Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory
Background: This study aimed to create an in-house glucose quality control material for humans, addressing the challenge of obtaining high-cost commercially prepared quality control materials in developing countries. Methods: An in-house quality control material for glucose was prepared using a pool...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Practical Laboratory Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551724000234 |
| _version_ | 1797262867677839360 |
|---|---|
| author | Haymanot Tewabe Asaye Mitiku Abebe Yenesew |
| author_facet | Haymanot Tewabe Asaye Mitiku Abebe Yenesew |
| author_sort | Haymanot Tewabe |
| collection | DOAJ |
| description | Background: This study aimed to create an in-house glucose quality control material for humans, addressing the challenge of obtaining high-cost commercially prepared quality control materials in developing countries. Methods: An in-house quality control material for glucose was prepared using a pooled serum sample and analyzed using a fully automated chemistry analyzer following the ISO 80 guidelines. The mean, standard deviation (SD), and coefficient of variance were calculated from the first 30 days of measurement, and the variability was checked over eight months using SPSS software. The study used Pearson's correlation with a 95% confidence interval and a P-value less than 0.05, which was statistically significant. Results: The average mean ± SD of human serum glucose was 185.2 ± 8.4 mg/dL, indicating that the precision between each measurement was better. The prepared in-house quality control material was stable for approximately five months without any significant change in the serum glucose concentration (mg/dl) (p-value<0.05). Conclusions: The study suggests that room-temperature, 2–8 °C, and −20 °C to −30 °C storage of human serum samples for glucose analysis is a viable option, with stable glucose concentrations for up to 30 days. Pooled serum is a cost-effective method for in-house quality control, especially in resource-limited laboratories. |
| first_indexed | 2024-04-25T00:03:56Z |
| format | Article |
| id | doaj.art-9bc59e8c476f479a967b7279906e881a |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-04-25T00:03:56Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-9bc59e8c476f479a967b7279906e881a2024-03-14T06:15:08ZengElsevierPractical Laboratory Medicine2352-55172024-03-0139e00377Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratoryHaymanot Tewabe0Asaye Mitiku1Abebe Yenesew2Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Debre Markos University, Debre Markos, Ethiopia; Corresponding author: P.O.Box 269, Ethiopia.Department of Medical Laboratory Sciences, College of Health Sciences, Dilla University, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Debre Markos University, Debre Markos, EthiopiaBackground: This study aimed to create an in-house glucose quality control material for humans, addressing the challenge of obtaining high-cost commercially prepared quality control materials in developing countries. Methods: An in-house quality control material for glucose was prepared using a pooled serum sample and analyzed using a fully automated chemistry analyzer following the ISO 80 guidelines. The mean, standard deviation (SD), and coefficient of variance were calculated from the first 30 days of measurement, and the variability was checked over eight months using SPSS software. The study used Pearson's correlation with a 95% confidence interval and a P-value less than 0.05, which was statistically significant. Results: The average mean ± SD of human serum glucose was 185.2 ± 8.4 mg/dL, indicating that the precision between each measurement was better. The prepared in-house quality control material was stable for approximately five months without any significant change in the serum glucose concentration (mg/dl) (p-value<0.05). Conclusions: The study suggests that room-temperature, 2–8 °C, and −20 °C to −30 °C storage of human serum samples for glucose analysis is a viable option, with stable glucose concentrations for up to 30 days. Pooled serum is a cost-effective method for in-house quality control, especially in resource-limited laboratories.http://www.sciencedirect.com/science/article/pii/S2352551724000234Inhouse quality controlGlucoseValidation |
| spellingShingle | Haymanot Tewabe Asaye Mitiku Abebe Yenesew Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory Practical Laboratory Medicine Inhouse quality control Glucose Validation |
| title | Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory |
| title_full | Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory |
| title_fullStr | Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory |
| title_full_unstemmed | Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory |
| title_short | Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory |
| title_sort | validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory |
| topic | Inhouse quality control Glucose Validation |
| url | http://www.sciencedirect.com/science/article/pii/S2352551724000234 |
| work_keys_str_mv | AT haymanottewabe validationoftheefficacyofpooledserumforserumglucoseinhousequalitycontrolmaterialincomparisonwithcommercialinternalqualitycontrolinclinicalchemistrylaboratory AT asayemitiku validationoftheefficacyofpooledserumforserumglucoseinhousequalitycontrolmaterialincomparisonwithcommercialinternalqualitycontrolinclinicalchemistrylaboratory AT abebeyenesew validationoftheefficacyofpooledserumforserumglucoseinhousequalitycontrolmaterialincomparisonwithcommercialinternalqualitycontrolinclinicalchemistrylaboratory |