Assessment of the genetic relationship between <it>Dictyocaulus</it> species from <it>Bos taurus</it> and <it>Cervus elaphus</it> using complete mitochondrial genomic datasets
<p>Abstract</p> <p>Background</p> <p><it>Dictyocaulus</it> species are strongylid nematodes of major veterinary significance in ruminants, such as cattle and cervids, and cause serious bronchitis or pneumonia (dictyocaulosis or “husk”). There has been ongoin...
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://www.parasitesandvectors.com/content/5/1/241 |
| _version_ | 1819037767648149504 |
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| author | Gasser Robin B Jabbar Abdul Mohandas Namitha Höglund Johan Hall Ross S Littlewood D Timothy J Jex Aaron R |
| author_facet | Gasser Robin B Jabbar Abdul Mohandas Namitha Höglund Johan Hall Ross S Littlewood D Timothy J Jex Aaron R |
| author_sort | Gasser Robin B |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Dictyocaulus</it> species are strongylid nematodes of major veterinary significance in ruminants, such as cattle and cervids, and cause serious bronchitis or pneumonia (dictyocaulosis or “husk”). There has been ongoing controversy surrounding the validity of some <it>Dictyocaulus</it> species and their host specificity. Here, we sequenced and characterized the mitochondrial (mt) genomes of <it>Dictyocaulus viviparus</it> (from <it>Bos taurus</it>) with <it>Dictyocaulus</it> sp. cf. <it>eckerti</it> from red deer (<it>Cervus elaphus</it>), used mt datasets to assess the genetic relationship between these and related parasites, and predicted markers for future population genetic or molecular epidemiological studies.</p> <p>Methods</p> <p>The mt genomes were amplified from single adult males of <it>D. viviparus</it> and <it>Dictyocaulus</it> sp. cf. <it>eckerti</it> (from red deer) by long-PCR, sequenced using 454-technology and annotated using bioinformatic tools. Amino acid sequences inferred from individual genes of each of the two mt genomes were compared, concatenated and subjected to phylogenetic analysis using Bayesian inference (BI), also employing data for other strongylids for comparative purposes.</p> <p>Results</p> <p>The circular mt genomes were 13,310 bp (<it>D. viviparus</it>) and 13,296 bp (<it>Dictyocaulus</it> sp. cf. <it>eckerti</it>) in size, and each contained 12 protein-encoding, 22 transfer RNA and 2 ribosomal RNA genes, consistent with other strongylid nematodes sequenced to date. Sliding window analysis identified genes with high or low levels of nucleotide diversity between the mt genomes. At the predicted mt proteomic level, there was an overall sequence difference of 34.5% between <it>D. viviparus</it> and <it>Dictyocaulus</it> sp. cf. <it>eckerti</it>, and amino acid sequence variation within each species was usually much lower than differences between species. Phylogenetic analysis of the concatenated amino acid sequence data for all 12 mt proteins showed that both <it>D. viviparus</it> and <it>Dictyocaulus</it> sp. cf. <it>eckerti</it> were closely related, and grouped to the exclusion of selected members of the superfamilies Metastrongyloidea, Trichostrongyloidea, Ancylostomatoidea and Strongyloidea.</p> <p>Conclusions</p> <p>Consistent with previous findings for nuclear ribosomal DNA sequence data, the present analyses indicate that <it>Dictyocaulus</it> sp. cf. <it>eckerti</it> (red deer) and <it>D. viviparus</it> are separate species. Barcodes in the two mt genomes and proteomes should serve as markers for future studies of the population genetics and/or epidemiology of these and related species of <it>Dictyocaulus</it>.</p> |
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| spelling | doaj.art-9bb9601925a24ad4b3ff6904bd64d5f52022-12-21T19:10:18ZengBMCParasites & Vectors1756-33052012-10-015124110.1186/1756-3305-5-241Assessment of the genetic relationship between <it>Dictyocaulus</it> species from <it>Bos taurus</it> and <it>Cervus elaphus</it> using complete mitochondrial genomic datasetsGasser Robin BJabbar AbdulMohandas NamithaHöglund JohanHall Ross SLittlewood D Timothy JJex Aaron R<p>Abstract</p> <p>Background</p> <p><it>Dictyocaulus</it> species are strongylid nematodes of major veterinary significance in ruminants, such as cattle and cervids, and cause serious bronchitis or pneumonia (dictyocaulosis or “husk”). There has been ongoing controversy surrounding the validity of some <it>Dictyocaulus</it> species and their host specificity. Here, we sequenced and characterized the mitochondrial (mt) genomes of <it>Dictyocaulus viviparus</it> (from <it>Bos taurus</it>) with <it>Dictyocaulus</it> sp. cf. <it>eckerti</it> from red deer (<it>Cervus elaphus</it>), used mt datasets to assess the genetic relationship between these and related parasites, and predicted markers for future population genetic or molecular epidemiological studies.