Automated DNA Extraction Monitoring System Based on MTConnect Technology

MTConnect standard technology provides simplicity, flexibility, and scalability in integrating various equipment and operating systems and enabling accurate and consistent data collection from any MTConnect-compatible system. Using MTConnect technology, it is possible to immediately identify the cau...

Full description

Bibliographic Details
Main Authors: Sang-Ho Han, Ae-Ja Park, Ah-Reum Park, Mun-Ho Ryu
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/11/2/684
_version_ 1797412531044614144
author Sang-Ho Han
Ae-Ja Park
Ah-Reum Park
Mun-Ho Ryu
author_facet Sang-Ho Han
Ae-Ja Park
Ah-Reum Park
Mun-Ho Ryu
author_sort Sang-Ho Han
collection DOAJ
description MTConnect standard technology provides simplicity, flexibility, and scalability in integrating various equipment and operating systems and enabling accurate and consistent data collection from any MTConnect-compatible system. Using MTConnect technology, it is possible to immediately identify the cause of a problem and respond quickly when a problem occurs. Molecular genetic diagnostic point-of-care testing (POCT) devices have received attention in recent years because they enable rapid disease diagnosis. A molecular genetic diagnostic POCT device is under development by the authors. The system consists of a gene extraction process and a real-time PCR-based gene amplification process. In this study, we propose and demonstrate a system based on MTConnect technology to monitor an automated DNA extraction process. The proposed system consists of an automated DNA extraction system, an MTConnect adapter, an MTConnect agent, and a client application. The adapter and agent were developed on a Raspberry Pi single-board computer. The agent publishes the collected data in Extensible Markup Language (XML) format over a network. The performance and reliability of the system were evaluated by verifying the request response time between the implemented system’s agent and the client application. The results demonstrate the feasibility of monitoring the DNA extraction process over a network.
first_indexed 2024-03-09T05:04:15Z
format Article
id doaj.art-9bb871f938274e1abd3a71e249279421
institution Directory Open Access Journal
issn 2076-3417
language English
last_indexed 2024-03-09T05:04:15Z
publishDate 2021-01-01
publisher MDPI AG
record_format Article
series Applied Sciences
spelling doaj.art-9bb871f938274e1abd3a71e2492794212023-12-03T12:56:53ZengMDPI AGApplied Sciences2076-34172021-01-0111268410.3390/app11020684Automated DNA Extraction Monitoring System Based on MTConnect TechnologySang-Ho Han0Ae-Ja Park1Ah-Reum Park2Mun-Ho Ryu3Department of Healthcare Engineering, Jeonbuk National University, Jeonju 54896, KoreaDepartment of Bioinstrument, Biomedux Co., Suwon 16226, KoreaDepartment of Bioinstrument, Biomedux Co., Suwon 16226, KoreaDivision of Biomedical Engineering, Jeonbuk National University, Jeonju 54896, KoreaMTConnect standard technology provides simplicity, flexibility, and scalability in integrating various equipment and operating systems and enabling accurate and consistent data collection from any MTConnect-compatible system. Using MTConnect technology, it is possible to immediately identify the cause of a problem and respond quickly when a problem occurs. Molecular genetic diagnostic point-of-care testing (POCT) devices have received attention in recent years because they enable rapid disease diagnosis. A molecular genetic diagnostic POCT device is under development by the authors. The system consists of a gene extraction process and a real-time PCR-based gene amplification process. In this study, we propose and demonstrate a system based on MTConnect technology to monitor an automated DNA extraction process. The proposed system consists of an automated DNA extraction system, an MTConnect adapter, an MTConnect agent, and a client application. The adapter and agent were developed on a Raspberry Pi single-board computer. The agent publishes the collected data in Extensible Markup Language (XML) format over a network. The performance and reliability of the system were evaluated by verifying the request response time between the implemented system’s agent and the client application. The results demonstrate the feasibility of monitoring the DNA extraction process over a network.https://www.mdpi.com/2076-3417/11/2/684molecular genetic testDNA extractionPOCT deviceMTConnectreal-time monitoring
spellingShingle Sang-Ho Han
Ae-Ja Park
Ah-Reum Park
Mun-Ho Ryu
Automated DNA Extraction Monitoring System Based on MTConnect Technology
Applied Sciences
molecular genetic test
DNA extraction
POCT device
MTConnect
real-time monitoring
title Automated DNA Extraction Monitoring System Based on MTConnect Technology
title_full Automated DNA Extraction Monitoring System Based on MTConnect Technology
title_fullStr Automated DNA Extraction Monitoring System Based on MTConnect Technology
title_full_unstemmed Automated DNA Extraction Monitoring System Based on MTConnect Technology
title_short Automated DNA Extraction Monitoring System Based on MTConnect Technology
title_sort automated dna extraction monitoring system based on mtconnect technology
topic molecular genetic test
DNA extraction
POCT device
MTConnect
real-time monitoring
url https://www.mdpi.com/2076-3417/11/2/684
work_keys_str_mv AT sanghohan automateddnaextractionmonitoringsystembasedonmtconnecttechnology
AT aejapark automateddnaextractionmonitoringsystembasedonmtconnecttechnology
AT ahreumpark automateddnaextractionmonitoringsystembasedonmtconnecttechnology
AT munhoryu automateddnaextractionmonitoringsystembasedonmtconnecttechnology