Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4.
The emergence of novel pathogens poses a major public health threat causing widespread epidemics in susceptible populations. The Escherichia coli O104:H4 strain implicated in a 2011 outbreak in northern Germany caused the highest frequency of hemolytic uremic syndrome (HUS) and death ever recorded i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3405000?pdf=render |
| _version_ | 1828937637028691968 |
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| author | Rim Al Safadi Galeb S Abu-Ali Rudolph E Sloup James T Rudrik Christopher M Waters Kathryn A Eaton Shannon D Manning |
| author_facet | Rim Al Safadi Galeb S Abu-Ali Rudolph E Sloup James T Rudrik Christopher M Waters Kathryn A Eaton Shannon D Manning |
| author_sort | Rim Al Safadi |
| collection | DOAJ |
| description | The emergence of novel pathogens poses a major public health threat causing widespread epidemics in susceptible populations. The Escherichia coli O104:H4 strain implicated in a 2011 outbreak in northern Germany caused the highest frequency of hemolytic uremic syndrome (HUS) and death ever recorded in a single E. coli outbreak. Therefore, it has been suggested that this strain is more virulent than other pathogenic E. coli (e.g., E. coli O157:H7). The E. coli O104:H4 outbreak strain possesses multiple virulence factors from both Shiga toxin (Stx)-producing E. coli (STEC) and enteroaggregative E. coli (EAEC), though the mechanism of pathogenesis is not known. Here, we demonstrate that E. coli O104:H4 produces a stable biofilm in vitro and that in vivo virulence gene expression is highest when E. coli O104:H4 overexpresses genes required for aggregation and exopolysaccharide production, a characteristic of bacterial cells residing within an established biofilm. Interrupting exopolysaccharide production and biofilm formation may therefore represent effective strategies for combating future E. coli O104:H4 infections. |
| first_indexed | 2024-12-14T02:27:14Z |
| format | Article |
| id | doaj.art-9bc716235de2454ea5395bc34280d525 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T02:27:14Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9bc716235de2454ea5395bc34280d5252022-12-21T23:20:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4162810.1371/journal.pone.0041628Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4.Rim Al SafadiGaleb S Abu-AliRudolph E SloupJames T RudrikChristopher M WatersKathryn A EatonShannon D ManningThe emergence of novel pathogens poses a major public health threat causing widespread epidemics in susceptible populations. The Escherichia coli O104:H4 strain implicated in a 2011 outbreak in northern Germany caused the highest frequency of hemolytic uremic syndrome (HUS) and death ever recorded in a single E. coli outbreak. Therefore, it has been suggested that this strain is more virulent than other pathogenic E. coli (e.g., E. coli O157:H7). The E. coli O104:H4 outbreak strain possesses multiple virulence factors from both Shiga toxin (Stx)-producing E. coli (STEC) and enteroaggregative E. coli (EAEC), though the mechanism of pathogenesis is not known. Here, we demonstrate that E. coli O104:H4 produces a stable biofilm in vitro and that in vivo virulence gene expression is highest when E. coli O104:H4 overexpresses genes required for aggregation and exopolysaccharide production, a characteristic of bacterial cells residing within an established biofilm. Interrupting exopolysaccharide production and biofilm formation may therefore represent effective strategies for combating future E. coli O104:H4 infections.http://europepmc.org/articles/PMC3405000?pdf=render |
| spellingShingle | Rim Al Safadi Galeb S Abu-Ali Rudolph E Sloup James T Rudrik Christopher M Waters Kathryn A Eaton Shannon D Manning Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4. PLoS ONE |
| title | Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4. |
| title_full | Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4. |
| title_fullStr | Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4. |
| title_full_unstemmed | Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4. |
| title_short | Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4. |
| title_sort | correlation between in vivo biofilm formation and virulence gene expression in escherichia coli o104 h4 |
| url | http://europepmc.org/articles/PMC3405000?pdf=render |
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