Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects
Initially discovered over 35 years ago in the bacterium <i>Escherichia coli</i> as a defense system against invasion of viral (or other exogenous) DNA into the genome, CRISPR/Cas has ushered in a new era of functional genetics and served as a versatile genetic tool in all branches of lif...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/12/1/118 |
| _version_ | 1797339627319721984 |
|---|---|
| author | Jiashun Li Shuaishuai Wu Kaidian Zhang Xueqiong Sun Wenwen Lin Cong Wang Senjie Lin |
| author_facet | Jiashun Li Shuaishuai Wu Kaidian Zhang Xueqiong Sun Wenwen Lin Cong Wang Senjie Lin |
| author_sort | Jiashun Li |
| collection | DOAJ |
| description | Initially discovered over 35 years ago in the bacterium <i>Escherichia coli</i> as a defense system against invasion of viral (or other exogenous) DNA into the genome, CRISPR/Cas has ushered in a new era of functional genetics and served as a versatile genetic tool in all branches of life science. CRISPR/Cas has revolutionized the methodology of gene knockout with simplicity and rapidity, but it is also powerful for gene knock-in and gene modification. In the field of marine biology and ecology, this tool has been instrumental in the functional characterization of ‘dark’ genes and the documentation of the functional differentiation of gene paralogs. Powerful as it is, challenges exist that have hindered the advances in functional genetics in some important lineages. This review examines the status of applications of CRISPR/Cas in marine research and assesses the prospect of quickly expanding the deployment of this powerful tool to address the myriad fundamental marine biology and biological oceanography questions. |
| first_indexed | 2024-03-08T09:49:58Z |
| format | Article |
| id | doaj.art-9bc0249b53c643768069a4fe126dba9d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T09:49:58Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9bc0249b53c643768069a4fe126dba9d2024-01-29T14:06:24ZengMDPI AGMicroorganisms2076-26072024-01-0112111810.3390/microorganisms12010118Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and ProspectsJiashun Li0Shuaishuai Wu1Kaidian Zhang2Xueqiong Sun3Wenwen Lin4Cong Wang5Senjie Lin6State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361101, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361101, ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, School of Marine Biology and Fisheries, Hainan University, Haikou 570203, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361101, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361101, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361101, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361101, ChinaInitially discovered over 35 years ago in the bacterium <i>Escherichia coli</i> as a defense system against invasion of viral (or other exogenous) DNA into the genome, CRISPR/Cas has ushered in a new era of functional genetics and served as a versatile genetic tool in all branches of life science. CRISPR/Cas has revolutionized the methodology of gene knockout with simplicity and rapidity, but it is also powerful for gene knock-in and gene modification. In the field of marine biology and ecology, this tool has been instrumental in the functional characterization of ‘dark’ genes and the documentation of the functional differentiation of gene paralogs. Powerful as it is, challenges exist that have hindered the advances in functional genetics in some important lineages. This review examines the status of applications of CRISPR/Cas in marine research and assesses the prospect of quickly expanding the deployment of this powerful tool to address the myriad fundamental marine biology and biological oceanography questions.https://www.mdpi.com/2076-2607/12/1/118CRISPR/Casgenome editing technologymarine biologyfunctional genetic researchmarine prokaryotic microbesphytoplankton |
| spellingShingle | Jiashun Li Shuaishuai Wu Kaidian Zhang Xueqiong Sun Wenwen Lin Cong Wang Senjie Lin Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects Microorganisms CRISPR/Cas genome editing technology marine biology functional genetic research marine prokaryotic microbes phytoplankton |
| title | Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects |
| title_full | Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects |
| title_fullStr | Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects |
| title_full_unstemmed | Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects |
| title_short | Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects |
| title_sort | clustered regularly interspaced short palindromic repeat crispr associated protein and its utility all at sea status challenges and prospects |
| topic | CRISPR/Cas genome editing technology marine biology functional genetic research marine prokaryotic microbes phytoplankton |
| url | https://www.mdpi.com/2076-2607/12/1/118 |
| work_keys_str_mv | AT jiashunli clusteredregularlyinterspacedshortpalindromicrepeatcrisprassociatedproteinanditsutilityallatseastatuschallengesandprospects AT shuaishuaiwu clusteredregularlyinterspacedshortpalindromicrepeatcrisprassociatedproteinanditsutilityallatseastatuschallengesandprospects AT kaidianzhang clusteredregularlyinterspacedshortpalindromicrepeatcrisprassociatedproteinanditsutilityallatseastatuschallengesandprospects AT xueqiongsun clusteredregularlyinterspacedshortpalindromicrepeatcrisprassociatedproteinanditsutilityallatseastatuschallengesandprospects AT wenwenlin clusteredregularlyinterspacedshortpalindromicrepeatcrisprassociatedproteinanditsutilityallatseastatuschallengesandprospects AT congwang clusteredregularlyinterspacedshortpalindromicrepeatcrisprassociatedproteinanditsutilityallatseastatuschallengesandprospects AT senjielin clusteredregularlyinterspacedshortpalindromicrepeatcrisprassociatedproteinanditsutilityallatseastatuschallengesandprospects |