Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda
To investigate the function of the cell cycle Cyclin E gene (ccne) in the ovarian development of Exopalaemon carinicauda, the gene was cloned using the RACE technique, and the relative expression of ccne was analyzed in different tissues at different stages of ovarian development. The effect of knoc...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002557 |
| _version_ | 1797676635163459584 |
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| author | Simian Zhang Zhiyu Pang Jiayi Gao Qin Dai Xue Liu Yuxin Shen Wazir Ali Baloch Sumera Noonari Panpan Wang Huan Gao |
| author_facet | Simian Zhang Zhiyu Pang Jiayi Gao Qin Dai Xue Liu Yuxin Shen Wazir Ali Baloch Sumera Noonari Panpan Wang Huan Gao |
| author_sort | Simian Zhang |
| collection | DOAJ |
| description | To investigate the function of the cell cycle Cyclin E gene (ccne) in the ovarian development of Exopalaemon carinicauda, the gene was cloned using the RACE technique, and the relative expression of ccne was analyzed in different tissues at different stages of ovarian development. The effect of knocking down the expression of ccne on yolk protein vitellogenin (vg) was also investigated at ovarian developmental stage II using RNAi. The results showed that the full-length cDNA sequence of ccne in E. carinicauda (1692 bp, with a 5′UTR of 127 bp, a 3′UTR of 299 bp, and an ORF of 1266 bp) could encode a total of 421 amino acids, with a predicted protein molecular weight of 48.04 kDa and a theoretical isoelectric point of 6.28. The relative expression of ccne was high during all stages of ovarian development, with the highest expression occurring in stage III. In addition, the phylogenetic tree showed that E. carinicauda and Penaeus monodon were clustered into one branch. Knocking down the ccne expression inhibited the expression of vg, meaning that the expression of ccne and vg were positively correlated. In situ hybridization showed that ccne was abundantly expressed in ova, thus inferring that ccne plays a regulatory role and is involved in nutrient accumulation and transport in the ovaries of E. carinicauda. |
| first_indexed | 2024-03-11T22:32:05Z |
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| id | doaj.art-9bb42b9e28974f0b9a118f1ab1e37661 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-11T22:32:05Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-9bb42b9e28974f0b9a118f1ab1e376612023-09-23T05:11:26ZengElsevierAquaculture Reports2352-51342023-10-0132101716Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicaudaSimian Zhang0Zhiyu Pang1Jiayi Gao2Qin Dai3Xue Liu4Yuxin Shen5Wazir Ali Baloch6Sumera Noonari7Panpan Wang8Huan Gao9Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, ChinaDepartment of Freshwater Biology and Fisheries, University of Sindh Jamshoro, 76080, PakistanDepartment of Freshwater Biology and Fisheries, University of Sindh Jamshoro, 76080, PakistanJiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, Jiangsu, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, Jiangsu, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, Jiangsu, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, Jiangsu, China; Corresponding author at: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China.To investigate the function of the cell cycle Cyclin E gene (ccne) in the ovarian development of Exopalaemon carinicauda, the gene was cloned using the RACE technique, and the relative expression of ccne was analyzed in different tissues at different stages of ovarian development. The effect of knocking down the expression of ccne on yolk protein vitellogenin (vg) was also investigated at ovarian developmental stage II using RNAi. The results showed that the full-length cDNA sequence of ccne in E. carinicauda (1692 bp, with a 5′UTR of 127 bp, a 3′UTR of 299 bp, and an ORF of 1266 bp) could encode a total of 421 amino acids, with a predicted protein molecular weight of 48.04 kDa and a theoretical isoelectric point of 6.28. The relative expression of ccne was high during all stages of ovarian development, with the highest expression occurring in stage III. In addition, the phylogenetic tree showed that E. carinicauda and Penaeus monodon were clustered into one branch. Knocking down the ccne expression inhibited the expression of vg, meaning that the expression of ccne and vg were positively correlated. In situ hybridization showed that ccne was abundantly expressed in ova, thus inferring that ccne plays a regulatory role and is involved in nutrient accumulation and transport in the ovaries of E. carinicauda.http://www.sciencedirect.com/science/article/pii/S2352513423002557Exopalaemon carinicaudaCyclin EVitellogenin geneGonadal developmentIn situ hybridization |
| spellingShingle | Simian Zhang Zhiyu Pang Jiayi Gao Qin Dai Xue Liu Yuxin Shen Wazir Ali Baloch Sumera Noonari Panpan Wang Huan Gao Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda Aquaculture Reports Exopalaemon carinicauda Cyclin E Vitellogenin gene Gonadal development In situ hybridization |
| title | Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda |
| title_full | Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda |
| title_fullStr | Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda |
| title_full_unstemmed | Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda |
| title_short | Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda |
| title_sort | functional analysis of the cell cycle protein e gene ccne in ovarian development of the white ridgetail prawn exopalaemon carinicauda |
| topic | Exopalaemon carinicauda Cyclin E Vitellogenin gene Gonadal development In situ hybridization |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002557 |
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