Emergence of <i>Cfr</i>-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> (LA-MRSA) from Healthy Pigs in Portugal
Livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) ST398 is mainly found in Europe and North America, colonizing the nasal cavity of pigs. This study characterized the MRSA isolates recovered from pig nasal swabs (<i>n</i> = 171) by evaluating t...
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MDPI AG
2022-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/10/1439 |
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| author | Célia Leão Lurdes Clemente Maria Cara d’Anjo Teresa Albuquerque Ana Amaro |
| author_facet | Célia Leão Lurdes Clemente Maria Cara d’Anjo Teresa Albuquerque Ana Amaro |
| author_sort | Célia Leão |
| collection | DOAJ |
| description | Livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) ST398 is mainly found in Europe and North America, colonizing the nasal cavity of pigs. This study characterized the MRSA isolates recovered from pig nasal swabs (<i>n</i> = 171) by evaluating the antimicrobial susceptibility profile by broth microdilution and characterizing the genetic lineages by <i>spa</i>-typing. Three linezolid-resistant isolates were subjected to Whole-Genome Sequencing (WGS). All strains harbored the <i>mecA</i> gene and were resistant to tetracycline and susceptible to vancomycin. A high frequency of multidrug resistance (97.6%) was evidenced, with 55 different multidrug resistance profiles identified. The MRSA strains were found to belong to 17 <i>spa</i>-types, three being novel. The linezolid-resistant strains appeared to belong to the ST398 type, <i>spa</i>-type t011, and SCC<i>mec</i>_type_Vc and to harbor the <i>cfr</i>, <i>fex</i>A, <i>bla</i>Z, <i>mec</i>A, <i>tet</i>M, and <i>tet</i>K genes. The <i>cfr</i> gene was predicted to be carried in the plasmid, flanked by <i>IS</i>Sau9 and the transposon TnpR. MRSA from Portuguese fattening pigs present a high diversity of genetic lineages. The presence of <i>cfr</i>-positive LA-MRSA may represent a risk of transmission to humans, mainly to those in contact with livestock. |
| first_indexed | 2024-03-09T20:49:46Z |
| format | Article |
| id | doaj.art-9bdcdc804d59408b9f68e39586dd18bd |
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| language | English |
| last_indexed | 2024-03-09T20:49:46Z |
| publishDate | 2022-10-01 |
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| series | Antibiotics |
| spelling | doaj.art-9bdcdc804d59408b9f68e39586dd18bd2023-11-23T22:36:26ZengMDPI AGAntibiotics2079-63822022-10-011110143910.3390/antibiotics11101439Emergence of <i>Cfr</i>-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> (LA-MRSA) from Healthy Pigs in PortugalCélia Leão0Lurdes Clemente1Maria Cara d’Anjo2Teresa Albuquerque3Ana Amaro4Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), 2780-157 Oeiras, PortugalLaboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), 2780-157 Oeiras, PortugalDGAV-Portuguese National Authority for Animal Health, 1700-093 Lisbon, PortugalLaboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), 2780-157 Oeiras, PortugalLaboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), 2780-157 Oeiras, PortugalLivestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) ST398 is mainly found in Europe and North America, colonizing the nasal cavity of pigs. This study characterized the MRSA isolates recovered from pig nasal swabs (<i>n</i> = 171) by evaluating the antimicrobial susceptibility profile by broth microdilution and characterizing the genetic lineages by <i>spa</i>-typing. Three linezolid-resistant isolates were subjected to Whole-Genome Sequencing (WGS). All strains harbored the <i>mecA</i> gene and were resistant to tetracycline and susceptible to vancomycin. A high frequency of multidrug resistance (97.6%) was evidenced, with 55 different multidrug resistance profiles identified. The MRSA strains were found to belong to 17 <i>spa</i>-types, three being novel. The linezolid-resistant strains appeared to belong to the ST398 type, <i>spa</i>-type t011, and SCC<i>mec</i>_type_Vc and to harbor the <i>cfr</i>, <i>fex</i>A, <i>bla</i>Z, <i>mec</i>A, <i>tet</i>M, and <i>tet</i>K genes. The <i>cfr</i> gene was predicted to be carried in the plasmid, flanked by <i>IS</i>Sau9 and the transposon TnpR. MRSA from Portuguese fattening pigs present a high diversity of genetic lineages. The presence of <i>cfr</i>-positive LA-MRSA may represent a risk of transmission to humans, mainly to those in contact with livestock.https://www.mdpi.com/2079-6382/11/10/1439LA-MRSApigslinezolid resistance<i>cfr</i> geneWGSST398 |
| spellingShingle | Célia Leão Lurdes Clemente Maria Cara d’Anjo Teresa Albuquerque Ana Amaro Emergence of <i>Cfr</i>-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> (LA-MRSA) from Healthy Pigs in Portugal Antibiotics LA-MRSA pigs linezolid resistance <i>cfr</i> gene WGS ST398 |
| title | Emergence of <i>Cfr</i>-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> (LA-MRSA) from Healthy Pigs in Portugal |
| title_full | Emergence of <i>Cfr</i>-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> (LA-MRSA) from Healthy Pigs in Portugal |
| title_fullStr | Emergence of <i>Cfr</i>-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> (LA-MRSA) from Healthy Pigs in Portugal |
| title_full_unstemmed | Emergence of <i>Cfr</i>-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> (LA-MRSA) from Healthy Pigs in Portugal |
| title_short | Emergence of <i>Cfr</i>-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> (LA-MRSA) from Healthy Pigs in Portugal |
| title_sort | emergence of i cfr i mediated linezolid resistance among livestock associated methicillin resistant i staphylococcus aureus i la mrsa from healthy pigs in portugal |
| topic | LA-MRSA pigs linezolid resistance <i>cfr</i> gene WGS ST398 |
| url | https://www.mdpi.com/2079-6382/11/10/1439 |
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