Markers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort study
Abstract Background Gender-affirming hormone therapy has been hypothesized to reduce the patient’s reproductive potential in transmen, although the exact long-term effects on future fertility are unknown. Methods In this prospective cohort study we aimed to evaluate ovaries of 20 transmen by using h...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Molecular Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s10020-020-00214-x |
| _version_ | 1818506898501009408 |
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| author | Julian Marschalek Detlef Pietrowski Sabine Dekan Marie-Louise Marschalek Maximilian Brandstetter Johannes Ott |
| author_facet | Julian Marschalek Detlef Pietrowski Sabine Dekan Marie-Louise Marschalek Maximilian Brandstetter Johannes Ott |
| author_sort | Julian Marschalek |
| collection | DOAJ |
| description | Abstract Background Gender-affirming hormone therapy has been hypothesized to reduce the patient’s reproductive potential in transmen, although the exact long-term effects on future fertility are unknown. Methods In this prospective cohort study we aimed to evaluate ovaries of 20 transmen by using hormone serum levels, histomorphological analysis and fluorescence activated cells sorting (FACS) analysis – in order to assess the amount of vital cells. Results The median total number of follicles per field of view was 39 (IQR 12–122). Of all follicles (n = 1661), the vast majority was primordial (n = 1505, 90.6%), followed by primary (n = 76, 4.6%), abnormal (n = 63, 3.8%) and secondary follicles (n = 17, 1.0%). FACS analysis was available for 13 samples (65.0%) and the median frequency of vital cells was 87.5% (IQR, 77.7–95.4%). Both a higher age (p = 0.032) and a lower BMI (p = 0.003) were significantly associated with a higher frequency of vital cells. Conclusion The majority of ovarian cells after long-term androgen treatment were vital in FACS analysis and histomorphological evaluation revealed a normal cortical follicle distribution. These results are currently exploratory, but might be promising for issues on fertility preservation. Trial registration The study was approved by the ethics committee of the Medical University of Vienna (EK 2240/2016) and was retrospectively registered in the Current Controlled Trials Register (registration number NCT03649087 , date of registration: 28.08.2018). |
| first_indexed | 2024-12-10T22:10:55Z |
| format | Article |
| id | doaj.art-9bddcb926f1140d1b6be67607c0babde |
| institution | Directory Open Access Journal |
| issn | 1076-1551 1528-3658 |
| language | English |
| last_indexed | 2024-12-10T22:10:55Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Medicine |
| spelling | doaj.art-9bddcb926f1140d1b6be67607c0babde2022-12-22T01:31:37ZengBMCMolecular Medicine1076-15511528-36582020-09-012611910.1186/s10020-020-00214-xMarkers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort studyJulian Marschalek0Detlef Pietrowski1Sabine Dekan2Marie-Louise Marschalek3Maximilian Brandstetter4Johannes Ott5Department of Obstetrics and Gynecology, Clinical Division of Gynecologic Endocrinology and Reproductive Medicine, Medical University of ViennaDepartment of Obstetrics and Gynecology, Clinical Division of Gynecologic Endocrinology and Reproductive Medicine, Medical University of ViennaClinical Institute of Pathology, Medical University of ViennaDepartment of Obstetrics and Gynecology, Clinical Division of Gynecologic Endocrinology and Reproductive Medicine, Medical University of ViennaDepartment of Obstetrics and Gynecology, Clinical Division of Gynecologic Endocrinology and Reproductive Medicine, Medical University of ViennaDepartment of Obstetrics and Gynecology, Clinical Division of Gynecologic Endocrinology and Reproductive Medicine, Medical University of ViennaAbstract Background Gender-affirming hormone therapy has been hypothesized to reduce the patient’s reproductive potential in transmen, although the exact long-term effects on future fertility are unknown. Methods In this prospective cohort study we aimed to evaluate ovaries of 20 transmen by using hormone serum levels, histomorphological analysis and fluorescence activated cells sorting (FACS) analysis – in order to assess the amount of vital cells. Results The median total number of follicles per field of view was 39 (IQR 12–122). Of all follicles (n = 1661), the vast majority was primordial (n = 1505, 90.6%), followed by primary (n = 76, 4.6%), abnormal (n = 63, 3.8%) and secondary follicles (n = 17, 1.0%). FACS analysis was available for 13 samples (65.0%) and the median frequency of vital cells was 87.5% (IQR, 77.7–95.4%). Both a higher age (p = 0.032) and a lower BMI (p = 0.003) were significantly associated with a higher frequency of vital cells. Conclusion The majority of ovarian cells after long-term androgen treatment were vital in FACS analysis and histomorphological evaluation revealed a normal cortical follicle distribution. These results are currently exploratory, but might be promising for issues on fertility preservation. Trial registration The study was approved by the ethics committee of the Medical University of Vienna (EK 2240/2016) and was retrospectively registered in the Current Controlled Trials Register (registration number NCT03649087 , date of registration: 28.08.2018).http://link.springer.com/article/10.1186/s10020-020-00214-xTransgenderTransmenFertilityHistomorphologyFluorescence activated cells sorting (FACS) |
| spellingShingle | Julian Marschalek Detlef Pietrowski Sabine Dekan Marie-Louise Marschalek Maximilian Brandstetter Johannes Ott Markers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort study Molecular Medicine Transgender Transmen Fertility Histomorphology Fluorescence activated cells sorting (FACS) |
| title | Markers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort study |
| title_full | Markers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort study |
| title_fullStr | Markers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort study |
| title_full_unstemmed | Markers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort study |
| title_short | Markers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort study |
| title_sort | markers of vitality in ovaries of transmen after long term androgen treatment a prospective cohort study |
| topic | Transgender Transmen Fertility Histomorphology Fluorescence activated cells sorting (FACS) |
| url | http://link.springer.com/article/10.1186/s10020-020-00214-x |
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