Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead
The objective of present study was to investigate the effects of aqueous garlic extracts, vitamin E and N-acetylcysteine on lead-induced lipid peroxidation, changes in antioxidant defense system and semen quality in the rat testes. Twenty-five male rats were divided into five groups. Animals within...
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| Format: | Article |
| Language: | English |
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Urmia University
2014-06-01
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| Series: | Veterinary Research Forum |
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| Online Access: | http://www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-4%20no-4/251-257-0359.pdf |
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| author | Reza Asadpour Mehdi Azari Marzie Hejazi Hossein Tayefi Neda Zaboli |
| author_facet | Reza Asadpour Mehdi Azari Marzie Hejazi Hossein Tayefi Neda Zaboli |
| author_sort | Reza Asadpour |
| collection | DOAJ |
| description | The objective of present study was to investigate the effects of aqueous garlic extracts, vitamin E and N-acetylcysteine on lead-induced lipid peroxidation, changes in antioxidant defense system and semen quality in the rat testes. Twenty-five male rats were divided into five groups. Animals within different treatment groups were maintained on their respective diets for 35 days as follows: group 1 rats served as control and received water and standard pellets as food ad libitum; group 2 received lead acetate by gavage (1000 ppm); group 3 was treated with A. sativum extract (400 mg kg-1, by gavage) plus lead acetate (1000 ppm); group 4 was treated orally with vitamin E (300 mg of alpha-tocopherol per kg of chow) plus lead acetate (1000 ppm); group 5 was treated orally with N-acetylcysteine (800 ppm)plus lead acetate (1000 ppm). The weights of testes, epididymis, epididymal sperm count, viable and motile sperms decreased significantly (p < 0.05) in lead-exposed rats. However treatment with vitamin E and aqueous garlic extract resulted in a significant (p < 0.05) increase in sperm motility and viability. Exposure to lead acetate significantly increased malondialdehyde (MDA) level with a significant decrease in the superoxide dismutase (SOD) activities in the testes of rats while co-administration of vitamin E and lead caused a significant (p < 0.05) decrease in MDA concentration compared with lead-exposed group. These results suggest that both vitamin E and in to a lesser extent aqueous garlic extract have a potent antioxidant protection in the testes of rat against the lead-induced oxidative stress. |
| first_indexed | 2024-12-13T08:19:00Z |
| format | Article |
| id | doaj.art-9bb4cb6857524857b6857f0e6ea87711 |
| institution | Directory Open Access Journal |
| issn | 2008-8140 |
| language | English |
| last_indexed | 2024-12-13T08:19:00Z |
| publishDate | 2014-06-01 |
| publisher | Urmia University |
| record_format | Article |
| series | Veterinary Research Forum |
| spelling | doaj.art-9bb4cb6857524857b6857f0e6ea877112022-12-21T23:54:03ZengUrmia UniversityVeterinary Research Forum2008-81402014-06-0144251257Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to leadReza Asadpour0Mehdi Azari1Marzie Hejazi2Hossein Tayefi3Neda Zaboli4Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranDepartment of Basic science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranDepartment of Basic science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranDepartment of Basic science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranDepartment of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranThe objective of present study was to investigate the effects of aqueous garlic extracts, vitamin E and N-acetylcysteine on lead-induced lipid peroxidation, changes in antioxidant defense system and semen quality in the rat testes. Twenty-five male rats were divided into five groups. Animals within different treatment groups were maintained on their respective diets for 35 days as follows: group 1 rats served as control and received water and standard pellets as food ad libitum; group 2 received lead acetate by gavage (1000 ppm); group 3 was treated with A. sativum extract (400 mg kg-1, by gavage) plus lead acetate (1000 ppm); group 4 was treated orally with vitamin E (300 mg of alpha-tocopherol per kg of chow) plus lead acetate (1000 ppm); group 5 was treated orally with N-acetylcysteine (800 ppm)plus lead acetate (1000 ppm). The weights of testes, epididymis, epididymal sperm count, viable and motile sperms decreased significantly (p < 0.05) in lead-exposed rats. However treatment with vitamin E and aqueous garlic extract resulted in a significant (p < 0.05) increase in sperm motility and viability. Exposure to lead acetate significantly increased malondialdehyde (MDA) level with a significant decrease in the superoxide dismutase (SOD) activities in the testes of rats while co-administration of vitamin E and lead caused a significant (p < 0.05) decrease in MDA concentration compared with lead-exposed group. These results suggest that both vitamin E and in to a lesser extent aqueous garlic extract have a potent antioxidant protection in the testes of rat against the lead-induced oxidative stress.http://www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-4%20no-4/251-257-0359.pdfGarlic extract; Lead; N-acetylcysteine; Rat; Vitamin E |
| spellingShingle | Reza Asadpour Mehdi Azari Marzie Hejazi Hossein Tayefi Neda Zaboli Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead Veterinary Research Forum Garlic extract; Lead; N-acetylcysteine; Rat; Vitamin E |
| title | Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead |
| title_full | Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead |
| title_fullStr | Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead |
| title_full_unstemmed | Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead |
| title_short | Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead |
| title_sort | protective effects of garlic aquous extract allium sativum vitamin e and n acetylcysteine on reproductive quality of male rats exposed to lead |
| topic | Garlic extract; Lead; N-acetylcysteine; Rat; Vitamin E |
| url | http://www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-4%20no-4/251-257-0359.pdf |
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