The whole body transcriptome of Coleophora obducta reveals important olfactory proteins
Background The tiny casebearer moth Coleophora obducta, an important defoliator of Larix spp., is a major threat to ecological security in north China. Studies have shown that C. obducta is strongly specific to host plants; it is unable complete its life cycle without Larix spp. The sex pheromones o...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2020-04-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8902.pdf |
| _version_ | 1827606094785019904 |
|---|---|
| author | Dongbai Wang Jing Tao Pengfei Lu Youqing Luo Ping Hu |
| author_facet | Dongbai Wang Jing Tao Pengfei Lu Youqing Luo Ping Hu |
| author_sort | Dongbai Wang |
| collection | DOAJ |
| description | Background The tiny casebearer moth Coleophora obducta, an important defoliator of Larix spp., is a major threat to ecological security in north China. Studies have shown that C. obducta is strongly specific to host plants; it is unable complete its life cycle without Larix spp. The sex pheromones of C. obducta Z5-10:OH have been elucidated; and eight types of antennae sensilla, have been detected, indicating that an exploration of its olfactory proteins is necessary, due to the general lack of information on this topic. Methods We investigated the whole body transcriptome of C. obducta, performed a phylogenetic analysis of its olfactory proteins and produced expression profiles of three pheromone-binding proteins (CobdPBPs) by qRT–PCR. Results We identified 16 odorant binding proteins, 14 chemosensory proteins, three sensory neuron membrane proteins, six odorant degrading enzymes, five antennal esterases, 13 odorant receptors, seven ionotropic receptors and 10 gustatory receptors, including three PBPs and one odorant co-receptor. Additionally, three putative pheromone receptors, two bitter gustatory receptors and five functional ionotropic receptors were found by phylogenetic analysis. The expression profiles of three PBPs in males and females showed that all of them exhibited male-specific expression and two were expressed at significantly higher levels in males. These data provide a molecular foundation from which to explore the olfactory recognition process and may be useful in the development of a new integrated pest management strategy targeting olfactory recognition of C. obducta. |
| first_indexed | 2024-03-09T06:32:11Z |
| format | Article |
| id | doaj.art-9bd2b19597234de0bf4f249e257a7aca |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:32:11Z |
| publishDate | 2020-04-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-9bd2b19597234de0bf4f249e257a7aca2023-12-03T11:03:21ZengPeerJ Inc.PeerJ2167-83592020-04-018e890210.7717/peerj.8902The whole body transcriptome of Coleophora obducta reveals important olfactory proteinsDongbai Wang0Jing Tao1Pengfei Lu2Youqing Luo3Ping Hu4Forestry College, Guangxi University, Nanning, Guangxi, ChinaBeijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, ChinaBeijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, ChinaBeijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, ChinaForestry College, Guangxi University, Nanning, Guangxi, ChinaBackground The tiny casebearer moth Coleophora obducta, an important defoliator of Larix spp., is a major threat to ecological security in north China. Studies have shown that C. obducta is strongly specific to host plants; it is unable complete its life cycle without Larix spp. The sex pheromones of C. obducta Z5-10:OH have been elucidated; and eight types of antennae sensilla, have been detected, indicating that an exploration of its olfactory proteins is necessary, due to the general lack of information on this topic. Methods We investigated the whole body transcriptome of C. obducta, performed a phylogenetic analysis of its olfactory proteins and produced expression profiles of three pheromone-binding proteins (CobdPBPs) by qRT–PCR. Results We identified 16 odorant binding proteins, 14 chemosensory proteins, three sensory neuron membrane proteins, six odorant degrading enzymes, five antennal esterases, 13 odorant receptors, seven ionotropic receptors and 10 gustatory receptors, including three PBPs and one odorant co-receptor. Additionally, three putative pheromone receptors, two bitter gustatory receptors and five functional ionotropic receptors were found by phylogenetic analysis. The expression profiles of three PBPs in males and females showed that all of them exhibited male-specific expression and two were expressed at significantly higher levels in males. These data provide a molecular foundation from which to explore the olfactory recognition process and may be useful in the development of a new integrated pest management strategy targeting olfactory recognition of C. obducta.https://peerj.com/articles/8902.pdfOdorant binding proteinsChemosensory proteinsOdorant receptorsIonotropic receptorsOdorant-degrading enzymesSensory neuron membrane proteins |
| spellingShingle | Dongbai Wang Jing Tao Pengfei Lu Youqing Luo Ping Hu The whole body transcriptome of Coleophora obducta reveals important olfactory proteins PeerJ Odorant binding proteins Chemosensory proteins Odorant receptors Ionotropic receptors Odorant-degrading enzymes Sensory neuron membrane proteins |
| title | The whole body transcriptome of Coleophora obducta reveals important olfactory proteins |
| title_full | The whole body transcriptome of Coleophora obducta reveals important olfactory proteins |
| title_fullStr | The whole body transcriptome of Coleophora obducta reveals important olfactory proteins |
| title_full_unstemmed | The whole body transcriptome of Coleophora obducta reveals important olfactory proteins |
| title_short | The whole body transcriptome of Coleophora obducta reveals important olfactory proteins |
| title_sort | whole body transcriptome of coleophora obducta reveals important olfactory proteins |
| topic | Odorant binding proteins Chemosensory proteins Odorant receptors Ionotropic receptors Odorant-degrading enzymes Sensory neuron membrane proteins |
| url | https://peerj.com/articles/8902.pdf |
| work_keys_str_mv | AT dongbaiwang thewholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT jingtao thewholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT pengfeilu thewholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT youqingluo thewholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT pinghu thewholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT dongbaiwang wholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT jingtao wholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT pengfeilu wholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT youqingluo wholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins AT pinghu wholebodytranscriptomeofcoleophoraobductarevealsimportantolfactoryproteins |