Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo
Natural antioxidants derived from plants have shown a tremendous inhibitory effect on free radicals in actively metabolizing cells. Overproduction of free radicals increases the risk factor of chronic diseases associated with diabetes, cancer, arthritis and cardiovascular disease. Andrographis panic...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Journal of Traditional and Complementary Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S222541101630308X |
| _version_ | 1818062071874453504 |
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| author | Hassan Fahmi Ismail Zanariah Hashim Wong Tet Soon Nur Syukriah Ab Rahman Ain Nabihah Zainudin Fadzilah Adibah Abdul Majid |
| author_facet | Hassan Fahmi Ismail Zanariah Hashim Wong Tet Soon Nur Syukriah Ab Rahman Ain Nabihah Zainudin Fadzilah Adibah Abdul Majid |
| author_sort | Hassan Fahmi Ismail |
| collection | DOAJ |
| description | Natural antioxidants derived from plants have shown a tremendous inhibitory effect on free radicals in actively metabolizing cells. Overproduction of free radicals increases the risk factor of chronic diseases associated with diabetes, cancer, arthritis and cardiovascular disease. Andrographis paniculata, Cinnamon zeylanicum, Curcuma xanthorrhiza, Eugenia polyantha and Orthosiphon stamineus are ethnomedicinal plants used in the Asian region to treat various illnesses from a common fever to metabolic disease. In this study, we have quantified the total phenolic (TPC) and flavonoid content (TFC) in these plants and its inhibitory effect on 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals as well as the cytotoxicity effect on cell lines proliferation and zebrafish embryogenesis. Results showed that Cinnamon zeylanicum and E. polyantha have the highest phenolic and flavonoid content. Furthermore, both herbs significantly inhibited the formation of DPPH and ABTS free radicals. Meanwhile, O. stamineus exhibited minimum cytotoxicity and embryotoxicity on tested models. Good correlation between IC50 of 3T3-L1 cells and LC50 embyrotoxicity was also found. This study revealed the potent activity of antioxidant against free radical and the toxicology levels of the tested herbal plants. |
| first_indexed | 2024-12-10T13:58:23Z |
| format | Article |
| id | doaj.art-9bd75fa6a8a244c6b9ff3509f54027d0 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-10T13:58:23Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-9bd75fa6a8a244c6b9ff3509f54027d02022-12-22T01:45:52ZengElsevierJournal of Traditional and Complementary Medicine2225-41102017-10-017445246510.1016/j.jtcme.2016.12.006Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryoHassan Fahmi Ismail0Zanariah Hashim1Wong Tet Soon2Nur Syukriah Ab Rahman3Ain Nabihah Zainudin4Fadzilah Adibah Abdul Majid5Department of Bioprocess and Polymer Engineering, Faculty of Chemical & Energy Engineering, Universiti Teknologi Malaysia, 81310, MalaysiaDepartment of Bioprocess and Polymer Engineering, Faculty of Chemical & Energy Engineering, Universiti Teknologi Malaysia, 81310, MalaysiaDepartment of Bioprocess and Polymer Engineering, Faculty of Chemical & Energy Engineering, Universiti Teknologi Malaysia, 81310, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, MalaysiaNatural antioxidants derived from plants have shown a tremendous inhibitory effect on free radicals in actively metabolizing cells. Overproduction of free radicals increases the risk factor of chronic diseases associated with diabetes, cancer, arthritis and cardiovascular disease. Andrographis paniculata, Cinnamon zeylanicum, Curcuma xanthorrhiza, Eugenia polyantha and Orthosiphon stamineus are ethnomedicinal plants used in the Asian region to treat various illnesses from a common fever to metabolic disease. In this study, we have quantified the total phenolic (TPC) and flavonoid content (TFC) in these plants and its inhibitory effect on 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals as well as the cytotoxicity effect on cell lines proliferation and zebrafish embryogenesis. Results showed that Cinnamon zeylanicum and E. polyantha have the highest phenolic and flavonoid content. Furthermore, both herbs significantly inhibited the formation of DPPH and ABTS free radicals. Meanwhile, O. stamineus exhibited minimum cytotoxicity and embryotoxicity on tested models. Good correlation between IC50 of 3T3-L1 cells and LC50 embyrotoxicity was also found. This study revealed the potent activity of antioxidant against free radical and the toxicology levels of the tested herbal plants.http://www.sciencedirect.com/science/article/pii/S222541101630308XHerbalCytotoxicityEmbryotoxicityTotal phenolic contentAntioxidant |
| spellingShingle | Hassan Fahmi Ismail Zanariah Hashim Wong Tet Soon Nur Syukriah Ab Rahman Ain Nabihah Zainudin Fadzilah Adibah Abdul Majid Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo Journal of Traditional and Complementary Medicine Herbal Cytotoxicity Embryotoxicity Total phenolic content Antioxidant |
| title | Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo |
| title_full | Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo |
| title_fullStr | Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo |
| title_full_unstemmed | Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo |
| title_short | Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo |
| title_sort | comparative study of herbal plants on the phenolic and flavonoid content antioxidant activities and toxicity on cells and zebrafish embryo |
| topic | Herbal Cytotoxicity Embryotoxicity Total phenolic content Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S222541101630308X |
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