<i>PpMYB39</i> Activates <i>PpDFR</i> to Modulate Anthocyanin Biosynthesis during Peach Fruit Maturation
Anthocyanins are a class of water-soluble flavonoids widely present in fruits and vegetables responsible for the red flesh formation of peach fruit. Previously, several genes of the MYB family have been reported as transcriptional regulators of the anthocyanin biosynthetic pathway of structural gene...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/4/332 |
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| author | Irshad Ahmad Khan Masood Ur Rahman Shazia Sakhi Ghazala Nawaz Aftab Ahmad Khan Tanveer Ahmad Mohammad Adnan Shah Masaud Khan |
| author_facet | Irshad Ahmad Khan Masood Ur Rahman Shazia Sakhi Ghazala Nawaz Aftab Ahmad Khan Tanveer Ahmad Mohammad Adnan Shah Masaud Khan |
| author_sort | Irshad Ahmad Khan |
| collection | DOAJ |
| description | Anthocyanins are a class of water-soluble flavonoids widely present in fruits and vegetables responsible for the red flesh formation of peach fruit. Previously, several genes of the MYB family have been reported as transcriptional regulators of the anthocyanin biosynthetic pathway of structural genes in plants. In this study, through comparative transcriptome analysis of the white and red flesh peach cultivars of Harrow Blood and Asama Hakuto, a predicted transcription factor of the R2R3MYB family, <i>PpMYB39</i>, was identified to be associated with anthocyanin biosynthesis in peach fruit. In red-fleshed peach cultivars, the maximum amount of anthocyanin accumulated 95 days after full bloom (DAFB), at full maturity near ripening. Our results showed that, at this stage, <i>PpMYB39</i> had the highest expression level among the 13 differentially expressed genes (DEGs) found in both red- and white-fleshed fruits, as well as a high correlation with total anthocyanin content throughout fruit development. Moreover, the expression analysis of the structural genes of the anthocyanin biosynthetic pathway in peach fruit revealed that <i>Prunus persica</i> Dihydroflavonol-4-reductase (<i>PpDFR</i>) was co-expressed and up-regulated with <i>PpMYB39</i> at 95 DAFB, suggesting its possible role as a transcriptional activator of <i>MYB39</i>. This was further confirmed by a yeast one-hybrid assay and a dual luciferase reporter assay. Our results will be helpful in the breeding of peach cultivars and the identification and significance of color in peaches and related fruit species, in addition to providing an understanding of color formation in peach fruit for future research. |
| first_indexed | 2024-03-09T13:35:13Z |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T13:35:13Z |
| publishDate | 2022-04-01 |
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| series | Horticulturae |
| spelling | doaj.art-9bb749593eb54301bf4fb5534de936282023-11-30T21:13:02ZengMDPI AGHorticulturae2311-75242022-04-018433210.3390/horticulturae8040332<i>PpMYB39</i> Activates <i>PpDFR</i> to Modulate Anthocyanin Biosynthesis during Peach Fruit MaturationIrshad Ahmad Khan0Masood Ur Rahman1Shazia Sakhi2Ghazala Nawaz3Aftab Ahmad Khan4Tanveer Ahmad5Mohammad Adnan6Shah Masaud Khan7Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaCentre for Plant Science and Biodiversity, Faculty of Life Sciences, University of Swat, Mingora 19200, PakistanCentre for Plant Science and Biodiversity, Faculty of Life Sciences, University of Swat, Mingora 19200, PakistanDepartment of Botany, Kohat University of Science and Technology, Kohat 26000, PakistanAgriculture Research Institute Swat, Agriculture, Livestock and Cooperative Department, Government of Khyber Pakhtunkhwa, Mingora 19130, PakistanCentre for Plant Science and Biodiversity, Faculty of Life Sciences, University of Swat, Mingora 19200, PakistanCentre for Plant Science and Biodiversity, Faculty of Life Sciences, University of Swat, Mingora 19200, PakistanDepartment of Horticulture, Faculty of Agricultural Sciences, The University of Haripur, Haripur 22620, PakistanAnthocyanins are a class of water-soluble flavonoids widely present in fruits and vegetables responsible for the red flesh formation of peach fruit. Previously, several genes of the MYB family have been reported as transcriptional regulators of the anthocyanin biosynthetic pathway of structural genes in plants. In this study, through comparative transcriptome analysis of the white and red flesh peach cultivars of Harrow Blood and Asama Hakuto, a predicted transcription factor of the R2R3MYB family, <i>PpMYB39</i>, was identified to be associated with anthocyanin biosynthesis in peach fruit. In red-fleshed peach cultivars, the maximum amount of anthocyanin accumulated 95 days after full bloom (DAFB), at full maturity near ripening. Our results showed that, at this stage, <i>PpMYB39</i> had the highest expression level among the 13 differentially expressed genes (DEGs) found in both red- and white-fleshed fruits, as well as a high correlation with total anthocyanin content throughout fruit development. Moreover, the expression analysis of the structural genes of the anthocyanin biosynthetic pathway in peach fruit revealed that <i>Prunus persica</i> Dihydroflavonol-4-reductase (<i>PpDFR</i>) was co-expressed and up-regulated with <i>PpMYB39</i> at 95 DAFB, suggesting its possible role as a transcriptional activator of <i>MYB39</i>. This was further confirmed by a yeast one-hybrid assay and a dual luciferase reporter assay. Our results will be helpful in the breeding of peach cultivars and the identification and significance of color in peaches and related fruit species, in addition to providing an understanding of color formation in peach fruit for future research.https://www.mdpi.com/2311-7524/8/4/332peachflesh coloranthocyanintranscriptome analysisMYB |
| spellingShingle | Irshad Ahmad Khan Masood Ur Rahman Shazia Sakhi Ghazala Nawaz Aftab Ahmad Khan Tanveer Ahmad Mohammad Adnan Shah Masaud Khan <i>PpMYB39</i> Activates <i>PpDFR</i> to Modulate Anthocyanin Biosynthesis during Peach Fruit Maturation Horticulturae peach flesh color anthocyanin transcriptome analysis MYB |
| title | <i>PpMYB39</i> Activates <i>PpDFR</i> to Modulate Anthocyanin Biosynthesis during Peach Fruit Maturation |
| title_full | <i>PpMYB39</i> Activates <i>PpDFR</i> to Modulate Anthocyanin Biosynthesis during Peach Fruit Maturation |
| title_fullStr | <i>PpMYB39</i> Activates <i>PpDFR</i> to Modulate Anthocyanin Biosynthesis during Peach Fruit Maturation |
| title_full_unstemmed | <i>PpMYB39</i> Activates <i>PpDFR</i> to Modulate Anthocyanin Biosynthesis during Peach Fruit Maturation |
| title_short | <i>PpMYB39</i> Activates <i>PpDFR</i> to Modulate Anthocyanin Biosynthesis during Peach Fruit Maturation |
| title_sort | i ppmyb39 i activates i ppdfr i to modulate anthocyanin biosynthesis during peach fruit maturation |
| topic | peach flesh color anthocyanin transcriptome analysis MYB |
| url | https://www.mdpi.com/2311-7524/8/4/332 |
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