Evaluation of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and Malignancy
<i>Usnea</i> lichens are known for their beneficial pharmacological effects with potential applications in oral medicine. This study aims to investigate the extract of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from the Călimani Mountains in canola oil as an oral pharmaceut...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/8/1601 |
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| author | Violeta Popovici Elena Matei Georgeta Camelia Cozaru Laura Bucur Cerasela Elena Gîrd Verginica Schröder Emma Adriana Ozon Oana Karampelas Adina Magdalena Musuc Irina Atkinson Adriana Rusu Simona Petrescu Raul-Augustin Mitran Mihai Anastasescu Aureliana Caraiane Dumitru Lupuliasa Mariana Aschie Victoria Badea |
| author_facet | Violeta Popovici Elena Matei Georgeta Camelia Cozaru Laura Bucur Cerasela Elena Gîrd Verginica Schröder Emma Adriana Ozon Oana Karampelas Adina Magdalena Musuc Irina Atkinson Adriana Rusu Simona Petrescu Raul-Augustin Mitran Mihai Anastasescu Aureliana Caraiane Dumitru Lupuliasa Mariana Aschie Victoria Badea |
| author_sort | Violeta Popovici |
| collection | DOAJ |
| description | <i>Usnea</i> lichens are known for their beneficial pharmacological effects with potential applications in oral medicine. This study aims to investigate the extract of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from the Călimani Mountains in canola oil as an oral pharmaceutical formulation. In the present work, bioadhesive oral films (F-UBO) with <i>U. barbata</i> extract in canola oil (UBO) were formulated, characterized, and evaluated, evidencing their pharmacological potential. The UBO-loaded films were analyzed using standard methods regarding physicochemical and pharmacotechnical characteristics to verify their suitability for topical administration on the oral mucosa. F-UBO suitability confirmation allowed for the investigation of antimicrobial and anticancer potential. The antimicrobial properties against <i>Staphylococcus aureus</i> ATCC 25923, <i>Pseudomonas aeruginosa</i> ATCC 27353, <i>Candida albicans</i> ATCC 10231, and <i>Candida parapsilosis</i> ATCC 22019 were evaluated by a resazurin-based 96-well plate microdilution method. The brine shrimp lethality assay (BSL assay) was the animal model cytotoxicity prescreen, followed by flow cytometry analyses on normal blood cells and oral epithelial squamous cell carcinoma CLS-354 cell line, determining cellular apoptosis, caspase-3/7 activity, nuclear condensation and lysosomal activity, oxidative stress, cell cycle, and cell proliferation. The results indicate that a UBO-loaded bioadhesive film’s weight is 63 ± 1.79 mg. It contains 315 µg UBO, has a pH = 6.97 ± 0.01, a disintegration time of 124 ± 3.67 s, and a bioadhesion time of 86 ± 4.12 min, being suitable for topical administration on the oral mucosa. F-UBO showed moderate dose-dependent inhibitory effects on the growth of both bacterial and fungal strains. Moreover, in CLS-354 tumor cells, F-UBO increased oxidative stress, diminished DNA synthesis, and induced cell cycle arrest in G0/G1. All these properties led to considering UBO-loaded bioadhesive oral films as a suitable phytotherapeutic formulation with potential application in oral infections and neoplasia. |
| first_indexed | 2024-03-09T11:58:45Z |
| format | Article |
| id | doaj.art-9ba30d2ec9914dfa99c501c69234d6ee |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:58:45Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-9ba30d2ec9914dfa99c501c69234d6ee2023-11-30T23:06:41ZengMDPI AGAntioxidants2076-39212022-08-01118160110.3390/antiox11081601Evaluation of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and MalignancyVioleta Popovici0Elena Matei1Georgeta Camelia Cozaru2Laura Bucur3Cerasela Elena Gîrd4Verginica Schröder5Emma Adriana Ozon6Oana Karampelas7Adina Magdalena Musuc8Irina Atkinson9Adriana Rusu10Simona Petrescu11Raul-Augustin Mitran12Mihai Anastasescu13Aureliana Caraiane14Dumitru Lupuliasa15Mariana Aschie16Victoria Badea17Department of Microbiology and Immunology, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, RomaniaCenter for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, Ovidius University of Constanta, CEDMOG, 145 Tomis Blvd., 900591 Constanta, RomaniaCenter for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, Ovidius University of Constanta, CEDMOG, 145 Tomis Blvd., 900591 Constanta, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, Ovidius University of Constanta, 6 Capitan Al. Serbanescu Street, 900001 Constanta, RomaniaDepartment of Pharmacognosy, Phytochemistry, and Phytotherapy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956 Bucharest, RomaniaDepartment of