Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules
ABSTRACTLegumes can contribute to emissions of the potent greenhouse gas nitrous oxide (N2O) directly by some rhizobia species that are able to denitrify under free-living conditions and in symbiotic association with the plant. In this study, the capacity of Phaseolus vulgaris-Rhizobium etli symbios...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Plant Interactions |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/17429145.2023.2251511 |
| _version_ | 1797245135941009408 |
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| author | Alba Hidalgo-García Germán Tortosa Pedro J. Pacheco Andrew J. Gates David J. Richardson Eulogio J. Bedmar Lourdes Girard María J. Torres María J. Delgado |
| author_facet | Alba Hidalgo-García Germán Tortosa Pedro J. Pacheco Andrew J. Gates David J. Richardson Eulogio J. Bedmar Lourdes Girard María J. Torres María J. Delgado |
| author_sort | Alba Hidalgo-García |
| collection | DOAJ |
| description | ABSTRACTLegumes can contribute to emissions of the potent greenhouse gas nitrous oxide (N2O) directly by some rhizobia species that are able to denitrify under free-living conditions and in symbiotic association with the plant. In this study, the capacity of Phaseolus vulgaris-Rhizobium etli symbiosis to emit N2O in response to nitrate (NO3-) has been demonstrated for the first time. We found that bacteroidal assimilatory nitrate reductase (NarB) as well as nitrite reductase (NirK) and nitric oxide reductase (cNor) denitrifying enzymes contribute to nitric oxide (NO) and N2O formation in nodules. We also show that R. etli NarK is involved in NO2- extrusion and links NO3- reduction by NarB in the cytoplasm with NirK and cNor denitrification activities in the periplasm. The knowledge generated in this work will be instrumental for exploring strategies and sustainable practices in agricultural soil management to increase legume crop yield and mitigate greenhouse gas emissions. |
| first_indexed | 2024-03-11T10:27:50Z |
| format | Article |
| id | doaj.art-9bc2d5205f504f4cab5efa3ba65366ee |
| institution | Directory Open Access Journal |
| issn | 1742-9145 1742-9153 |
| language | English |
| last_indexed | 2024-04-24T19:22:06Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj.art-9bc2d5205f504f4cab5efa3ba65366ee2024-03-25T17:45:03ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532023-12-0118110.1080/17429145.2023.2251511Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodulesAlba Hidalgo-García0Germán Tortosa1Pedro J. Pacheco2Andrew J. Gates3David J. Richardson4Eulogio J. Bedmar5Lourdes Girard6María J. Torres7María J. Delgado8Department of Soil and Plant Microbiology, Estación Experimental del Zaidín, CSIC, Granada, SpainDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín, CSIC, Granada, SpainDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín, CSIC, Granada, SpainSchool of Biological Sciences, University of East Anglia, Norwich, UKSchool of Biological Sciences, University of East Anglia, Norwich, UKDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín, CSIC, Granada, SpainGenomic Microbiology Program, Centro de Ciencias Genómicas, National Autonomous University of Mexico, Morelos, MéxicoDepartment of Biochemistry and Molecular Biology, Campus Universitario de Rabanales, University of Córdoba, Córdoba, SpainDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín, CSIC, Granada, SpainABSTRACTLegumes can contribute to emissions of the potent greenhouse gas nitrous oxide (N2O) directly by some rhizobia species that are able to denitrify under free-living conditions and in symbiotic association with the plant. In this study, the capacity of Phaseolus vulgaris-Rhizobium etli symbiosis to emit N2O in response to nitrate (NO3-) has been demonstrated for the first time. We found that bacteroidal assimilatory nitrate reductase (NarB) as well as nitrite reductase (NirK) and nitric oxide reductase (cNor) denitrifying enzymes contribute to nitric oxide (NO) and N2O formation in nodules. We also show that R. etli NarK is involved in NO2- extrusion and links NO3- reduction by NarB in the cytoplasm with NirK and cNor denitrification activities in the periplasm. The knowledge generated in this work will be instrumental for exploring strategies and sustainable practices in agricultural soil management to increase legume crop yield and mitigate greenhouse gas emissions.https://www.tandfonline.com/doi/10.1080/17429145.2023.2251511Bacteroidsdenitrificationnitrate reductasenitric oxide reductasenitrite reductasenitrite transporter |
| spellingShingle | Alba Hidalgo-García Germán Tortosa Pedro J. Pacheco Andrew J. Gates David J. Richardson Eulogio J. Bedmar Lourdes Girard María J. Torres María J. Delgado Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules Journal of Plant Interactions Bacteroids denitrification nitrate reductase nitric oxide reductase nitrite reductase nitrite transporter |
| title | Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules |
| title_full | Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules |
| title_fullStr | Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules |
| title_full_unstemmed | Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules |
| title_short | Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules |
| title_sort | rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules |
| topic | Bacteroids denitrification nitrate reductase nitric oxide reductase nitrite reductase nitrite transporter |
| url | https://www.tandfonline.com/doi/10.1080/17429145.2023.2251511 |
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