Soluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosis
Abstract Background Previous studies showed that soluble IL-2Rα is an important marker of cellular immune activation and might be a marker of treatment efficacy for children with brucellosis. However, data regarding adult patients with brucellosis were unknown. The aim of study was to explore the po...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Infectious Diseases of Poverty |
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| Online Access: | http://link.springer.com/article/10.1186/s40249-020-00699-y |
| _version_ | 1819229842660392960 |
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| author | Hua-Li Sun Cheng-Jie Ma Xiu-Fang Du Si-Yuan Yang Xiao Lv Hong Zhao Ling-Hang Wang Yun-Xia Tang Xing-Wang Li Rong-Meng Jiang |
| author_facet | Hua-Li Sun Cheng-Jie Ma Xiu-Fang Du Si-Yuan Yang Xiao Lv Hong Zhao Ling-Hang Wang Yun-Xia Tang Xing-Wang Li Rong-Meng Jiang |
| author_sort | Hua-Li Sun |
| collection | DOAJ |
| description | Abstract Background Previous studies showed that soluble IL-2Rα is an important marker of cellular immune activation and might be a marker of treatment efficacy for children with brucellosis. However, data regarding adult patients with brucellosis were unknown. The aim of study was to explore the potential role of serum sIL-2Rα evaluating treatment responses in adult patients with brucellosis, and T cell immune status was also examined. Methods During January 2016–April 2017, 30 patients with acute brucellosis from the Third People’s Hospital of Linfen in Shanxi Province and Beijing Di Tan Hospital, and 28 healthy controls were included in this study. Peripheral blood samples were collected before and after six weeks of antibiotic treatment. Serum sIL-2Rα levels were measured by enzyme-linked immunosorbent assay, and the percentage of Th1, Th2, Tc1, Tc2, and Tregs was detected by flow cytometry after intracellular staining for cytokines (interferon-γ and interleukin-4) and Foxp3 in T lymphocytes from peripheral blood. The obtained data were analyzed with Wilcoxon ranked sum tests for paired values, Mann-Whitney U-tests for comparisons between patients and healthy controls, and Spearman rank tests for correlation analyses. Results Serum sIL-2Rα levels were significantly higher in patients than in controls (P = 0.001). A significant decline was observed in patients after the cessation of treatment (P < 0.001) and return to normal (P > 0.05). Th1, Tc1, Th2, and Tc2 cell frequencies were higher in patients than in healthy subjects (P < 0.05), while the Th1/Th2 and Tc1/Tc2 ratios were significantly lower (P = 0.0305 and 0.0005, respectively) and returned to normal levels after treatment. In patients with acute brucellosis, serum sIL-2Rα levels were negatively correlated with the Th1/Th2 ratio (r = − 0.478, P = 0.028), Tc1/Tc2 ratio (r = − 0.677, P = 0.001), and Tc1 percentage (r = − 0.516, P = 0.017). Serum sIL-2Rα and Tc2 percentages were positively correlated (r = 0.442, P = 0.045). Conclusions Based on the correlations with Th1/Th2 and Tc1/Tc2 ratios, serum sIL-2Rα levels may reflect the immune response status. sIL-2Rα may be a marker for therapeutic efficacy in acute brucellosis. |
| first_indexed | 2024-12-23T11:19:36Z |
| format | Article |
| id | doaj.art-9bb55ec1ceac43e3a22bbfed956ce444 |
| institution | Directory Open Access Journal |
| issn | 2049-9957 |
| language | English |
| last_indexed | 2024-12-23T11:19:36Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
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| series | Infectious Diseases of Poverty |
