IN SILICO AND IN VITRO EVALUATION OF ENROFLOXACIN ON AFLATOXIN B1-INDUCED CYTOTOXICITY
Additional therapeutics are required to minimize the toxicity of aflatoxin B1 (AFB1) in animals due to the drawbacks of mycotoxin binders. The cytochrome P450 enzyme system is necessary for the metabolic activation of AFB1 before it produces the cytotoxic AFB1-exo-8, 9-epoxide (AFBO). It is alrea...
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| Format: | Article |
| Language: | English |
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West Bengal Veterinary Alumni Association
2023-12-01
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| Series: | Exploratory Animal and Medical Research |
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| Online Access: | https://animalmedicalresearch.org/Vol.13_Issue-2_December_2023/In%20silico%20and%20in%20vitro%20evaluationof%20enrofloxacin%20on%20aflatoxin%20B1-induced%20cytotoxicity.pdf |
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| author | T. Jagadeesh M. Parthiban P. Raja Ghadevaru Sarathchandra S. Vairamuthu |
| author_facet | T. Jagadeesh M. Parthiban P. Raja Ghadevaru Sarathchandra S. Vairamuthu |
| author_sort | T. Jagadeesh |
| collection | DOAJ |
| description | Additional therapeutics are required to minimize the toxicity of aflatoxin B1 (AFB1) in
animals due to the drawbacks of mycotoxin binders. The cytochrome P450 enzyme system is necessary for
the metabolic activation of AFB1 before it produces the cytotoxic AFB1-exo-8, 9-epoxide (AFBO). It is
already proven that enrofloxacin (ENR) has been shown to substantially decrease the activity of the
cytochrome P450 enzymes. To understand how ENR affects AFB1-induced cytotoxicity, it is important to
highlight this context. In this present study, molecular docking was performed between ENR with
CYP3A4 protein, apoptotic proteins (Bax, caspase 3, caspase 8, fas L, MAPK1), and catalase. To assess
the effect of ENR on AFB1-induced cytotoxicity by MTT assay and trypan blue dye exclusion techniques,
the mammalian simulative Vero cell lines were used in different treatment groups as AFB1 alone, ENR
alone, AFB1+ENR, and AFB1+silymarin (known cell protective agent). This was done to further evaluate
the in silico analysis. ENR interacted more positively with all of the proteins (CYP3A4, Bax, catalase,
caspase 3, caspase 8, MAPK1, and fas L), according to the findings of molecular docking studies. On the
other hand, cytotoxicity caused by AFB1 is successfully prevented by ENR at a dose of 25 µg/mL. The
results of the present study suggest that ENR primarily protects against cytotoxicity induced by AFB1 in
Vero cells as the evidence of in silico studies revealed that ENR may protect AFB1-induced cytotoxicity by
interacting with the CYP3A4 enzyme which is primarily required for activation AFB1. |
| first_indexed | 2024-03-08T23:31:58Z |
| format | Article |
| id | doaj.art-9bc99354dd25413b9f8126b81a771bb1 |
| institution | Directory Open Access Journal |
| issn | 2277-470X 2319-247X |
| language | English |
| last_indexed | 2024-03-08T23:31:58Z |
| publishDate | 2023-12-01 |
| publisher | West Bengal Veterinary Alumni Association |
| record_format | Article |
| series | Exploratory Animal and Medical Research |
| spelling | doaj.art-9bc99354dd25413b9f8126b81a771bb12023-12-14T13:20:17ZengWest Bengal Veterinary Alumni AssociationExploratory Animal and Medical Research2277-470X2319-247X2023-12-0113224325110.52635/eamr/13.2.243-251IN SILICO AND IN VITRO EVALUATION OF ENROFLOXACIN ON AFLATOXIN B1-INDUCED CYTOTOXICITYT. Jagadeesh 0M. Parthiban 1P. Raja2 Ghadevaru Sarathchandra 3S. Vairamuthu4 Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India. Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India. Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India. Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India. Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India.Additional therapeutics are required to minimize the toxicity of aflatoxin B1 (AFB1) in animals due to the drawbacks of mycotoxin binders. The cytochrome P450 enzyme system is necessary for the metabolic activation of AFB1 before it produces the cytotoxic AFB1-exo-8, 9-epoxide (AFBO). It is already proven that enrofloxacin (ENR) has been shown to substantially decrease the activity of the cytochrome P450 enzymes. To understand how ENR affects AFB1-induced cytotoxicity, it is important to highlight this context. In this present study, molecular docking was performed between ENR with CYP3A4 protein, apoptotic proteins (Bax, caspase 3, caspase 8, fas L, MAPK1), and catalase. To assess the effect of ENR on AFB1-induced cytotoxicity by MTT assay and trypan blue dye exclusion techniques, the mammalian simulative Vero cell lines were used in different treatment groups as AFB1 alone, ENR alone, AFB1+ENR, and AFB1+silymarin (known cell protective agent). This was done to further evaluate the in silico analysis. ENR interacted more positively with all of the proteins (CYP3A4, Bax, catalase, caspase 3, caspase 8, MAPK1, and fas L), according to the findings of molecular docking studies. On the other hand, cytotoxicity caused by AFB1 is successfully prevented by ENR at a dose of 25 µg/mL. The results of the present study suggest that ENR primarily protects against cytotoxicity induced by AFB1 in Vero cells as the evidence of in silico studies revealed that ENR may protect AFB1-induced cytotoxicity by interacting with the CYP3A4 enzyme which is primarily required for activation AFB1.https://animalmedicalresearch.org/Vol.13_Issue-2_December_2023/In%20silico%20and%20in%20vitro%20evaluationof%20enrofloxacin%20on%20aflatoxin%20B1-induced%20cytotoxicity.pdfaflatoxin b1enrofloxacinmolecular dockingapoptotic proteinsin vitro study |
| spellingShingle | T. Jagadeesh M. Parthiban P. Raja Ghadevaru Sarathchandra S. Vairamuthu IN SILICO AND IN VITRO EVALUATION OF ENROFLOXACIN ON AFLATOXIN B1-INDUCED CYTOTOXICITY Exploratory Animal and Medical Research aflatoxin b1 enrofloxacin molecular docking apoptotic proteins in vitro study |
| title | IN SILICO AND IN VITRO EVALUATION OF ENROFLOXACIN ON AFLATOXIN B1-INDUCED CYTOTOXICITY |
| title_full | IN SILICO AND IN VITRO EVALUATION OF ENROFLOXACIN ON AFLATOXIN B1-INDUCED CYTOTOXICITY |
| title_fullStr | IN SILICO AND IN VITRO EVALUATION OF ENROFLOXACIN ON AFLATOXIN B1-INDUCED CYTOTOXICITY |
| title_full_unstemmed | IN SILICO AND IN VITRO EVALUATION OF ENROFLOXACIN ON AFLATOXIN B1-INDUCED CYTOTOXICITY |
| title_short | IN SILICO AND IN VITRO EVALUATION OF ENROFLOXACIN ON AFLATOXIN B1-INDUCED CYTOTOXICITY |
| title_sort | in silico and in vitro evaluation of enrofloxacin on aflatoxin b1 induced cytotoxicity |
| topic | aflatoxin b1 enrofloxacin molecular docking apoptotic proteins in vitro study |
| url | https://animalmedicalresearch.org/Vol.13_Issue-2_December_2023/In%20silico%20and%20in%20vitro%20evaluationof%20enrofloxacin%20on%20aflatoxin%20B1-induced%20cytotoxicity.pdf |
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