Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy
Sinisan has been widely used to treat depression. However, its pharmacologically-effective constituents are largely unknown, and the pharmacological effects and clinical efficacies of Sinisan-containing processed medicinal herbs may change. To address these important issues, we developed an ultra-hi...
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MDPI AG
2018-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/23/12/3128 |
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| author | Jia Zhou Hao Cai Sicong Tu Yu Duan Ke Pei Yangyang Xu Jing Liu Minjie Niu Yating Zhang Lin Shen Qigang Zhou |
| author_facet | Jia Zhou Hao Cai Sicong Tu Yu Duan Ke Pei Yangyang Xu Jing Liu Minjie Niu Yating Zhang Lin Shen Qigang Zhou |
| author_sort | Jia Zhou |
| collection | DOAJ |
| description | Sinisan has been widely used to treat depression. However, its pharmacologically-effective constituents are largely unknown, and the pharmacological effects and clinical efficacies of Sinisan-containing processed medicinal herbs may change. To address these important issues, we developed an ultra-high performance liquid chromatography coupled with electrospray ionization tandem quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) method coupled with multiple statistical strategies to analyze the compound profiles of Sinisan, including individual herb, herb-pair, and complicated Chinese medicinal formula. As a result, 122 different constituents from individual herb, herb-pair, and complicated Chinese medicinal formula were identified totally. Through the comparison of three progressive levels, it suggests that processing herbal medicine and/or altering medicinal formula compatibility could change herbal chemical constituents, resulting in different pharmacological effects. This is also the first report that saikosaponin h/i and saikosaponin g have been identified in Sinisan. |
| first_indexed | 2024-12-14T10:05:32Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-14T10:05:32Z |
| publishDate | 2018-11-01 |
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| spelling | doaj.art-9baae309c354422e8fc2bafc35ddf7e02022-12-21T23:07:09ZengMDPI AGMolecules1420-30492018-11-012312312810.3390/molecules23123128molecules23123128Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical StrategyJia Zhou0Hao Cai1Sicong Tu2Yu Duan3Ke Pei4Yangyang Xu5Jing Liu6Minjie Niu7Yating Zhang8Lin Shen9Qigang Zhou10School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaMedical Sciences Division, University of Oxford, Oxford OX3 7BN, UKSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaInstitute of Pharmaceutical and Food Engineering, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing Medical University, Nanjing 210026, ChinaSinisan has been widely used to treat depression. However, its pharmacologically-effective constituents are largely unknown, and the pharmacological effects and clinical efficacies of Sinisan-containing processed medicinal herbs may change. To address these important issues, we developed an ultra-high performance liquid chromatography coupled with electrospray ionization tandem quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) method coupled with multiple statistical strategies to analyze the compound profiles of Sinisan, including individual herb, herb-pair, and complicated Chinese medicinal formula. As a result, 122 different constituents from individual herb, herb-pair, and complicated Chinese medicinal formula were identified totally. Through the comparison of three progressive levels, it suggests that processing herbal medicine and/or altering medicinal formula compatibility could change herbal chemical constituents, resulting in different pharmacological effects. This is also the first report that saikosaponin h/i and saikosaponin g have been identified in Sinisan.https://www.mdpi.com/1420-3049/23/12/3128chemical constituent profiles of Sinisanchinese medicine processingchinese medicinal formula compatibility |
| spellingShingle | Jia Zhou Hao Cai Sicong Tu Yu Duan Ke Pei Yangyang Xu Jing Liu Minjie Niu Yating Zhang Lin Shen Qigang Zhou Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy Molecules chemical constituent profiles of Sinisan chinese medicine processing chinese medicinal formula compatibility |
| title | Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy |
| title_full | Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy |
| title_fullStr | Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy |
| title_full_unstemmed | Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy |
| title_short | Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy |
| title_sort | identification and analysis of compound profiles of sinisan based on individual herb herb pair herbal formula before and after processing using uhplc q tof ms coupled with multiple statistical strategy |
| topic | chemical constituent profiles of Sinisan chinese medicine processing chinese medicinal formula compatibility |
| url | https://www.mdpi.com/1420-3049/23/12/3128 |
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