Insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with SO42− and heavy metals
The increased demand for rare earth resources has led to an increase in the development of rare earth mines (REMs). However, the production of high-concentration leaching agents (SO42−) and heavy metals as a result of rare earth mining has increased, necessitating the removal of contaminants. Here,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1050635/full |
| _version_ | 1797862047668502528 |
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| author | Xiao Yan Xiao Yan Bowen Gao Bowen Gao Jianlei Wang Jianlei Wang Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Mingjiang Zhang Mingjiang Zhang |
| author_facet | Xiao Yan Xiao Yan Bowen Gao Bowen Gao Jianlei Wang Jianlei Wang Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Mingjiang Zhang Mingjiang Zhang |
| author_sort | Xiao Yan |
| collection | DOAJ |
| description | The increased demand for rare earth resources has led to an increase in the development of rare earth mines (REMs). However, the production of high-concentration leaching agents (SO42−) and heavy metals as a result of rare earth mining has increased, necessitating the removal of contaminants. Here, a series of experiments with different remediation measures, including control (CK), sulfate-reducing bacteria (SRB) alone (M), chemicals (Ca(OH)2, 1.5 g/kg) plus SRB (CM-L), chemicals (Ca(OH)2, 3.0 g/kg) plus SRB (CM-M), and chemicals (Ca(OH)2, 4.5 g/kg) plus SRB (CM-H), were conducted to investigate the removal effect of SO42−, Pb, Zn, and Mn from the REM soil. Then, a high-throughput sequencing technology was applied to explore the response of bacterial community diversity and functions with different remediation measures. The results indicated that CM-M treatment had a more efficient removal effect for SO42−, Pb, Zn, and Mn than the others, up to 94.6, 88.3, 98.7, and 91%, respectively. Soil bacterial abundance and diversity were significantly affected by treatments with the inoculation of SRB in comparison with CK. The relative abundance of Desulfobacterota with the ability to transform SO42− into S2− increased significantly in all treatments, except for CK. There was a strong correlation between environmental factors (pH, Eh, SO42−, Pb, and Zn) and bacterial community structure. Furthermore, functional prediction analysis revealed that the SRB inoculation treatments significantly increased the abundance of sulfate respiration, sulfite respiration, and nitrogen fixation, while decreasing the abundance of manganese oxidation, dark hydrogen oxidation, and denitrification. This provides good evidence for us to understand the difference in removal efficiency, bacterial community structure, and function by different remediation measures that help select a more efficient and sustainable method to remediate contaminants in the REM soil. |
| first_indexed | 2024-04-09T22:13:00Z |
| format | Article |
| id | doaj.art-9bc7c8309bb74bb6bfa30a5c1172b611 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T22:13:00Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-9bc7c8309bb74bb6bfa30a5c1172b6112023-03-23T06:43:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.10506351050635Insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with SO42− and heavy metalsXiao Yan0Xiao Yan1Bowen Gao2Bowen Gao3Jianlei Wang4Jianlei Wang5Xuezhe Zhu6Xuezhe Zhu7Xuezhe Zhu8Xuezhe Zhu9Mingjiang Zhang10Mingjiang Zhang11GRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-Friendly Metallurgy in Producing Premium Non-Ferrous Metals, GRINM Group Co., Ltd., Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-Friendly Metallurgy in Producing Premium Non-Ferrous Metals, GRINM Group Co., Ltd., Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-Friendly Metallurgy in Producing Premium Non-Ferrous Metals, GRINM Group Co., Ltd., Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-Friendly Metallurgy in Producing Premium Non-Ferrous Metals, GRINM Group Co., Ltd., Beijing, ChinaSchool of Metallurgy, Northeastern University, Shenyang, ChinaGRIMAT Engineering Institute Co., Ltd., Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-Friendly Metallurgy in Producing Premium Non-Ferrous Metals, GRINM Group Co., Ltd., Beijing, ChinaThe increased demand for rare earth resources has led to an increase in the development of rare earth mines (REMs). However, the production of high-concentration leaching agents (SO42−) and heavy metals as a result of rare earth mining has increased, necessitating the removal of contaminants. Here, a series of experiments with different remediation measures, including control (CK), sulfate-reducing bacteria (SRB) alone (M), chemicals (Ca(OH)2, 1.5 g/kg) plus SRB (CM-L), chemicals (Ca(OH)2, 3.0 g/kg) plus SRB (CM-M), and chemicals (Ca(OH)2, 4.5 g/kg) plus SRB (CM-H), were conducted to investigate the removal effect of SO42−, Pb, Zn, and Mn from the REM soil. Then, a high-throughput sequencing technology was applied to explore the response of bacterial community diversity and functions with different remediation measures. The results indicated that CM-M treatment had a more efficient removal effect for SO42−, Pb, Zn, and Mn than the others, up to 94.6, 88.3, 98.7, and 91%, respectively. Soil bacterial abundance and diversity were significantly affected by treatments with the inoculation of SRB in comparison with CK. The relative abundance of Desulfobacterota with the ability to transform SO42− into S2− increased significantly in all treatments, except for CK. There was a strong correlation between environmental factors (pH, Eh, SO42−, Pb, and Zn) and bacterial community structure. Furthermore, functional prediction analysis revealed that the SRB inoculation treatments significantly increased the abundance of sulfate respiration, sulfite respiration, and nitrogen fixation, while decreasing the abundance of manganese oxidation, dark hydrogen oxidation, and denitrification. This provides good evidence for us to understand the difference in removal efficiency, bacterial community structure, and function by different remediation measures that help select a more efficient and sustainable method to remediate contaminants in the REM soil.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1050635/fullsulfate-reducing bacteria (SRB)SO42−heavy metalsrare earth mines soilsoil bacterial communitybacterial function |
| spellingShingle | Xiao Yan Xiao Yan Bowen Gao Bowen Gao Jianlei Wang Jianlei Wang Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Mingjiang Zhang Mingjiang Zhang Insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with SO42− and heavy metals Frontiers in Microbiology sulfate-reducing bacteria (SRB) SO42− heavy metals rare earth mines soil soil bacterial community bacterial function |
| title | Insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with SO42− and heavy metals |
| title_full | Insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with SO42− and heavy metals |
| title_fullStr | Insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with SO42− and heavy metals |
| title_full_unstemmed | Insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with SO42− and heavy metals |
| title_short | Insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with SO42− and heavy metals |
| title_sort | insights into remediation effects and bacterial diversity of different remediation measures in rare earth mine soil with so42 and heavy metals |
| topic | sulfate-reducing bacteria (SRB) SO42− heavy metals rare earth mines soil soil bacterial community bacterial function |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1050635/full |
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