Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model
The present study explores the effects of two supplementation levels of Debaryomyces hansenii (1.1% and 2.2%) as a probiotic in a reference low fish meal-based diet on the skin mucosal tissue in Sparus aurata. This study includes the evaluation of fish performance coupled with a holistic study of th...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1247199/full |
| _version_ | 1797730964264189952 |
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| author | Ignasi Sanahuja Laura Fernandez-Alacid Silvia Torrecillas Alberto Ruiz Eva Vallejos-Vidal Joana P. Firmino Felipe E. Reyes-Lopez Lluis Tort Dariel Tovar-Ramirez Antoni Ibarz Enric Gisbert |
| author_facet | Ignasi Sanahuja Laura Fernandez-Alacid Silvia Torrecillas Alberto Ruiz Eva Vallejos-Vidal Joana P. Firmino Felipe E. Reyes-Lopez Lluis Tort Dariel Tovar-Ramirez Antoni Ibarz Enric Gisbert |
| author_sort | Ignasi Sanahuja |
| collection | DOAJ |
| description | The present study explores the effects of two supplementation levels of Debaryomyces hansenii (1.1% and 2.2%) as a probiotic in a reference low fish meal-based diet on the skin mucosal tissue in Sparus aurata. This study includes the evaluation of fish performance coupled with a holistic study of the skin mucosa: i) a transcriptomic study of the skin tissue, and ii) the evaluation of its secreted mucus both in terms of skin mucosal-associated biomarkers and its defensive capacity by means of co-culture analysis with two pathogenic bacteria. Results showed that after 70 days of diet administration, fish fed the diet supplemented with D. hansenii at 1.1% presented increased somatic growth and a better feed conversion ratio, compared to fish fed the control diet. In contrast, fish fed the diet including 2.2% of the probiotic presented intermediate values. Regarding gene regulation, the probiotic administration at 1.1% resulted in 712 differentially expressed genes (DEGs), among which 53.4% and 46.6% were up- and down-regulated, respectively. In particular, D. hansenii modulated some skin biological processes related to immunity and metabolism. Specifically, D. hansenii administration induced a strong modulation of some immune biological-related processes (61 DEGs), mainly involved in B- and T-cell regulatory pathways. Furthermore, dietary D. hansenii promoted the skin barrier function by the upregulation of anchoring junction genes (23 DEGs), which reinforces the physical defense against potential skin damage. In contrast, the skin showed modulated genes related to extracellular exosome and membrane organization (50 DEGs). This modulated functioning is of great interest, particularly in relation to the increased skin mucus defensive capacity observed in the bacterial co-culture in vitro trials, which could be related to the increased modulation and exudation of the innate immune components from the skin cells into the mucus. In summary, the modulation of innate immune parameters coupled with increased skin barrier function and cell trafficking potentiates the skin’s physical barrier and mucus defensive capacity, while maintaining the skin mucosa’s homeostatic immune and metabolic status. These findings confirmed the advantages of D. hansenii supplementation in low fish meal-based diets, demonstrating the probiotic benefits on cultured marine species. |
| first_indexed | 2024-03-12T11:51:45Z |
| format | Article |
| id | doaj.art-9bd40768382a4b1fbfab9d6f024a6ba7 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T11:51:45Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-9bd40768382a4b1fbfab9d6f024a6ba72023-08-31T06:44:24ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-08-011410.3389/fimmu.2023.12471991247199Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish modelIgnasi Sanahuja0Laura Fernandez-Alacid1Silvia Torrecillas2Alberto Ruiz3Eva Vallejos-Vidal4Joana P. Firmino5Felipe E. Reyes-Lopez6Lluis Tort7Dariel Tovar-Ramirez8Antoni Ibarz9Enric Gisbert10Aquaculture Program, Institute of Agrifood Research and Technology (IRTA), La Ràpita, SpainDepartment of Cell Biology, Physiology, and Immunology, University of Barcelona, Barcelona, SpainAquaculture Program, Institute of Agrifood Research and Technology (IRTA), La Ràpita, SpainAquaculture Program, Institute of Agrifood Research and Technology (IRTA), La