</p> <p>Methods</p> <p>The mt genomes were amplified from single adult males of <it>D. viviparus</it> and <it>Dictyocaulus</it> sp. cf. <it>eckerti</it> (from red deer) by long-PCR, sequenced using 454-technology and annotated using bioinformatic tools. Amino acid sequences inferred from individual genes of each of the two mt genomes were compared, concatenated and subjected to phylogenetic analysis using Bayesian inference (BI), also employing data for other strongylids for comparative purposes.</p> <p>Results</p> <p>The circular mt genomes were 13,310 bp (<it>D. viviparus</it>) and 13,296 bp (<it>Dictyocaulus</it> sp. cf. <it>eckerti</it>) in size, and each contained 12 protein-encoding, 22 transfer RNA and 2 ribosomal RNA genes, consistent with other strongylid nematodes sequenced to date. Sliding window analysis identified genes with high or low levels of nucleotide diversity between the mt genomes. At the predicted mt proteomic level, there was an overall sequence difference of 34.5% between <it>D. viviparus</it> and <it>Dictyocaulus</it> sp. cf. <it>eckerti</it>, and amino acid sequence variation within each species was usually much lower than differences between species. Phylogenetic analysis of the concatenated amino acid sequence data for all 12 mt proteins showed that both <it>D. viviparus</it> and <it>Dictyocaulus</it> sp. cf. <it>eckerti</it> were closely related, and grouped to the exclusion of selected members of the superfamilies Metastrongyloidea, Trichostrongyloidea, Ancylostomatoidea and Strongyloidea.</p> <p>Conclusions</p> <p>Consistent with previous findings for nuclear ribosomal DNA sequence data, the present analyses indicate that <it>Dictyocaulus</it> sp. cf. <it>eckerti</it> (red deer) and <it>D. viviparus</it> are separate species. Barcodes in the two mt genomes and proteomes should serve as markers for future studies of the population genetics and/or epidemiology of these and related species of <it>Dictyocaulus</it>.</p>http://www.parasitesandvectors.com/content/5/1/241<it>Dictyocaulus</it> (Nematoda: Strongylida)LungwormsDictyocaulosisCattleDeerMitochondrial genomeSystematicsEpidemiology |
| spellingShingle | Gasser Robin B Jabbar Abdul Mohandas Namitha Höglund Johan Hall Ross S Littlewood D Timothy J Jex Aaron R Assessment of the genetic relationship between <it>Dictyocaulus</it> species from <it>Bos taurus</it> and <it>Cervus elaphus</it> using complete mitochondrial genomic datasets Parasites & Vectors <it>Dictyocaulus</it> (Nematoda: Strongylida) Lungworms Dictyocaulosis Cattle Deer Mitochondrial genome Systematics Epidemiology |
| title | Assessment of the genetic relationship between <it>Dictyocaulus</it> species from <it>Bos taurus</it> and <it>Cervus elaphus</it> using complete mitochondrial genomic datasets |
| title_full | Assessment of the genetic relationship between <it>Dictyocaulus</it> species from <it>Bos taurus</it> and <it>Cervus elaphus</it> using complete mitochondrial genomic datasets |
| title_fullStr | Assessment of the genetic relationship between <it>Dictyocaulus</it> species from <it>Bos taurus</it> and <it>Cervus elaphus</it> using complete mitochondrial genomic datasets |
| title_full_unstemmed | Assessment of the genetic relationship between <it>Dictyocaulus</it> species from <it>Bos taurus</it> and <it>Cervus elaphus</it> using complete mitochondrial genomic datasets |
| title_short | Assessment of the genetic relationship between <it>Dictyocaulus</it> species from <it>Bos taurus</it> and <it>Cervus elaphus</it> using complete mitochondrial genomic datasets |
| title_sort | assessment of the genetic relationship between it dictyocaulus it species from it bos taurus it and it cervus elaphus it using complete mitochondrial genomic datasets |
| topic | <it>Dictyocaulus</it> (Nematoda: Strongylida) Lungworms Dictyocaulosis Cattle Deer Mitochondrial genome Systematics Epidemiology |
| url | http://www.parasitesandvectors.com/content/5/1/241 |
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