Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water
The west coast of the Arabian Peninsula borders the Red Sea, a water body which maintains high average temperatures and increased salinity compared to other seas or oceans. This geography has many resources which could be used to support algal biotechnology efforts in bio-resource circularity. Howev...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1157151/full |
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| author | Melany Villegas-Valencia Ricardo E. González-Portela Bárbara Bastos de Freitas Abdulaziz Al Jahdali Gabriel I. Romero-Villegas Raghdah Malibari Rahul Vijay Kapoore Claudio Fuentes-Grünewald Kyle J. Lauersen |
| author_facet | Melany Villegas-Valencia Ricardo E. González-Portela Bárbara Bastos de Freitas Abdulaziz Al Jahdali Gabriel I. Romero-Villegas Raghdah Malibari Rahul Vijay Kapoore Claudio Fuentes-Grünewald Kyle J. Lauersen |
| author_sort | Melany Villegas-Valencia |
| collection | DOAJ |
| description | The west coast of the Arabian Peninsula borders the Red Sea, a water body which maintains high average temperatures and increased salinity compared to other seas or oceans. This geography has many resources which could be used to support algal biotechnology efforts in bio-resource circularity. However, summer conditions in this region may exceed the temperature tolerance of most currently cultivated microalgae. The Cyanidiophyceae are a class of polyextremophilic red algae that natively inhabit acidic hot springs. C. merolae 10D has recently emerged as an interesting model organism capable of high-cell density cultivation on pure CO2 with optimal growth at elevated temperatures and acidic pH. C. merolae biomass has an interesting macromolecular composition, is protein rich, and contains valuable bio-products like heat-stable phycocyanin, carotenoids, β-glucan, and starch. Here, photobioreactors were used to model C. merolae 10D growth performance in simulated environmental conditions of the mid-Red Sea coast across four seasons, it was then grown at various scales outdoors in Thuwal, Saudi Arabia during the Summer of 2022. We show that C. merolae 10D is amenable to cultivation with industrial-grade nutrient and CO2 inputs outdoors in this location and that its biomass is relatively constant in biochemical composition across culture conditions. We also show the adaptation of C. merolae 10D to high salinity levels of those found in Red Sea waters and conducted further modeled cultivations in nutrient enriched local sea water. It was determined that salt-water adapted C. merolae 10D could be cultivated with reduced nutrient inputs in local conditions. The results presented here indicate this may be a promising alternative species for algal bioprocesses in outdoor conditions in extreme coastal desert summer environments. |
| first_indexed | 2024-04-09T17:05:34Z |
| format | Article |
| id | doaj.art-9b9c12d55bc14c1d97729927975d5f9b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T17:05:34Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9b9c12d55bc14c1d97729927975d5f9b2023-04-20T16:01:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11571511157151Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea waterMelany Villegas-Valencia0Ricardo E. González-Portela1Bárbara Bastos de Freitas2Abdulaziz Al Jahdali3Gabriel I. Romero-Villegas4Raghdah Malibari5Rahul Vijay Kapoore6Claudio Fuentes-Grünewald7Kyle J. Lauersen8Bioengineering Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDevelopment of Algal Biotechnology in Kingdom of Saudi Arabia (DAB-KSA) Project, Beacon Development, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaBioengineering Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDevelopment of Algal Biotechnology in Kingdom of Saudi Arabia (DAB-KSA) Project, Beacon Development, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDevelopment of Algal Biotechnology in Kingdom of Saudi Arabia (DAB-KSA) Project, Beacon Development, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDevelopment of Algal Biotechnology in Kingdom of Saudi Arabia (DAB-KSA) Project, Beacon Development, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDevelopment of Algal Biotechnology in Kingdom of Saudi Arabia (DAB-KSA) Project, Beacon Development, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDevelopment of Algal Biotechnology in Kingdom of Saudi Arabia (DAB-KSA) Project, Beacon Development, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaBioengineering Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaThe west coast of the Arabian Peninsula borders the Red Sea, a water body which maintains high average temperatures and increased salinity compared to other seas or oceans. This geography has many resources which could be used to support algal biotechnology efforts in bio-resource circularity. However, summer conditions in this region may exceed the temperature tolerance of most currently cultivated microalgae. The Cyanidiophyceae are a class of polyextremophilic red algae that natively inhabit acidic hot springs. C. merolae 10D has recently emerged as an interesting model organism capable of high-cell density cultivation on pure CO2 with optimal growth at elevated temperatures and acidic pH. C. merolae biomass has an interesting macromolecular composition, is protein rich, and contains valuable bio-products like heat-stable phycocyanin, carotenoids, β-glucan, and starch. Here, photobioreactors were used to model C. merolae 10D growth performance in simulated environmental conditions of the mid-Red Sea coast across four seasons, it was then grown at various scales outdoors in Thuwal, Saudi Arabia during the Summer of 2022. We show that C. merolae 10D is amenable to cultivation with industrial-grade nutrient and CO2 inputs outdoors in this location and that its biomass is relatively constant in biochemical composition across culture conditions. We also show the adaptation of C. merolae 10D to high salinity levels of those found in Red Sea waters and conducted further modeled cultivations in nutrient enriched local sea water. It was determined that salt-water adapted C. merolae 10D could be cultivated with reduced nutrient inputs in local conditions. The results presented here indicate this may be a promising alternative species for algal bioprocesses in outdoor conditions in extreme coastal desert summer environments.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1157151/fullcircular resource biotechnologydesertCyanidioschyzon merolae 10Dsea waterSaudi Arabia Red Sea coastalgal biotechnology |
| spellingShingle | Melany Villegas-Valencia Ricardo E. González-Portela Bárbara Bastos de Freitas Abdulaziz Al Jahdali Gabriel I. Romero-Villegas Raghdah Malibari Rahul Vijay Kapoore Claudio Fuentes-Grünewald Kyle J. Lauersen Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water Frontiers in Microbiology circular resource biotechnology desert Cyanidioschyzon merolae 10D sea water Saudi Arabia Red Sea coast algal biotechnology |
| title | Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water |
| title_full | Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water |
| title_fullStr | Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water |
| title_full_unstemmed | Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water |
| title_short | Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water |
| title_sort | cultivation of the polyextremophile cyanidioschyzon merolae 10d during summer conditions on the coast of the red sea and its adaptation to hypersaline sea water |
| topic | circular resource biotechnology desert Cyanidioschyzon merolae 10D sea water Saudi Arabia Red Sea coast algal biotechnology |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1157151/full |
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