| spelling | doaj.art-9bb55ec1ceac43e3a22bbfed956ce4442022-12-21T17:49:06ZengBMCInfectious Diseases of Poverty2049-99572020-07-01911910.1186/s40249-020-00699-ySoluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosisHua-Li Sun0Cheng-Jie Ma1Xiu-Fang Du2Si-Yuan Yang3Xiao Lv4Hong Zhao5Ling-Hang Wang6Yun-Xia Tang7Xing-Wang Li8Rong-Meng Jiang9Department of Infectious Diseases, The Affiliated Hospital of Qingdao UniversityThe Laboratory of Infectious Diseases Centre, Beijing Di Tan Hospital, Capital Medical UniversityDepartment of Infectious Diseases, the Third People HospitalThe Laboratory of Infectious Diseases Centre, Beijing Di Tan Hospital, Capital Medical UniversityDepartment of Laboratory Medicine, the Third People HospitalDepartment of Laboratory Medicine, the Third People HospitalThe Laboratory of Infectious Diseases Centre, Beijing Di Tan Hospital, Capital Medical UniversityThe Laboratory of Infectious Diseases Centre, Beijing Di Tan Hospital, Capital Medical UniversityCenter for Infectious Diseases, Beijing Di Tan Hospital, Capital Medical UniversityCenter for Infectious Diseases, Beijing Di Tan Hospital, Capital Medical UniversityAbstract Background Previous studies showed that soluble IL-2Rα is an important marker of cellular immune activation and might be a marker of treatment efficacy for children with brucellosis. However, data regarding adult patients with brucellosis were unknown. The aim of study was to explore the potential role of serum sIL-2Rα evaluating treatment responses in adult patients with brucellosis, and T cell immune status was also examined. Methods During January 2016–April 2017, 30 patients with acute brucellosis from the Third People’s Hospital of Linfen in Shanxi Province and Beijing Di Tan Hospital, and 28 healthy controls were included in this study. Peripheral blood samples were collected before and after six weeks of antibiotic treatment. Serum sIL-2Rα levels were measured by enzyme-linked immunosorbent assay, and the percentage of Th1, Th2, Tc1, Tc2, and Tregs was detected by flow cytometry after intracellular staining for cytokines (interferon-γ and interleukin-4) and Foxp3 in T lymphocytes from peripheral blood. The obtained data were analyzed with Wilcoxon ranked sum tests for paired values, Mann-Whitney U-tests for comparisons between patients and healthy controls, and Spearman rank tests for correlation analyses. Results Serum sIL-2Rα levels were significantly higher in patients than in controls (P = 0.001). A significant decline was observed in patients after the cessation of treatment (P < 0.001) and return to normal (P > 0.05). Th1, Tc1, Th2, and Tc2 cell frequencies were higher in patients than in healthy subjects (P < 0.05), while the Th1/Th2 and Tc1/Tc2 ratios were significantly lower (P = 0.0305 and 0.0005, respectively) and returned to normal levels after treatment. In patients with acute brucellosis, serum sIL-2Rα levels were negatively correlated with the Th1/Th2 ratio (r = − 0.478, P = 0.028), Tc1/Tc2 ratio (r = − 0.677, P = 0.001), and Tc1 percentage (r = − 0.516, P = 0.017). Serum sIL-2Rα and Tc2 percentages were positively correlated (r = 0.442, P = 0.045). Conclusions Based on the correlations with Th1/Th2 and Tc1/Tc2 ratios, serum sIL-2Rα levels may reflect the immune response status. sIL-2Rα may be a marker for therapeutic efficacy in acute brucellosis.http://link.springer.com/article/10.1186/s40249-020-00699-yBrucellosissIL-2RαTc1Tc2Th1Th2 |
| spellingShingle | Hua-Li Sun Cheng-Jie Ma Xiu-Fang Du Si-Yuan Yang Xiao Lv Hong Zhao Ling-Hang Wang Yun-Xia Tang Xing-Wang Li Rong-Meng Jiang Soluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosis Infectious Diseases of Poverty Brucellosis sIL-2Rα Tc1 Tc2 Th1 Th2 |
| title | Soluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosis |
| title_full | Soluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosis |
| title_fullStr | Soluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosis |
| title_full_unstemmed | Soluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosis |
| title_short | Soluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosis |
| title_sort | soluble il 2rα correlates with imbalances of th1 th2 and tc1 tc2 cells in patients with acute brucellosis |
| topic | Brucellosis sIL-2Rα Tc1 Tc2 Th1 Th2 |
| url | http://link.springer.com/article/10.1186/s40249-020-00699-y |
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