Effects of N-acetyl-L-cysteine on Postoperative Intrabdominal Adhesion in a Large Animal Model
<br /> Objective- Mechanisms that decrease oxidative stress and enhance peritoneal fibrinolysis reduce adhesions. N-acetyl-L-cysteine (NAC) is an antioxidant whose effect on peritoneal fibrinolysis in large animal model has not been established. The aims of this study were to investigate the a...
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Iranian Veterinary Surgery Association (IVSA)
2015-12-01
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| Series: | Iranian Journal of Veterinary Surgery |
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| Online Access: | http://www.ivsajournals.com/article_12275_11e4e0d14c5bf7b2f3741723c4384492.pdf |
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| author | Behzad Pourreza Seyed Mehdi Ghamsari Farhang Sasani frajollah Adib hashemi Hamed Mansoor Lakooraj |
| author_facet | Behzad Pourreza Seyed Mehdi Ghamsari Farhang Sasani frajollah Adib hashemi Hamed Mansoor Lakooraj |
| author_sort | Behzad Pourreza |
| collection | DOAJ |
| description | <br /> Objective- Mechanisms that decrease oxidative stress and enhance peritoneal fibrinolysis reduce adhesions. N-acetyl-L-cysteine (NAC) is an antioxidant whose effect on peritoneal fibrinolysis in large animal model has not been established. The aims of this study were to investigate the ability of NAC to decreased adhesion in stablished model of serosal trauma in sheep.<br /> Design- Experimental study<br /> Animals- Eight healthy male sheep weighting 42.67 ± 2.31 kg were used in this study.<br /> Procedures- Established model of serosal trauma were used for adhesion induction. Each sheep intreated group (n=4) received NAC (150 mg/kg) intraperitonealy on operative day and Intravenous infusion during 7days after surgery. Animals in untreated group received normal saline instead of NAC. Blood samples for evaluation of the CBC, total protein and fibrinogen were obtained on perioperative day and at days 1, 2, 4, 7, 11 and 14 after surgery. Animals were killed 14 days postoperatively and Adhesion formation was scored macroscopically and histopathologically.<br /> Results- Evaluation of CBC showed inflammation in 75% of animals in untreated group. CBC of animals in treated group was normal during the study. Measuring the fibrinogen concentration revealed significant differences between untreated and treated groups. Extensive of adhesion formation was 100% in all sheep in both 2 groups. Macroscopic evaluation of severity of adhesion and histopathological assessment of inflammation and fibrosis showed significant reduction in adhesion formation in treated animals.<br /> Conclusions and Clinical Relevance- Results of our study suggest a potential therapeutic use for N-Acetylcysteine in adhesion reduction and prevention in human and veterinary medicine. |
| first_indexed | 2024-12-23T19:50:26Z |
| format | Article |
| id | doaj.art-9bbd2745bca84f5cace34096289a46e3 |
| institution | Directory Open Access Journal |
| issn | 2008-3033 2008-3033 |
| language | English |
| last_indexed | 2024-12-23T19:50:26Z |
| publishDate | 2015-12-01 |
| publisher | Iranian Veterinary Surgery Association (IVSA) |
| record_format | Article |
| series | Iranian Journal of Veterinary Surgery |
| spelling | doaj.art-9bbd2745bca84f5cace34096289a46e32022-12-21T17:33:24ZengIranian Veterinary Surgery Association (IVSA)Iranian Journal of Veterinary Surgery2008-30332008-30332015-12-0110211012275Effects of N-acetyl-L-cysteine on Postoperative Intrabdominal Adhesion in a Large Animal ModelBehzad Pourreza0Seyed Mehdi Ghamsari1Farhang Sasani2frajollah Adib hashemi3Hamed Mansoor Lakooraj4Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran- IranDepartment of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran- IranDepartment of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Tehran-IranDepartment of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran- IranDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran<br /> Objective- Mechanisms that decrease oxidative stress and enhance peritoneal fibrinolysis reduce adhesions. N-acetyl-L-cysteine (NAC) is an antioxidant whose effect on peritoneal fibrinolysis in large animal model has not been established. The aims of this study were to investigate the ability of NAC to decreased adhesion in stablished model of serosal trauma in sheep.<br /> Design- Experimental study<br /> Animals- Eight healthy male sheep weighting 42.67 ± 2.31 kg were used in this study.<br /> Procedures- Established model of serosal trauma were used for adhesion induction. Each sheep intreated group (n=4) received NAC (150 mg/kg) intraperitonealy on operative day and Intravenous infusion during 7days after surgery. Animals in untreated group received normal saline instead of NAC. Blood samples for evaluation of the CBC, total protein and fibrinogen were obtained on perioperative day and at days 1, 2, 4, 7, 11 and 14 after surgery. Animals were killed 14 days postoperatively and Adhesion formation was scored macroscopically and histopathologically.<br /> Results- Evaluation of CBC showed inflammation in 75% of animals in untreated group. CBC of animals in treated group was normal during the study. Measuring the fibrinogen concentration revealed significant differences between untreated and treated groups. Extensive of adhesion formation was 100% in all sheep in both 2 groups. Macroscopic evaluation of severity of adhesion and histopathological assessment of inflammation and fibrosis showed significant reduction in adhesion formation in treated animals.<br /> Conclusions and Clinical Relevance- Results of our study suggest a potential therapeutic use for N-Acetylcysteine in adhesion reduction and prevention in human and veterinary medicine.http://www.ivsajournals.com/article_12275_11e4e0d14c5bf7b2f3741723c4384492.pdfNAC (N-Acetylcysteine)intraabdominal adhesion |
| spellingShingle | Behzad Pourreza Seyed Mehdi Ghamsari Farhang Sasani frajollah Adib hashemi Hamed Mansoor Lakooraj Effects of N-acetyl-L-cysteine on Postoperative Intrabdominal Adhesion in a Large Animal Model Iranian Journal of Veterinary Surgery NAC (N-Acetylcysteine) intraabdominal adhesion |
| title | Effects of N-acetyl-L-cysteine on Postoperative Intrabdominal Adhesion in a Large Animal Model |
| title_full | Effects of N-acetyl-L-cysteine on Postoperative Intrabdominal Adhesion in a Large Animal Model |
| title_fullStr | Effects of N-acetyl-L-cysteine on Postoperative Intrabdominal Adhesion in a Large Animal Model |
| title_full_unstemmed | Effects of N-acetyl-L-cysteine on Postoperative Intrabdominal Adhesion in a Large Animal Model |
| title_short | Effects of N-acetyl-L-cysteine on Postoperative Intrabdominal Adhesion in a Large Animal Model |
| title_sort | effects of n acetyl l cysteine on postoperative intrabdominal adhesion in a large animal model |
| topic | NAC (N-Acetylcysteine) intraabdominal adhesion |
| url | http://www.ivsajournals.com/article_12275_11e4e0d14c5bf7b2f3741723c4384492.pdf |
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