Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells
Actin networks and actin-binding proteins (ABPs) are most abundant in the cytoskeleton of neurons. The function of ABPs in neurons is nucleation of actin polymerization, polymerization or depolymerization regulation, bundling of actin through crosslinking or stabilization, cargo movement along actin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2020.588556/full |
| _version_ | 1818384148318912512 |
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| author | Minkyo Jung Doory Kim Ji Young Mun |
| author_facet | Minkyo Jung Doory Kim Ji Young Mun |
| author_sort | Minkyo Jung |
| collection | DOAJ |
| description | Actin networks and actin-binding proteins (ABPs) are most abundant in the cytoskeleton of neurons. The function of ABPs in neurons is nucleation of actin polymerization, polymerization or depolymerization regulation, bundling of actin through crosslinking or stabilization, cargo movement along actin filaments, and anchoring of actin to other cellular components. In axons, ABP–actin interaction forms a dynamic, deep actin network, which regulates axon extension, guidance, axon branches, and synaptic structures. In dendrites, actin and ABPs are related to filopodia attenuation, spine formation, and synapse plasticity. ABP phosphorylation or mutation changes ABP–actin binding, which regulates axon or dendritic plasticity. In addition, hyperactive ABPs might also be expressed as aggregates of abnormal proteins in neurodegeneration. Those changes cause many neurological disorders. Here, we will review direct visualization of ABP and actin using various electron microscopy (EM) techniques, super resolution microscopy (SRM), and correlative light and electron microscopy (CLEM) with discussion of important ABPs in neuron. |
| first_indexed | 2024-12-14T03:17:39Z |
| format | Article |
| id | doaj.art-9ba8b8d3a2ec4b8aaa372ff44b61f762 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-14T03:17:39Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-9ba8b8d3a2ec4b8aaa372ff44b61f7622022-12-21T23:19:06ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-11-01810.3389/fcell.2020.588556588556Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal CellsMinkyo Jung0Doory Kim1Ji Young Mun2Neural Circuit Research Group, Korea Brain Research Institute, Daegu, South KoreaDepartment of Chemistry, Research Institute for Convergence of Basic Sciences, Institute of Nano Science and Technology, Research Institute for Natural Sciences, Hanyang University, Seoul, South KoreaNeural Circuit Research Group, Korea Brain Research Institute, Daegu, South KoreaActin networks and actin-binding proteins (ABPs) are most abundant in the cytoskeleton of neurons. The function of ABPs in neurons is nucleation of actin polymerization, polymerization or depolymerization regulation, bundling of actin through crosslinking or stabilization, cargo movement along actin filaments, and anchoring of actin to other cellular components. In axons, ABP–actin interaction forms a dynamic, deep actin network, which regulates axon extension, guidance, axon branches, and synaptic structures. In dendrites, actin and ABPs are related to filopodia attenuation, spine formation, and synapse plasticity. ABP phosphorylation or mutation changes ABP–actin binding, which regulates axon or dendritic plasticity. In addition, hyperactive ABPs might also be expressed as aggregates of abnormal proteins in neurodegeneration. Those changes cause many neurological disorders. Here, we will review direct visualization of ABP and actin using various electron microscopy (EM) techniques, super resolution microscopy (SRM), and correlative light and electron microscopy (CLEM) with discussion of important ABPs in neuron.https://www.frontiersin.org/articles/10.3389/fcell.2020.588556/fullelectron microscopysuper-resolution microscopycorrelative light and electron microscopyactin binding proteinactinneuronal cell |
| spellingShingle | Minkyo Jung Doory Kim Ji Young Mun Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells Frontiers in Cell and Developmental Biology electron microscopy super-resolution microscopy correlative light and electron microscopy actin binding protein actin neuronal cell |
| title | Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells |
| title_full | Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells |
| title_fullStr | Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells |
| title_full_unstemmed | Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells |
| title_short | Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells |
| title_sort | direct visualization of actin filaments and actin binding proteins in neuronal cells |
| topic | electron microscopy super-resolution microscopy correlative light and electron microscopy actin binding protein actin neuronal cell |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2020.588556/full |
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