Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
In this work, we reported a systematic investigation on the interactions betweenmethylene blue (MB) and surface-confined DNA by using electrochemical methods. Wedemonstrated that the redox potential of MB and binding and dissociation kinetics of MB toDNA differed significantly for single-stranded DN...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2007-11-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/7/11/2671/ |
| _version_ | 1818036972733595648 |
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| author | Chunhai Fan Shiping Song Jiong Zhang Lihua Wang Ying Wan Xiaolei Zuo Dun Pan |
| author_facet | Chunhai Fan Shiping Song Jiong Zhang Lihua Wang Ying Wan Xiaolei Zuo Dun Pan |
| author_sort | Chunhai Fan |
| collection | DOAJ |
| description | In this work, we reported a systematic investigation on the interactions betweenmethylene blue (MB) and surface-confined DNA by using electrochemical methods. Wedemonstrated that the redox potential of MB and binding and dissociation kinetics of MB toDNA differed significantly for single-stranded DNA (ss-DNA) and double-stranded DNA(ds-DNA) immobilized on gold electrodes. This was possibly due to the different bindingmechanism between MB and ss- or ds-DNA. This work might provide useful informationfor developing MB-based sequence-specific electrochemical DNA sensors. |
| first_indexed | 2024-12-10T07:19:27Z |
| format | Article |
| id | doaj.art-9bb7e75df0484ecb9d7007007928dcee |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T07:19:27Z |
| publishDate | 2007-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9bb7e75df0484ecb9d7007007928dcee2022-12-22T01:57:51ZengMDPI AGSensors1424-82202007-11-017112671268010.3390/s7112671Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene BlueChunhai FanShiping SongJiong ZhangLihua WangYing WanXiaolei ZuoDun PanIn this work, we reported a systematic investigation on the interactions betweenmethylene blue (MB) and surface-confined DNA by using electrochemical methods. Wedemonstrated that the redox potential of MB and binding and dissociation kinetics of MB toDNA differed significantly for single-stranded DNA (ss-DNA) and double-stranded DNA(ds-DNA) immobilized on gold electrodes. This was possibly due to the different bindingmechanism between MB and ss- or ds-DNA. This work might provide useful informationfor developing MB-based sequence-specific electrochemical DNA sensors.http://www.mdpi.com/1424-8220/7/11/2671/Electrochemical DNA sensorsmethylene blueelectrochemical indicators. |
| spellingShingle | Chunhai Fan Shiping Song Jiong Zhang Lihua Wang Ying Wan Xiaolei Zuo Dun Pan Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue Sensors Electrochemical DNA sensors methylene blue electrochemical indicators. |
| title | Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue |
| title_full | Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue |
| title_fullStr | Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue |
| title_full_unstemmed | Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue |
| title_short | Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue |
| title_sort | electrochemical interrogation of interactions between surface confined dna and methylene blue |
| topic | Electrochemical DNA sensors methylene blue electrochemical indicators. |
| url | http://www.mdpi.com/1424-8220/7/11/2671/ |
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