Ruminal bacterial diversity of yaks (Bos grunniens) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries
This study was conducted to investigate the ruminal bacterial diversity of yaks (<em>Bos grunniens</em>) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries. A total of 18 healthy Sibu male yaks (118±17 kg body weight) were randomly assigned to e...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2015-11-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/3970 |
| _version_ | 1818309471364972544 |
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| author | Lei Fang Zhenming Zhou Liping Ren Fenghua Shi Muyou Can Shatuo Chai Qingxiang Meng |
| author_facet | Lei Fang Zhenming Zhou Liping Ren Fenghua Shi Muyou Can Shatuo Chai Qingxiang Meng |
| author_sort | Lei Fang |
| collection | DOAJ |
| description | This study was conducted to investigate the ruminal bacterial diversity of yaks (<em>Bos grunniens</em>) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries. A total of 18 healthy Sibu male yaks (118±17 kg body weight) were randomly assigned to either indoor-feeding group (A) or grazing group (B). At the end of the 135-day experiment, ruminal bacterial diversity was analysed by using 16S rRNA gene clone libraries. A total of 212 clones were obtained from two libraries, divided into 162 operational taxonomic units (OTUs). Two libraries shared 13 OTUs. <em>Ruminococcus albus</em>, <em>Ruminococcus flavefaciens</em> and <em>Butyrivibrio fibrisolvens</em> were detected in both libraries. Whereas, <em>Selenomonas ruminantium</em>, <em>Succiniclasticum ruminis</em>, and <em>Butyrivibrio proteoclasticus</em> emerged only in library B, and <em>Pseudo-butyrivibrio ruminis</em> and <em>Roseburia faecis</em> appeared only in library A. A total of 73 out of 86 OTUs (84.88%) in library A and 65 out of 89 OTUs (73.03%) in library B fell into the phyla of low G+C subdivision, the remaining clones mainly belonged to the cytophaga/flexibacteria/ bacteroites subdivision in the two different libraries. In conclusion, different feeding patterns (indoor-feeding or grazing) had an effect on rumen bacterial diversity in yaks. |
| first_indexed | 2024-12-13T07:30:42Z |
| format | Article |
| id | doaj.art-9bde1ee019d54052b118e68ff50ba635 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-12-13T07:30:42Z |
| publishDate | 2015-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-9bde1ee019d54052b118e68ff50ba6352022-12-21T23:55:12ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2015-11-0114410.4081/ijas.2015.39702418Ruminal bacterial diversity of yaks (Bos grunniens) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene librariesLei Fang0Zhenming Zhou1Liping Ren2Fenghua Shi3Muyou Can4Shatuo Chai5Qingxiang Meng6College of Animal Science and Technology, China Agricultural University, Beijing; College of Animal Science, Tarim University, AlarCollege of Animal Science and Technology, China Agricultural University, BeijingCollege of Animal Science and Technology, China Agricultural University, BeijingCollege of Animal Science and Technology, China Agricultural University, BeijingTibet Academy of Agricultural and Livestock Science, LhasaAcademy of Animal Husbandry and Veterinary Medicine, Qinghai University, XiningCollege of Animal Science and Technology, China Agricultural University, BeijingThis study was conducted to investigate the ruminal bacterial diversity of yaks (<em>Bos grunniens</em>) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries. A total of 18 healthy Sibu male yaks (118±17 kg body weight) were randomly assigned to either indoor-feeding group (A) or grazing group (B). At the end of the 135-day experiment, ruminal bacterial diversity was analysed by using 16S rRNA gene clone libraries. A total of 212 clones were obtained from two libraries, divided into 162 operational taxonomic units (OTUs). Two libraries shared 13 OTUs. <em>Ruminococcus albus</em>, <em>Ruminococcus flavefaciens</em> and <em>Butyrivibrio fibrisolvens</em> were detected in both libraries. Whereas, <em>Selenomonas ruminantium</em>, <em>Succiniclasticum ruminis</em>, and <em>Butyrivibrio proteoclasticus</em> emerged only in library B, and <em>Pseudo-butyrivibrio ruminis</em> and <em>Roseburia faecis</em> appeared only in library A. A total of 73 out of 86 OTUs (84.88%) in library A and 65 out of 89 OTUs (73.03%) in library B fell into the phyla of low G+C subdivision, the remaining clones mainly belonged to the cytophaga/flexibacteria/ bacteroites subdivision in the two different libraries. In conclusion, different feeding patterns (indoor-feeding or grazing) had an effect on rumen bacterial diversity in yaks.http://www.aspajournal.it/index.php/ijas/article/view/3970Grazing yakIndoor-feeding yakRumen bacteria diversity16S rRNA gene |
| spellingShingle | Lei Fang Zhenming Zhou Liping Ren Fenghua Shi Muyou Can Shatuo Chai Qingxiang Meng Ruminal bacterial diversity of yaks (Bos grunniens) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries Italian Journal of Animal Science Grazing yak Indoor-feeding yak Rumen bacteria diversity 16S rRNA gene |
| title | Ruminal bacterial diversity of yaks (Bos grunniens) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries |
| title_full | Ruminal bacterial diversity of yaks (Bos grunniens) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries |
| title_fullStr | Ruminal bacterial diversity of yaks (Bos grunniens) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries |
| title_full_unstemmed | Ruminal bacterial diversity of yaks (Bos grunniens) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries |
| title_short | Ruminal bacterial diversity of yaks (Bos grunniens) fed by grazing or indoor regime on the Tibetan Plateau by analysis of 16S rRNA gene libraries |
| title_sort | ruminal bacterial diversity of yaks bos grunniens fed by grazing or indoor regime on the tibetan plateau by analysis of 16s rrna gene libraries |
| topic | Grazing yak Indoor-feeding yak Rumen bacteria diversity 16S rRNA gene |
| url | http://www.aspajournal.it/index.php/ijas/article/view/3970 |
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