Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract
Gut microbiota provides protection against arsenic (As) induced toxicity, and As metabolism is considered an important part of risk assessment associated with soil As exposures. However, little is known about microbial iron(III) reduction and its role in metabolism of soil-bound As in the human gut....
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412023001848 |
| _version_ | 1797840025415581696 |
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| author | Naiyi Yin Xuhui Chang Peng Xiao Yi Zhou Xiaotong Liu Shimao Xiong Pengfei Wang Xiaolin Cai Guoxin Sun Yanshan Cui Zhengyi Hu |
| author_facet | Naiyi Yin Xuhui Chang Peng Xiao Yi Zhou Xiaotong Liu Shimao Xiong Pengfei Wang Xiaolin Cai Guoxin Sun Yanshan Cui Zhengyi Hu |
| author_sort | Naiyi Yin |
| collection | DOAJ |
| description | Gut microbiota provides protection against arsenic (As) induced toxicity, and As metabolism is considered an important part of risk assessment associated with soil As exposures. However, little is known about microbial iron(III) reduction and its role in metabolism of soil-bound As in the human gut. Here, we determined the dissolution and transformation of As and Fe from incidental ingestion of contaminated soils as a function of particle size (<250 μm, 100–250 μm, 50–100 μm and < 50 μm). Colon incubation with human gut microbiota yielded a high degree of As reduction and methylation of up to 53.4 and 0.074 μg/(log CFU/mL)/hr, respectively; methylation percentage increased with increasing soil organic matter and decreasing soil pore size. We also found significant microbial Fe(III) reduction and high levels of Fe(II) (48 %−100 % of total soluble Fe) may promote the capacity of As methylation. Although no statistical change in Fe phases was observed with low Fe dissolution and high molar Fe/As ratios, higher As bioaccessibility of colon phase (avg. 29.4 %) was mainly contributed from reductive dissolution of As(V)-bearing Fe(III) (oxy)hydroxides. Our results suggest that As mobility and biotransformation by human gut microbiota (carrying arrA and arsC genes) are strongly controlled by microbial Fe(III) reduction coupled with soil particle size. This will expand our knowledge on oral bioavailability of soil As and health risks from exposure to contaminated soils. |
| first_indexed | 2024-04-09T16:07:44Z |
| format | Article |
| id | doaj.art-9bb048f7f5b84f778b6c599c2658715a |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-04-09T16:07:44Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-9bb048f7f5b84f778b6c599c2658715a2023-04-25T04:07:45ZengElsevierEnvironment International0160-41202023-04-01174107911Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tractNaiyi Yin0Xuhui Chang1Peng Xiao2Yi Zhou3Xiaotong Liu4Shimao Xiong5Pengfei Wang6Xiaolin Cai7Guoxin Sun8Yanshan Cui9Zhengyi Hu10College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of ChinaResearch Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of China; Corresponding author at: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of China.College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of China; Corresponding authors at: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China.College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of China; Sino-Danish Centre for Education and Research, University of Chinese Academy of Sciences, Beijing 101408, People’s Republic of ChinaGut microbiota provides protection against arsenic (As) induced toxicity, and As metabolism is considered an important part of risk assessment associated with soil As exposures. However, little is known about microbial iron(III) reduction and its role in metabolism of soil-bound As in the human gut. Here, we determined the dissolution and transformation of As and Fe from incidental ingestion of contaminated soils as a function of particle size (<250 μm, 100–250 μm, 50–100 μm and < 50 μm). Colon incubation with human gut microbiota yielded a high degree of As reduction and methylation of up to 53.4 and 0.074 μg/(log CFU/mL)/hr, respectively; methylation percentage increased with increasing soil organic matter and decreasing soil pore size. We also found significant microbial Fe(III) reduction and high levels of Fe(II) (48 %−100 % of total soluble Fe) may promote the capacity of As methylation. Although no statistical change in Fe phases was observed with low Fe dissolution and high molar Fe/As ratios, higher As bioaccessibility of colon phase (avg. 29.4 %) was mainly contributed from reductive dissolution of As(V)-bearing Fe(III) (oxy)hydroxides. Our results suggest that As mobility and biotransformation by human gut microbiota (carrying arrA and arsC genes) are strongly controlled by microbial Fe(III) reduction coupled with soil particle size. This will expand our knowledge on oral bioavailability of soil As and health risks from exposure to contaminated soils.http://www.sciencedirect.com/science/article/pii/S0160412023001848ArsenicIronGut microbiomtaMetabolismSpeciation analysisSimulator of the Human Intestinal Microbial Ecosystem |
| spellingShingle | Naiyi Yin Xuhui Chang Peng Xiao Yi Zhou Xiaotong Liu Shimao Xiong Pengfei Wang Xiaolin Cai Guoxin Sun Yanshan Cui Zhengyi Hu Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract Environment International Arsenic Iron Gut microbiomta Metabolism Speciation analysis Simulator of the Human Intestinal Microbial Ecosystem |
| title | Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract |
| title_full | Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract |
| title_fullStr | Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract |
| title_full_unstemmed | Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract |
| title_short | Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract |
| title_sort | role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract |
| topic | Arsenic Iron Gut microbiomta Metabolism Speciation analysis Simulator of the Human Intestinal Microbial Ecosystem |
| url | http://www.sciencedirect.com/science/article/pii/S0160412023001848 |
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