29 Cardio-Omentopexy to Reduce Myocardial Scarring and Promote Regeneration
OBJECTIVES/GOALS: While the current management of single ventricle repairs has drastically prolonged life expectancy, the repair fails over time primarily through pathologic inflammation and fibrosis. Our goal is to demonstrate that cardio-omentopexy can decrease inflammation and fibrosis in swine a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2024-04-01
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| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866124000463/type/journal_article |
| _version_ | 1797226899996409856 |
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| author | Stephen Stachnik Dawn Parsell Joseph Forbess Kristopher Deatrick |
| author_facet | Stephen Stachnik Dawn Parsell Joseph Forbess Kristopher Deatrick |
| author_sort | Stephen Stachnik |
| collection | DOAJ |
| description | OBJECTIVES/GOALS: While the current management of single ventricle repairs has drastically prolonged life expectancy, the repair fails over time primarily through pathologic inflammation and fibrosis. Our goal is to demonstrate that cardio-omentopexy can decrease inflammation and fibrosis in swine after cryoinjury. METHODS/STUDY POPULATION: A cryoinjury is created using a liquid nitrogen cooled probe to the right ventricle of 15-20kg swine for three minutes. In half the groups the omentum is attached to the heart over the area of the injury. The swine are recovered and monitored for 4 or 8 weeks at which time they are euthanized. The injured area is evaluated via histological and immunohistochemical testing for markers of inflammation and scarring including collagen type, scar area, macrophage activity. RESULTS/ANTICIPATED RESULTS: Currently, we have successfully validated the animal model to create myocardial scar validated by histological testing. We anticipate that the addition of omentopexy to cryoinjury will decrease scar area, fibrosis and markers of chronic inflammation. Additionally, we expect an increase in myocytes in the area of injury. We expect that this will occur through the anti-inflammatory and protective mechanism of the omentum. DISCUSSION/SIGNIFICANCE: Cardio-omentopexy, if able to decrease fibrosis and preserve myocytes, may provide a useful adjunct to the treatment of single ventricle repair by prolonging the longevity of the repair. Additionally, as these repairs often require a ventriculotomy, decreasing the operative scar may preserve myocardial function. |
| first_indexed | 2024-04-24T14:32:15Z |
| format | Article |
| id | doaj.art-9bcc9343ff53494d9b3a2f3b4234de3b |
| institution | Directory Open Access Journal |
| issn | 2059-8661 |
| language | English |
| last_indexed | 2024-04-24T14:32:15Z |
| publishDate | 2024-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj.art-9bcc9343ff53494d9b3a2f3b4234de3b2024-04-03T02:00:11ZengCambridge University PressJournal of Clinical and Translational Science2059-86612024-04-0188810.1017/cts.2024.4629 Cardio-Omentopexy to Reduce Myocardial Scarring and Promote RegenerationStephen Stachnik0Dawn Parsell1Joseph Forbess2Kristopher Deatrick3University of Maryland Medical CenterUniversity of Maryland Medical CenterUniversity of Maryland Medical CenterUniversity of Maryland Medical CenterOBJECTIVES/GOALS: While the current management of single ventricle repairs has drastically prolonged life expectancy, the repair fails over time primarily through pathologic inflammation and fibrosis. Our goal is to demonstrate that cardio-omentopexy can decrease inflammation and fibrosis in swine after cryoinjury. METHODS/STUDY POPULATION: A cryoinjury is created using a liquid nitrogen cooled probe to the right ventricle of 15-20kg swine for three minutes. In half the groups the omentum is attached to the heart over the area of the injury. The swine are recovered and monitored for 4 or 8 weeks at which time they are euthanized. The injured area is evaluated via histological and immunohistochemical testing for markers of inflammation and scarring including collagen type, scar area, macrophage activity. RESULTS/ANTICIPATED RESULTS: Currently, we have successfully validated the animal model to create myocardial scar validated by histological testing. We anticipate that the addition of omentopexy to cryoinjury will decrease scar area, fibrosis and markers of chronic inflammation. Additionally, we expect an increase in myocytes in the area of injury. We expect that this will occur through the anti-inflammatory and protective mechanism of the omentum. DISCUSSION/SIGNIFICANCE: Cardio-omentopexy, if able to decrease fibrosis and preserve myocytes, may provide a useful adjunct to the treatment of single ventricle repair by prolonging the longevity of the repair. Additionally, as these repairs often require a ventriculotomy, decreasing the operative scar may preserve myocardial function.https://www.cambridge.org/core/product/identifier/S2059866124000463/type/journal_article |
| spellingShingle | Stephen Stachnik Dawn Parsell Joseph Forbess Kristopher Deatrick 29 Cardio-Omentopexy to Reduce Myocardial Scarring and Promote Regeneration Journal of Clinical and Translational Science |
| title | 29 Cardio-Omentopexy to Reduce Myocardial Scarring and Promote Regeneration |
| title_full | 29 Cardio-Omentopexy to Reduce Myocardial Scarring and Promote Regeneration |
| title_fullStr | 29 Cardio-Omentopexy to Reduce Myocardial Scarring and Promote Regeneration |
| title_full_unstemmed | 29 Cardio-Omentopexy to Reduce Myocardial Scarring and Promote Regeneration |
| title_short | 29 Cardio-Omentopexy to Reduce Myocardial Scarring and Promote Regeneration |
| title_sort | 29 cardio omentopexy to reduce myocardial scarring and promote regeneration |
| url | https://www.cambridge.org/core/product/identifier/S2059866124000463/type/journal_article |
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