Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
<p>Abstract</p> <p>We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68<sup>Gag </sup>moi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2005-07-01
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| Series: | Retrovirology |
| Online Access: | http://www.retrovirology.com/content/2/1/44 |
| _version_ | 1818492260126294016 |
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| author | Herchenröder Ottmar Rudolph Wolfram Cartellieri Marc Lindemann Dirk Rethwilm Axel |
| author_facet | Herchenröder Ottmar Rudolph Wolfram Cartellieri Marc Lindemann Dirk Rethwilm Axel |
| author_sort | Herchenröder Ottmar |
| collection | DOAJ |
| description | <p>Abstract</p> <p>We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68<sup>Gag </sup>moiety dominates in infectious FVs. Furthermore, approximate mean ratios in FV are 16:1 (pr71<sup>Gag </sup>plus p68<sup>Gag</sup>:p85<sup>RT</sup>),12:1 (p68<sup>Gag</sup>:p85<sup>RT</sup>), and 10:1 (pr71<sup>Gag </sup>plus p68<sup>Gag</sup>:p40<sup>IN</sup>). Thus, the results indicate that FVs have found a way to incorporate approximately as much Pol protein into their capsids as orthoretroviruses, despite a completely different Pol expression strategy.</p> |
| first_indexed | 2024-12-10T17:40:42Z |
| format | Article |
| id | doaj.art-9bcd536df146424ab74a26fa8084c8c0 |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-10T17:40:42Z |
| publishDate | 2005-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-9bcd536df146424ab74a26fa8084c8c02022-12-22T01:39:23ZengBMCRetrovirology1742-46902005-07-01214410.1186/1742-4690-2-44Determination of the relative amounts of Gag and Pol proteins in foamy virus particlesHerchenröder OttmarRudolph WolframCartellieri MarcLindemann DirkRethwilm Axel<p>Abstract</p> <p>We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68<sup>Gag </sup>moiety dominates in infectious FVs. Furthermore, approximate mean ratios in FV are 16:1 (pr71<sup>Gag </sup>plus p68<sup>Gag</sup>:p85<sup>RT</sup>),12:1 (p68<sup>Gag</sup>:p85<sup>RT</sup>), and 10:1 (pr71<sup>Gag </sup>plus p68<sup>Gag</sup>:p40<sup>IN</sup>). Thus, the results indicate that FVs have found a way to incorporate approximately as much Pol protein into their capsids as orthoretroviruses, despite a completely different Pol expression strategy.</p>http://www.retrovirology.com/content/2/1/44 |
| spellingShingle | Herchenröder Ottmar Rudolph Wolfram Cartellieri Marc Lindemann Dirk Rethwilm Axel Determination of the relative amounts of Gag and Pol proteins in foamy virus particles Retrovirology |
| title | Determination of the relative amounts of Gag and Pol proteins in foamy virus particles |
| title_full | Determination of the relative amounts of Gag and Pol proteins in foamy virus particles |
| title_fullStr | Determination of the relative amounts of Gag and Pol proteins in foamy virus particles |
| title_full_unstemmed | Determination of the relative amounts of Gag and Pol proteins in foamy virus particles |
| title_short | Determination of the relative amounts of Gag and Pol proteins in foamy virus particles |
| title_sort | determination of the relative amounts of gag and pol proteins in foamy virus particles |
| url | http://www.retrovirology.com/content/2/1/44 |
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