Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
Abstract: Aim This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP)...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Limnologia
2022-10-01
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| Series: | Acta Limnologica Brasiliensia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2022000100720&tlng=en |
| _version_ | 1811182074429702144 |
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| author | Isabel Ballesteros Sara de la Cruz Michelle Rojas Génesis Salazar Miguel Martínez-Fresneda Pablo Castillejo |
| author_facet | Isabel Ballesteros Sara de la Cruz Michelle Rojas Génesis Salazar Miguel Martínez-Fresneda Pablo Castillejo |
| author_sort | Isabel Ballesteros |
| collection | DOAJ |
| description | Abstract: Aim This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies. |
| first_indexed | 2024-04-11T09:27:07Z |
| format | Article |
| id | doaj.art-9bcb1e7cd62d4772b581cbf876aa8764 |
| institution | Directory Open Access Journal |
| issn | 2179-975X |
| language | English |
| last_indexed | 2024-04-11T09:27:07Z |
| publishDate | 2022-10-01 |
| publisher | Associação Brasileira de Limnologia |
| record_format | Article |
| series | Acta Limnologica Brasiliensia |
| spelling | doaj.art-9bcb1e7cd62d4772b581cbf876aa87642022-12-22T04:32:00ZengAssociação Brasileira de LimnologiaActa Limnologica Brasiliensia2179-975X2022-10-013410.1590/s2179-975x2122Screening of cyanotoxin producing genes in Ecuadorian freshwater systemsIsabel Ballesteroshttps://orcid.org/0000-0001-6633-3607Sara de la CruzMichelle RojasGénesis SalazarMiguel Martínez-Fresnedahttps://orcid.org/0000-0001-9595-8067Pablo Castillejohttps://orcid.org/0000-0001-9806-0877Abstract: Aim This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2022000100720&tlng=encylindrospermopsinmicrocystinnested PCRAndesAmazon |
| spellingShingle | Isabel Ballesteros Sara de la Cruz Michelle Rojas Génesis Salazar Miguel Martínez-Fresneda Pablo Castillejo Screening of cyanotoxin producing genes in Ecuadorian freshwater systems Acta Limnologica Brasiliensia cylindrospermopsin microcystin nested PCR Andes Amazon |
| title | Screening of cyanotoxin producing genes in Ecuadorian freshwater systems |
| title_full | Screening of cyanotoxin producing genes in Ecuadorian freshwater systems |
| title_fullStr | Screening of cyanotoxin producing genes in Ecuadorian freshwater systems |
| title_full_unstemmed | Screening of cyanotoxin producing genes in Ecuadorian freshwater systems |
| title_short | Screening of cyanotoxin producing genes in Ecuadorian freshwater systems |
| title_sort | screening of cyanotoxin producing genes in ecuadorian freshwater systems |
| topic | cylindrospermopsin microcystin nested PCR Andes Amazon |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2022000100720&tlng=en |
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