Cellular and Molecular Biology, Faculty of Pharmacy, Ovidius University of Constanta, 6 Capitan Al. Serbanescu Street, 900001 Constanta, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956 Bucharest, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry, Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry, Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry, Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry, Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry, Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry, Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaDepartment of Oral Rehabilitation, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956 Bucharest, RomaniaCenter for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, Ovidius University of Constanta, CEDMOG, 145 Tomis Blvd., 900591 Constanta, RomaniaDepartment of Microbiology and Immunology, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, Romania<i>Usnea</i> lichens are known for their beneficial pharmacological effects with potential applications in oral medicine. This study aims to investigate the extract of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg from the Călimani Mountains in canola oil as an oral pharmaceutical formulation. In the present work, bioadhesive oral films (F-UBO) with <i>U. barbata</i> extract in canola oil (UBO) were formulated, characterized, and evaluated, evidencing their pharmacological potential. The UBO-loaded films were analyzed using standard methods regarding physicochemical and pharmacotechnical characteristics to verify their suitability for topical administration on the oral mucosa. F-UBO suitability confirmation allowed for the investigation of antimicrobial and anticancer potential. The antimicrobial properties against <i>Staphylococcus aureus</i> ATCC 25923, <i>Pseudomonas aeruginosa</i> ATCC 27353, <i>Candida albicans</i> ATCC 10231, and <i>Candida parapsilosis</i> ATCC 22019 were evaluated by a resazurin-based 96-well plate microdilution method. The brine shrimp lethality assay (BSL assay) was the animal model cytotoxicity prescreen, followed by flow cytometry analyses on normal blood cells and oral epithelial squamous cell carcinoma CLS-354 cell line, determining cellular apoptosis, caspase-3/7 activity, nuclear condensation and lysosomal activity, oxidative stress, cell cycle, and cell proliferation. The results indicate that a UBO-loaded bioadhesive film’s weight is 63 ± 1.79 mg. It contains 315 µg UBO, has a pH = 6.97 ± 0.01, a disintegration time of 124 ± 3.67 s, and a bioadhesion time of 86 ± 4.12 min, being suitable for topical administration on the oral mucosa. F-UBO showed moderate dose-dependent inhibitory effects on the growth of both bacterial and fungal strains. Moreover, in CLS-354 tumor cells, F-UBO increased oxidative stress, diminished DNA synthesis, and induced cell cycle arrest in G0/G1. All these properties led to considering UBO-loaded bioadhesive oral films as a suitable phytotherapeutic formulation with potential application in oral infections and neoplasia.https://www.mdpi.com/2076-3921/11/8/1601<i>Usnea barbata</i> (L.) Weber ex F.H. Wiggcanola oilbioadhesive oral filmspoloxamer 407antimicrobial activity<i>Artemia salina</i> |
| spellingShingle | Violeta Popovici Elena Matei Georgeta Camelia Cozaru Laura Bucur Cerasela Elena Gîrd Verginica Schröder Emma Adriana Ozon Oana Karampelas Adina Magdalena Musuc Irina Atkinson Adriana Rusu Simona Petrescu Raul-Augustin Mitran Mihai Anastasescu Aureliana Caraiane Dumitru Lupuliasa Mariana Aschie Victoria Badea Evaluation of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and Malignancy Antioxidants <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg canola oil bioadhesive oral films poloxamer 407 antimicrobial activity <i>Artemia salina</i> |
| title | Evaluation of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and Malignancy |
| title_full | Evaluation of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and Malignancy |
| title_fullStr | Evaluation of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and Malignancy |
| title_full_unstemmed | Evaluation of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and Malignancy |
| title_short | Evaluation of <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and Malignancy |
| title_sort | evaluation of i usnea barbata i l weber ex f h wigg extract in canola oil loaded in bioadhesive oral films for potential applications in oral cavity infections and malignancy |
| topic | <i>Usnea barbata</i> (L.) Weber ex F.H. Wigg canola oil bioadhesive oral films poloxamer 407 antimicrobial activity <i>Artemia salina</i> |
| url | https://www.mdpi.com/2076-3921/11/8/1601 |
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