Identification of a Plum pox virus CI-Interacting Protein from Chloroplast That Has a Negative Effect in Virus Infection
The cylindrical inclusion (CI) protein of potyviruses is involved in virus replication and cell-to-cell movement. These two processes should rely on multiple plant-virus interactions; however, little is known about the host factors that are involved in, or that may interfere with, CI functions. By u...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2006-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-0350 |
| _version_ | 1811291396264427520 |
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| author | I. Jiménez L. López J. M. Alamillo A. Valli J. A. García |
| author_facet | I. Jiménez L. López J. M. Alamillo A. Valli J. A. García |
| author_sort | I. Jiménez |
| collection | DOAJ |
| description | The cylindrical inclusion (CI) protein of potyviruses is involved in virus replication and cell-to-cell movement. These two processes should rely on multiple plant-virus interactions; however, little is known about the host factors that are involved in, or that may interfere with, CI functions. By using a yeast two-hybrid system, the CI protein from Plum pox virus (PPV) was found to interact with the photosystem I PSI-K protein, the product of the gene psaK, of Nicotiana benthamiana. Coexpression of PPV CI was shown to cause a decrease in the accumulation level of PSI-K transiently expressed in N. benthamiana leaves. To test the biological relevance of this interaction, we have analyzed the infection of PPV in N. benthamiana plants in which psaK gene expression has been silenced by RNA interference, as well as in Arabidopsis thaliana psaK knockout plants. Our results show that downregulation of the psaK gene leads to higher PPV accumulation, suggesting a role for the CI-PSI-K interaction in PPV infection. |
| first_indexed | 2024-04-13T04:28:13Z |
| format | Article |
| id | doaj.art-9bb8436d1eb14db2975790e41f68e131 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:28:13Z |
| publishDate | 2006-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-9bb8436d1eb14db2975790e41f68e1312022-12-22T03:02:25ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062006-03-0119335035810.1094/MPMI-19-0350Identification of a Plum pox virus CI-Interacting Protein from Chloroplast That Has a Negative Effect in Virus InfectionI. JiménezL. LópezJ. M. AlamilloA. ValliJ. A. GarcíaThe cylindrical inclusion (CI) protein of potyviruses is involved in virus replication and cell-to-cell movement. These two processes should rely on multiple plant-virus interactions; however, little is known about the host factors that are involved in, or that may interfere with, CI functions. By using a yeast two-hybrid system, the CI protein from Plum pox virus (PPV) was found to interact with the photosystem I PSI-K protein, the product of the gene psaK, of Nicotiana benthamiana. Coexpression of PPV CI was shown to cause a decrease in the accumulation level of PSI-K transiently expressed in N. benthamiana leaves. To test the biological relevance of this interaction, we have analyzed the infection of PPV in N. benthamiana plants in which psaK gene expression has been silenced by RNA interference, as well as in Arabidopsis thaliana psaK knockout plants. Our results show that downregulation of the psaK gene leads to higher PPV accumulation, suggesting a role for the CI-PSI-K interaction in PPV infection.https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-0350host proteinsRNA helicaseRNA silencingsharka diseaseviral proteins |
| spellingShingle | I. Jiménez L. López J. M. Alamillo A. Valli J. A. García Identification of a Plum pox virus CI-Interacting Protein from Chloroplast That Has a Negative Effect in Virus Infection Molecular Plant-Microbe Interactions host proteins RNA helicase RNA silencing sharka disease viral proteins |
| title | Identification of a Plum pox virus CI-Interacting Protein from Chloroplast That Has a Negative Effect in Virus Infection |
| title_full | Identification of a Plum pox virus CI-Interacting Protein from Chloroplast That Has a Negative Effect in Virus Infection |
| title_fullStr | Identification of a Plum pox virus CI-Interacting Protein from Chloroplast That Has a Negative Effect in Virus Infection |
| title_full_unstemmed | Identification of a Plum pox virus CI-Interacting Protein from Chloroplast That Has a Negative Effect in Virus Infection |
| title_short | Identification of a Plum pox virus CI-Interacting Protein from Chloroplast That Has a Negative Effect in Virus Infection |
| title_sort | identification of a plum pox virus ci interacting protein from chloroplast that has a negative effect in virus infection |
| topic | host proteins RNA helicase RNA silencing sharka disease viral proteins |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-0350 |
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