In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed <i>Caulerpa cupressoides</i> var <i>flabellata</i>
Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals. Currently, there is no efficient compound that can be used to prevent this disease. However, seaweeds’ sulfated polysaccharides (SPs) can change the CaOx crystals...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/6/326 |
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| author | Dayanne Lopes Gomes Karoline Rachel Teodosio Melo Moacir Fernandes Queiroz Lucas Alighieri Neves Costa Batista Pablo Castro Santos Mariana Santana Santos Pereira Costa Jailma Almeida-Lima Rafael Barros Gomes Camara Leandro Silva Costa Hugo Alexandre Oliveira Rocha |
| author_facet | Dayanne Lopes Gomes Karoline Rachel Teodosio Melo Moacir Fernandes Queiroz Lucas Alighieri Neves Costa Batista Pablo Castro Santos Mariana Santana Santos Pereira Costa Jailma Almeida-Lima Rafael Barros Gomes Camara Leandro Silva Costa Hugo Alexandre Oliveira Rocha |
| author_sort | Dayanne Lopes Gomes |
| collection | DOAJ |
| description | Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals. Currently, there is no efficient compound that can be used to prevent this disease. However, seaweeds’ sulfated polysaccharides (SPs) can change the CaOx crystals surface’s charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis. We observed that the SPs of <i>Caulerpa cupressoides</i> modified the morphology, size and surface charge of CaOx crystals. Thus, these crystals became similar to those found in healthy persons. In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology. Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa’s SPs and the CaOx crystals. This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones. |
| first_indexed | 2024-04-14T04:45:44Z |
| format | Article |
| id | doaj.art-9ba481caf9ad4ac6a5bcdb96b075e066 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T04:45:44Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-9ba481caf9ad4ac6a5bcdb96b075e0662022-12-22T02:11:27ZengMDPI AGMarine Drugs1660-33972019-06-0117632610.3390/md17060326md17060326In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed <i>Caulerpa cupressoides</i> var <i>flabellata</i>Dayanne Lopes Gomes0Karoline Rachel Teodosio Melo1Moacir Fernandes Queiroz2Lucas Alighieri Neves Costa Batista3Pablo Castro Santos4Mariana Santana Santos Pereira Costa5Jailma Almeida-Lima6Rafael Barros Gomes Camara7Leandro Silva Costa8Hugo Alexandre Oliveira Rocha9Laboratory of Natural Polymer Biotechnology (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, BrazilLaboratory of Natural Polymer Biotechnology (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, BrazilLaboratory of Natural Polymer Biotechnology (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, BrazilLaboratory of Natural Polymer Biotechnology (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, BrazilState University of Rio Grande do Norte (UERN), Mossoró-RN 59.610-210, BrazilFederal Institute of Education, Science and Technology of Rio Grande do Norte (IFRN), João Câmara-RN 59.550-000, BrazilLaboratory of Natural Polymer Biotechnology (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, BrazilLaboratory of Natural Polymer Biotechnology (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, BrazilFederal Institute of Education, Science and Technology of Rio Grande do Norte (IFRN), Canguaretama-RN 59.190-000, BrazilLaboratory of Natural Polymer Biotechnology (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, BrazilUrolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals. Currently, there is no efficient compound that can be used to prevent this disease. However, seaweeds’ sulfated polysaccharides (SPs) can change the CaOx crystals surface’s charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis. We observed that the SPs of <i>Caulerpa cupressoides</i> modified the morphology, size and surface charge of CaOx crystals. Thus, these crystals became similar to those found in healthy persons. In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology. Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa’s SPs and the CaOx crystals. This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.https://www.mdpi.com/1660-3397/17/6/326urolithiasisgreen algaeCOD dumbbellCOD stabilization |
| spellingShingle | Dayanne Lopes Gomes Karoline Rachel Teodosio Melo Moacir Fernandes Queiroz Lucas Alighieri Neves Costa Batista Pablo Castro Santos Mariana Santana Santos Pereira Costa Jailma Almeida-Lima Rafael Barros Gomes Camara Leandro Silva Costa Hugo Alexandre Oliveira Rocha In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed <i>Caulerpa cupressoides</i> var <i>flabellata</i> Marine Drugs urolithiasis green algae COD dumbbell COD stabilization |
| title | In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed <i>Caulerpa cupressoides</i> var <i>flabellata</i> |
| title_full | In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed <i>Caulerpa cupressoides</i> var <i>flabellata</i> |
| title_fullStr | In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed <i>Caulerpa cupressoides</i> var <i>flabellata</i> |
| title_full_unstemmed | In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed <i>Caulerpa cupressoides</i> var <i>flabellata</i> |
| title_short | In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed <i>Caulerpa cupressoides</i> var <i>flabellata</i> |
| title_sort | in vitro studies reveal antiurolithic effect of antioxidant sulfated polysaccharides from the green seaweed i caulerpa cupressoides i var i flabellata i |
| topic | urolithiasis green algae COD dumbbell COD stabilization |
| url | https://www.mdpi.com/1660-3397/17/6/326 |
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