Assessment of cardiovascular fibrosis using novel fluorescent probes.
Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-04-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3080412?pdf=render |
| _version_ | 1818585567563087872 |
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| author | Jiqiu Chen Seung Koo Lee Wael R Abd-Elgaliel Lifan Liang Elisa-Yaniz Galende Roger J Hajjar Ching-Hsuan Tung |
| author_facet | Jiqiu Chen Seung Koo Lee Wael R Abd-Elgaliel Lifan Liang Elisa-Yaniz Galende Roger J Hajjar Ching-Hsuan Tung |
| author_sort | Jiqiu Chen |
| collection | DOAJ |
| description | Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells. |
| first_indexed | 2024-12-16T08:39:07Z |
| format | Article |
| id | doaj.art-9bce978368d34419ba86cf9ff8fcdb9d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:39:07Z |
| publishDate | 2011-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9bce978368d34419ba86cf9ff8fcdb9d2022-12-21T22:37:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-04-0164e1909710.1371/journal.pone.0019097Assessment of cardiovascular fibrosis using novel fluorescent probes.Jiqiu ChenSeung Koo LeeWael R Abd-ElgalielLifan LiangElisa-Yaniz GalendeRoger J HajjarChing-Hsuan TungCardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells.http://europepmc.org/articles/PMC3080412?pdf=render |
| spellingShingle | Jiqiu Chen Seung Koo Lee Wael R Abd-Elgaliel Lifan Liang Elisa-Yaniz Galende Roger J Hajjar Ching-Hsuan Tung Assessment of cardiovascular fibrosis using novel fluorescent probes. PLoS ONE |
| title | Assessment of cardiovascular fibrosis using novel fluorescent probes. |
| title_full | Assessment of cardiovascular fibrosis using novel fluorescent probes. |
| title_fullStr | Assessment of cardiovascular fibrosis using novel fluorescent probes. |
| title_full_unstemmed | Assessment of cardiovascular fibrosis using novel fluorescent probes. |
| title_short | Assessment of cardiovascular fibrosis using novel fluorescent probes. |
| title_sort | assessment of cardiovascular fibrosis using novel fluorescent probes |
| url | http://europepmc.org/articles/PMC3080412?pdf=render |
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