Challenges of in vitro conservation of Сitrus germplasm resources
The main problems of establishment a slow growth in vitro collection of citrus and other tree crops cultivars are high degree of fungal contamination of bud explants and low growth potential of shoots. In this regard, the aim of current research is to assess the efficiency of decontamination procedu...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-02-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/1864 |
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| author | V. M. Gorshkov L. S. Samarina R. V. Kulyan V. I. Malyarovskaya A. V. Ryndin R. S. Rakhmangulov Y. L. Orlov |
| author_facet | V. M. Gorshkov L. S. Samarina R. V. Kulyan V. I. Malyarovskaya A. V. Ryndin R. S. Rakhmangulov Y. L. Orlov |
| author_sort | V. M. Gorshkov |
| collection | DOAJ |
| description | The main problems of establishment a slow growth in vitro collection of citrus and other tree crops cultivars are high degree of fungal contamination of bud explants and low growth potential of shoots. In this regard, the aim of current research is to assess the efficiency of decontamination procedure and the possibility of tissue culture initiation and slow growth conservation of valuable lemon cultivars. The best results of surface sterilization were obtained using immersion solutions of 0.3 % Veltolen – 25 minutes or 10 % Domestos – 25–30 minutes. In these treatments, 27.7–33.0 % of aseptic explants were obtained, respectively. However, after the third subculture, the yield of aseptic viable explants decreased till 10 % as a result of secondary contamination by endophytic fungi. The addition of biocide (“Gavrish”) in a nutrient medium at a concentration of 1 ml/l helped to increase the yield of aseptic viable explants till 50 %. However, after the third subculture the photosynthetic activity and the pigments content as well as growth rate decreased. Plants dropped yellowish leaves and eventually died. Thus, 37.35 % of plantlets survived after 8 months of conservation, and only 14.6 % survived after 10 months. Even after the third month of conservation significant decrease in the viability index and the coefficient of photosynthetic activity occurred in plants. Chlorophyll a in leaves decreased from 1.59 to 1.14 mg/g during 12 months in vitro conservation. The similar tendency observed on clorophyll b and carotenoids content. The experiments were carried out for 5 years using different lemon cultivars and other citrus varieties and cultivars. Thus, micropropagation and slow growth in vitro conservation of valuable lemon cultivars are still problematic and requires new technical solutions due to the low growth potential of plantlets raised from the mature buds that is consistent with the data of other researchers. |
| first_indexed | 2024-03-07T16:06:15Z |
| format | Article |
| id | doaj.art-9bcd0080b8a541079bf31b1b7233a2d5 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:08:43Z |
| publishDate | 2019-02-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-9bcd0080b8a541079bf31b1b7233a2d52024-04-11T15:31:00ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-02-01231242810.18699/VJ19.457867Challenges of in vitro conservation of Сitrus germplasm resourcesV. M. Gorshkov0L. S. Samarina1R. V. Kulyan2V. I. Malyarovskaya3A. V. Ryndin4R. S. Rakhmangulov5Y. L. Orlov6Russian Research Institute of Floriculture and Subtropical CropsRussian Research Institute of Floriculture and Subtropical CropsRussian Research Institute of Floriculture and Subtropical CropsRussian Research Institute of Floriculture and Subtropical CropsRussian Research Institute of Floriculture and Subtropical CropsRussian Research Institute of Floriculture and Subtropical CropsInstitute of Cytology and Genetics, SB RASThe main problems of establishment a slow growth in vitro collection of citrus and other tree crops cultivars are high degree of fungal contamination of bud explants and low growth potential of shoots. In this regard, the aim of current research is to assess the efficiency of decontamination procedure and the possibility of tissue culture initiation and slow growth conservation of valuable lemon cultivars. The best results of surface sterilization were obtained using immersion solutions of 0.3 % Veltolen – 25 minutes or 10 % Domestos – 25–30 minutes. In these treatments, 27.7–33.0 % of aseptic explants were obtained, respectively. However, after the third subculture, the yield of aseptic viable explants decreased till 10 % as a result of secondary contamination by endophytic fungi. The addition of biocide (“Gavrish”) in a nutrient medium at a concentration of 1 ml/l helped to increase the yield of aseptic viable explants till 50 %. However, after the third subculture the photosynthetic activity and the pigments content as well as growth rate decreased. Plants dropped yellowish leaves and eventually died. Thus, 37.35 % of plantlets survived after 8 months of conservation, and only 14.6 % survived after 10 months. Even after the third month of conservation significant decrease in the viability index and the coefficient of photosynthetic activity occurred in plants. Chlorophyll a in leaves decreased from 1.59 to 1.14 mg/g during 12 months in vitro conservation. The similar tendency observed on clorophyll b and carotenoids content. The experiments were carried out for 5 years using different lemon cultivars and other citrus varieties and cultivars. Thus, micropropagation and slow growth in vitro conservation of valuable lemon cultivars are still problematic and requires new technical solutions due to the low growth potential of plantlets raised from the mature buds that is consistent with the data of other researchers.https://vavilov.elpub.ru/jour/article/view/1864citrusnodal explantcontaminationmicropropagationslow growth conservationculture mediatrueto-typeendophytes |
| spellingShingle | V. M. Gorshkov L. S. Samarina R. V. Kulyan V. I. Malyarovskaya A. V. Ryndin R. S. Rakhmangulov Y. L. Orlov Challenges of in vitro conservation of Сitrus germplasm resources Вавиловский журнал генетики и селекции citrus nodal explant contamination micropropagation slow growth conservation culture media trueto-type endophytes |
| title | Challenges of in vitro conservation of Сitrus germplasm resources |
| title_full | Challenges of in vitro conservation of Сitrus germplasm resources |
| title_fullStr | Challenges of in vitro conservation of Сitrus germplasm resources |
| title_full_unstemmed | Challenges of in vitro conservation of Сitrus germplasm resources |
| title_short | Challenges of in vitro conservation of Сitrus germplasm resources |
| title_sort | challenges of in vitro conservation of сitrus germplasm resources |
| topic | citrus nodal explant contamination micropropagation slow growth conservation culture media trueto-type endophytes |
| url | https://vavilov.elpub.ru/jour/article/view/1864 |
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