Ràpita, SpainNúcleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agronómicas, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Santiago, ChileAquaculture Program, Institute of Agrifood Research and Technology (IRTA), La Ràpita, SpainCentro de Biotecnología Acuícola, Universidad de Santiago de Chile, Santiago, ChileDepartment of Cell Biology, Physiology, and Immunology, Universitat Autonoma de Barcelona, Barcelona, SpainCentro de Investigaciones Biológicas del Noroeste SC, CIBNOR, La Paz, MexicoDepartment of Cell Biology, Physiology, and Immunology, University of Barcelona, Barcelona, SpainAquaculture Program, Institute of Agrifood Research and Technology (IRTA), La Ràpita, SpainThe present study explores the effects of two supplementation levels of Debaryomyces hansenii (1.1% and 2.2%) as a probiotic in a reference low fish meal-based diet on the skin mucosal tissue in Sparus aurata. This study includes the evaluation of fish performance coupled with a holistic study of the skin mucosa: i) a transcriptomic study of the skin tissue, and ii) the evaluation of its secreted mucus both in terms of skin mucosal-associated biomarkers and its defensive capacity by means of co-culture analysis with two pathogenic bacteria. Results showed that after 70 days of diet administration, fish fed the diet supplemented with D. hansenii at 1.1% presented increased somatic growth and a better feed conversion ratio, compared to fish fed the control diet. In contrast, fish fed the diet including 2.2% of the probiotic presented intermediate values. Regarding gene regulation, the probiotic administration at 1.1% resulted in 712 differentially expressed genes (DEGs), among which 53.4% and 46.6% were up- and down-regulated, respectively. In particular, D. hansenii modulated some skin biological processes related to immunity and metabolism. Specifically, D. hansenii administration induced a strong modulation of some immune biological-related processes (61 DEGs), mainly involved in B- and T-cell regulatory pathways. Furthermore, dietary D. hansenii promoted the skin barrier function by the upregulation of anchoring junction genes (23 DEGs), which reinforces the physical defense against potential skin damage. In contrast, the skin showed modulated genes related to extracellular exosome and membrane organization (50 DEGs). This modulated functioning is of great interest, particularly in relation to the increased skin mucus defensive capacity observed in the bacterial co-culture in vitro trials, which could be related to the increased modulation and exudation of the innate immune components from the skin cells into the mucus. In summary, the modulation of innate immune parameters coupled with increased skin barrier function and cell trafficking potentiates the skin’s physical barrier and mucus defensive capacity, while maintaining the skin mucosa’s homeostatic immune and metabolic status. These findings confirmed the advantages of D. hansenii supplementation in low fish meal-based diets, demonstrating the probiotic benefits on cultured marine species.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1247199/fullDebaryomyces hanseniiskin defenseskin mucus defenseskin mucus associated biomarkerstranscriptomicsyeast probiotic |
| spellingShingle | Ignasi Sanahuja Laura Fernandez-Alacid Silvia Torrecillas Alberto Ruiz Eva Vallejos-Vidal Joana P. Firmino Felipe E. Reyes-Lopez Lluis Tort Dariel Tovar-Ramirez Antoni Ibarz Enric Gisbert Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model Frontiers in Immunology Debaryomyces hansenii skin defense skin mucus defense skin mucus associated biomarkers transcriptomics yeast probiotic |
| title | Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model |
| title_full | Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model |
| title_fullStr | Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model |
| title_full_unstemmed | Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model |
| title_short | Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model |
| title_sort | dietary debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model |
| topic | Debaryomyces hansenii skin defense skin mucus defense skin mucus associated biomarkers transcriptomics yeast probiotic |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1247199/full |
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