophages between follicles. In conclusion, the effectiveness of sonicated <em>L. monocytogenes </em>pre and post-immunization then challenge with virulent <em>L. monocytogenes</em> in the induction of cellular immune response, might serve as an immunization platform for applicants.https://www.vetmedmosul.com/article_170207_254fc8ab7ec7f69df21e3afa2ea2d9e1.pdflisteria monocytogenesantigensprobioticsmacrophageimmuno-histochemical (ihc) |
| spellingShingle | Thamer J. Shihab Zainab I. Ibrahim Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probiotics Iraqi Journal of Veterinary Sciences listeria monocytogenes antigens probiotics macrophage immuno-histochemical (ihc) |
| title | Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probiotics |
| title_full | Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probiotics |
| title_fullStr | Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probiotics |
| title_full_unstemmed | Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probiotics |
| title_short | Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probiotics |
| title_sort | immunohistochemical and pathological changes in balb c mice immunized with whole sonicated listeria monocytogenes antigens and the effect of probiotics |
| topic | listeria monocytogenes antigens probiotics macrophage immuno-histochemical (ihc) |
| url | https://www.vetmedmosul.com/article_170207_254fc8ab7ec7f69df21e3afa2ea2d9e1.pdf |
| work_keys_str_mv | AT thamerjshihab immunohistochemicalandpathologicalchangesinbalbcmiceimmunizedwithwholesonicatedlisteriamonocytogenesantigensandtheeffectofprobiotics AT zainabiibrahim immunohistochemicalandpathologicalchangesinbalbcmiceimmunizedwithwholesonicatedlisteriamonocytogenesantigensandtheeffectofprobiotics |