Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
In this work, we reported a systematic investigation on the interactions betweenmethylene blue (MB) and surface-confined DNA by using electrochemical methods. Wedemonstrated that the redox potential of MB and binding and dissociation kinetics of MB toDNA differed significantly for single-stranded DN...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2007-11-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/7/11/2671/ |
| Summary: | In this work, we reported a systematic investigation on the interactions betweenmethylene blue (MB) and surface-confined DNA by using electrochemical methods. Wedemonstrated that the redox potential of MB and binding and dissociation kinetics of MB toDNA differed significantly for single-stranded DNA (ss-DNA) and double-stranded DNA(ds-DNA) immobilized on gold electrodes. This was possibly due to the different bindingmechanism between MB and ss- or ds-DNA. This work might provide useful informationfor developing MB-based sequence-specific electrochemical DNA sensors. |
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| ISSN: | 1424-8220 |