In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit
Objective: To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr.fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo). Methods: Fruits of Citrus macroptera without rind was extracted wit...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-06-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115302252 |
| _version_ | 1818148970530078720 |
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| author | Nizam Uddin Md. Rakib Hasan Md. Monir Hossain Arjyabrata Sarker A.H.M. Nazmul Hasan A.F.M. Mahmudul Islam Mohd. Motaher H. Chowdhury Md. Sohel Rana |
| author_facet | Nizam Uddin Md. Rakib Hasan Md. Monir Hossain Arjyabrata Sarker A.H.M. Nazmul Hasan A.F.M. Mahmudul Islam Mohd. Motaher H. Chowdhury Md. Sohel Rana |
| author_sort | Nizam Uddin |
| collection | DOAJ |
| description | Objective: To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr.fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo).
Methods: Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.
Results: Presence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate α-amylase inhibitory activity [IC50 value=(3.638±0.190) mg/mL] as compared to acarbose. Moreover at 500 mg/kg and 1000 mg/kg doses fruit extract significantly (P<0.05 and P<0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg/kg). In oral glucose tolerance test, 500 mg/kg dose significantly reduced blood glucose level (P<0.05) at 2 h but 1000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h (P<0.05 and P<0.01 respectively) whereas glibenclamide (5 mg/kg) significantly reduced glucose level at every hour after administration. Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test.
Conclusion: These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent. |
| first_indexed | 2024-12-11T12:59:36Z |
| format | Article |
| id | doaj.art-9ba56d2aed1144c3ad08e3fef1488cb0 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-11T12:59:36Z |
| publishDate | 2014-06-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-9ba56d2aed1144c3ad08e3fef1488cb02022-12-22T01:06:30ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-06-014647347910.12980/APJTB.4.2014C1173In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruitNizam UddinMd. Rakib HasanMd. Monir HossainArjyabrata SarkerA.H.M. Nazmul HasanA.F.M. Mahmudul IslamMohd. Motaher H. ChowdhuryMd. Sohel RanaObjective: To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr.fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo). Methods: Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats. Results: Presence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate α-amylase inhibitory activity [IC50 value=(3.638±0.190) mg/mL] as compared to acarbose. Moreover at 500 mg/kg and 1000 mg/kg doses fruit extract significantly (P<0.05 and P<0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg/kg). In oral glucose tolerance test, 500 mg/kg dose significantly reduced blood glucose level (P<0.05) at 2 h but 1000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h (P<0.05 and P<0.01 respectively) whereas glibenclamide (5 mg/kg) significantly reduced glucose level at every hour after administration. Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test. Conclusion: These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.http://www.sciencedirect.com/science/article/pii/S2221169115302252Diabetes mellitusHypoglycemicCitrus macropteraα-AmylaseOGTTGlibenclamide |
| spellingShingle | Nizam Uddin Md. Rakib Hasan Md. Monir Hossain Arjyabrata Sarker A.H.M. Nazmul Hasan A.F.M. Mahmudul Islam Mohd. Motaher H. Chowdhury Md. Sohel Rana In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit Asian Pacific Journal of Tropical Biomedicine Diabetes mellitus Hypoglycemic Citrus macroptera α-Amylase OGTT Glibenclamide |
| title | In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit |
| title_full | In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit |
| title_fullStr | In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit |
| title_full_unstemmed | In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit |
| title_short | In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit |
| title_sort | in vitro α amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of citrus macroptera montr fruit |
| topic | Diabetes mellitus Hypoglycemic Citrus macroptera α-Amylase OGTT Glibenclamide |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115302252 |
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