The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit <i>Clostridium perfringens</i> Growth
<i>Clostridium perfringens</i> is an important pathogen for both humans and animals, causing human foodborne disease and necrotic enteritis in poultry. In the present study, a <i>C. perfringens</i>-specific phage, vB_CpeS_BG3P (designated as BG3P hereafter), was isolated from...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/4/676 |
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| author | Sisi Huang Yuan Tian Yongjuan Wang Pilar García Banhong Liu Rui Lu Liting Wu Hongduo Bao Maoda Pang Yan Zhou Ran Wang Hui Zhang |
| author_facet | Sisi Huang Yuan Tian Yongjuan Wang Pilar García Banhong Liu Rui Lu Liting Wu Hongduo Bao Maoda Pang Yan Zhou Ran Wang Hui Zhang |
| author_sort | Sisi Huang |
| collection | DOAJ |
| description | <i>Clostridium perfringens</i> is an important pathogen for both humans and animals, causing human foodborne disease and necrotic enteritis in poultry. In the present study, a <i>C. perfringens</i>-specific phage, vB_CpeS_BG3P (designated as BG3P hereafter), was isolated from chicken farm sewage. Both electron microscopy and phylogenetic analysis suggested that phage BG3P is a novel phage belonging to <i>Siphoviridae</i> family. Phage BG3P exhibited a broad host range against different <i>C. perfringens</i> isolates (90.63% of strains were infected). Sequencing of the complete genome revealed a linear double-stranded DNA (43,528 bp) with 28.65% GC content. After sequence analysis, 73 open reading frames (<i>orf</i>s) were predicted, of which only 13 were annotated with known functions. No tRNA and virulence encoding genes were detected. It should be noted that the protein of <i>orf 15</i> has 97.92% homology to <i>C. perfringens</i>-specific chloramphenicol resistance protein, which has not been reported for any <i>C. perfringens</i> phage. Phylogenetic analysis of the ssDNA binding protein demonstrated that this phage is closely related to <i>C. perfringens</i> phages phiSM101 and phi3626. In considering future use as an antimicrobial agent, some biological characteristics were observed, such as a good pH (3–11) stability and moderate temperature tolerance (<60 °C). Moreover, bacteriophage BG3P showed a good antimicrobial effect against <i>C. perfringens</i> liquid cultures. Thus, phage treatment with MOI ≥ 100 completely inhibited bacterial growth compared to untreated cultures. Although phage BG3P shows good lytic efficiency and broad host range in vitro, future development and application may need to consider removal of the chloramphenicol-like resistance gene or exploring its lysin for future antibacterial applications. |
| first_indexed | 2024-03-09T12:53:05Z |
| format | Article |
| id | doaj.art-9bc9692c9f8247eaad1eed20fa48deb5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:53:05Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-9bc9692c9f8247eaad1eed20fa48deb52023-11-30T22:03:09ZengMDPI AGViruses1999-49152022-03-0114467610.3390/v14040676The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit <i>Clostridium perfringens</i> GrowthSisi Huang0Yuan Tian1Yongjuan Wang2Pilar García3Banhong Liu4Rui Lu5Liting Wu6Hongduo Bao7Maoda Pang8Yan Zhou9Ran Wang10Hui Zhang11College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, ChinaDairy Research Institute of Asturias (IPLA-CSIC), 33300 Villaviciosa, Asturias, SpainJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China<i>Clostridium perfringens</i> is an important pathogen for both humans and animals, causing human foodborne disease and necrotic enteritis in poultry. In the present study, a <i>C. perfringens</i>-specific phage, vB_CpeS_BG3P (designated as BG3P hereafter), was isolated from chicken farm sewage. Both electron microscopy and phylogenetic analysis suggested that phage BG3P is a novel phage belonging to <i>Siphoviridae</i> family. Phage BG3P exhibited a broad host range against different <i>C. perfringens</i> isolates (90.63% of strains were infected). Sequencing of the complete genome revealed a linear double-stranded DNA (43,528 bp) with 28.65% GC content. After sequence analysis, 73 open reading frames (<i>orf</i>s) were predicted, of which only 13 were annotated with known functions. No tRNA and virulence encoding genes were detected. It should be noted that the protein of <i>orf 15</i> has 97.92% homology to <i>C. perfringens</i>-specific chloramphenicol resistance protein, which has not been reported for any <i>C. perfringens</i> phage. Phylogenetic analysis of the ssDNA binding protein demonstrated that this phage is closely related to <i>C. perfringens</i> phages phiSM101 and phi3626. In considering future use as an antimicrobial agent, some biological characteristics were observed, such as a good pH (3–11) stability and moderate temperature tolerance (<60 °C). Moreover, bacteriophage BG3P showed a good antimicrobial effect against <i>C. perfringens</i> liquid cultures. Thus, phage treatment with MOI ≥ 100 completely inhibited bacterial growth compared to untreated cultures. Although phage BG3P shows good lytic efficiency and broad host range in vitro, future development and application may need to consider removal of the chloramphenicol-like resistance gene or exploring its lysin for future antibacterial applications.https://www.mdpi.com/1999-4915/14/4/676<i>Clostridium perfringens</i>phageantimicrobial agentpoultry industry |
| spellingShingle | Sisi Huang Yuan Tian Yongjuan Wang Pilar García Banhong Liu Rui Lu Liting Wu Hongduo Bao Maoda Pang Yan Zhou Ran Wang Hui Zhang The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit <i>Clostridium perfringens</i> Growth Viruses <i>Clostridium perfringens</i> phage antimicrobial agent poultry industry |
| title | The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit <i>Clostridium perfringens</i> Growth |
| title_full | The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit <i>Clostridium perfringens</i> Growth |
| title_fullStr | The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit <i>Clostridium perfringens</i> Growth |
| title_full_unstemmed | The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit <i>Clostridium perfringens</i> Growth |
| title_short | The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit <i>Clostridium perfringens</i> Growth |
| title_sort | broad host range phage vb cpes bg3p is able to inhibit i clostridium perfringens i growth |
| topic | <i>Clostridium perfringens</i> phage antimicrobial agent poultry industry |
| url | https://www.mdpi.com/1999-4915/14/4/676 |
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