TRPC1 Regulates the Activity of a Voltage-Dependent Nonselective Cation Current in Hippocampal CA1 Neurons
The cation channel subunit TRPC1 is strongly expressed in central neurons including neurons in the CA1 region of the hippocampus where it forms complexes with TRPC4 and TRPC5. To investigate the functional role of TRPC1 in these neurons and in channel function, we compared current responses to group...
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MDPI AG
2020-02-01
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| Online Access: | https://www.mdpi.com/2073-4409/9/2/459 |
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| author | Frauke Kepura Eva Braun Alexander Dietrich Tim D. Plant |
| author_facet | Frauke Kepura Eva Braun Alexander Dietrich Tim D. Plant |
| author_sort | Frauke Kepura |
| collection | DOAJ |
| description | The cation channel subunit TRPC1 is strongly expressed in central neurons including neurons in the CA1 region of the hippocampus where it forms complexes with TRPC4 and TRPC5. To investigate the functional role of TRPC1 in these neurons and in channel function, we compared current responses to group I metabotropic glutamate receptor (mGluR I) activation and looked for major differences in dendritic morphology in neurons from <i>TRPC1<sup>+/+</sup></i> and <i>TRPC1<sup>−/−</sup></i> mice. mGluR I stimulation resulted in the activation of a voltage-dependent nonselective cation current in both genotypes. Deletion of TRPC1 resulted in a modification of the shape of the current-voltage relationship, leading to an inward current increase. In current clamp recordings, the percentage of neurons that responded to depolarization in the presence of an mGluR I agonist with a plateau potential was increased in <i>TRPC1<sup>−/−</sup></i> mice. There was also a small increase in the minor population of CA1 neurons that have more than one apical dendrite in <i>TRPC1<sup>−/−</sup></i> mice. We conclude that TRPC1 has an inhibitory effect on receptor-operated nonselective cation channels in hippocampal CA1 neurons probably as a result of heterotetramer formation with other TRPC isoforms, and that TRPC1 deletion has only minor effects on dendritic morphology. |
| first_indexed | 2024-03-12T20:18:41Z |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T20:18:41Z |
| publishDate | 2020-02-01 |
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| spelling | doaj.art-9bb5e8213455441283e22b1c1930eabb2023-08-02T01:09:52ZengMDPI AGCells2073-44092020-02-019245910.3390/cells9020459cells9020459TRPC1 Regulates the Activity of a Voltage-Dependent Nonselective Cation Current in Hippocampal CA1 NeuronsFrauke Kepura0Eva Braun1Alexander Dietrich2Tim D. Plant3Pharmakologisches Institut, BPC-Marburg, Fachbereich Medizin, Philipps-Universität Marburg, Karl-von-Frisch-Straße 2, 35043 Marburg, GermanyPharmakologisches Institut, BPC-Marburg, Fachbereich Medizin, Philipps-Universität Marburg, Karl-von-Frisch-Straße 2, 35043 Marburg, GermanyPharmakologisches Institut, BPC-Marburg, Fachbereich Medizin, Philipps-Universität Marburg, Karl-von-Frisch-Straße 2, 35043 Marburg, GermanyPharmakologisches Institut, BPC-Marburg, Fachbereich Medizin, Philipps-Universität Marburg, Karl-von-Frisch-Straße 2, 35043 Marburg, GermanyThe cation channel subunit TRPC1 is strongly expressed in central neurons including neurons in the CA1 region of the hippocampus where it forms complexes with TRPC4 and TRPC5. To investigate the functional role of TRPC1 in these neurons and in channel function, we compared current responses to group I metabotropic glutamate receptor (mGluR I) activation and looked for major differences in dendritic morphology in neurons from <i>TRPC1<sup>+/+</sup></i> and <i>TRPC1<sup>−/−</sup></i> mice. mGluR I stimulation resulted in the activation of a voltage-dependent nonselective cation current in both genotypes. Deletion of TRPC1 resulted in a modification of the shape of the current-voltage relationship, leading to an inward current increase. In current clamp recordings, the percentage of neurons that responded to depolarization in the presence of an mGluR I agonist with a plateau potential was increased in <i>TRPC1<sup>−/−</sup></i> mice. There was also a small increase in the minor population of CA1 neurons that have more than one apical dendrite in <i>TRPC1<sup>−/−</sup></i> mice. We conclude that TRPC1 has an inhibitory effect on receptor-operated nonselective cation channels in hippocampal CA1 neurons probably as a result of heterotetramer formation with other TRPC isoforms, and that TRPC1 deletion has only minor effects on dendritic morphology.https://www.mdpi.com/2073-4409/9/2/459trpc channelscation channelstrpc channel regulationmolecular physiologytrpc1nonselective cation currenthippocampusca1 |
| spellingShingle | Frauke Kepura Eva Braun Alexander Dietrich Tim D. Plant TRPC1 Regulates the Activity of a Voltage-Dependent Nonselective Cation Current in Hippocampal CA1 Neurons Cells trpc channels cation channels trpc channel regulation molecular physiology trpc1 nonselective cation current hippocampus ca1 |
| title | TRPC1 Regulates the Activity of a Voltage-Dependent Nonselective Cation Current in Hippocampal CA1 Neurons |
| title_full | TRPC1 Regulates the Activity of a Voltage-Dependent Nonselective Cation Current in Hippocampal CA1 Neurons |
| title_fullStr | TRPC1 Regulates the Activity of a Voltage-Dependent Nonselective Cation Current in Hippocampal CA1 Neurons |
| title_full_unstemmed | TRPC1 Regulates the Activity of a Voltage-Dependent Nonselective Cation Current in Hippocampal CA1 Neurons |
| title_short | TRPC1 Regulates the Activity of a Voltage-Dependent Nonselective Cation Current in Hippocampal CA1 Neurons |
| title_sort | trpc1 regulates the activity of a voltage dependent nonselective cation current in hippocampal ca1 neurons |
| topic | trpc channels cation channels trpc channel regulation molecular physiology trpc1 nonselective cation current hippocampus ca1 |
| url | https://www.mdpi.com/2073-4409/9/2/459 |
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