RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUS

Abstract: Pituitary transcription factor Pit-1, which belongs to a large POU domain family is a positive regulatory factor of growth hormone, prolactin and thyrotropin β-subunit in the mammalian pituitary. Therefore, the gene encoding Pit-1 was chosen as a candidate gene to investigate its associati...

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Main Authors: VIORICA COŞIER, A. VLAIC, CRINA CARŞAI, CLAUDIA SOCOL, R. CONSTANTINESCU
Format: Article
Language:English
Published: Agroprint Timisoara 2023-11-01
Series:Scientific Papers Animal Science and Biotechnologies
Subjects:
Online Access:https://spasb.ro/index.php/public_html/article/view/1676
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author VIORICA COŞIER
A. VLAIC
CRINA CARŞAI
CLAUDIA SOCOL
R. CONSTANTINESCU
author_facet VIORICA COŞIER
A. VLAIC
CRINA CARŞAI
CLAUDIA SOCOL
R. CONSTANTINESCU
author_sort VIORICA COŞIER
collection DOAJ
description Abstract: Pituitary transcription factor Pit-1, which belongs to a large POU domain family is a positive regulatory factor of growth hormone, prolactin and thyrotropin β-subunit in the mammalian pituitary. Therefore, the gene encoding Pit-1 was chosen as a candidate gene to investigate its association with lactation performance in cattle. The present study was carried out to establish the genetic structure at this locus in two Romanian cattle breeds: Romanian Simmental and Maramures Brown, to establish the possible association between genotype and milk yield and conformation traits. A strategy employing polymerase chain reaction was used to amplify a 1355- pb fragment from blood DNA and digestion with HinfI enzyme and the genetic structure was estimated for both breeds.
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spelling doaj.art-9be1b462b91b4f50a3635c1694b354762024-04-09T08:53:18ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-0141145451676RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUSVIORICA COŞIER0A. VLAIC1CRINA CARŞAI2CLAUDIA SOCOL3R. CONSTANTINESCU4University of Agriculture Sciences and Veterinary Medicine of Cluj – Napoca - Faculty of Animal Sciences and BiotechnologyUniversity of Agriculture Sciences and Veterinary Medicine of Cluj – Napoca - Faculty of Animal Sciences and BiotechnologyUniversity of Agriculture Sciences and Veterinary Medicine of Cluj – Napoca - Faculty of Animal Sciences and BiotechnologyUniversity of Agriculture Sciences and Veterinary Medicine of Cluj – Napoca - Faculty of Animal Sciences and Biotechnology University of Agriculture Sciences and Veterinary Medicine of Cluj – Napoca - Faculty of Animal Sciences and BiotechnologyAbstract: Pituitary transcription factor Pit-1, which belongs to a large POU domain family is a positive regulatory factor of growth hormone, prolactin and thyrotropin β-subunit in the mammalian pituitary. Therefore, the gene encoding Pit-1 was chosen as a candidate gene to investigate its association with lactation performance in cattle. The present study was carried out to establish the genetic structure at this locus in two Romanian cattle breeds: Romanian Simmental and Maramures Brown, to establish the possible association between genotype and milk yield and conformation traits. A strategy employing polymerase chain reaction was used to amplify a 1355- pb fragment from blood DNA and digestion with HinfI enzyme and the genetic structure was estimated for both breeds.https://spasb.ro/index.php/public_html/article/view/1676polymorphismgenetranscription factorgenetic structurepcrhinfi
spellingShingle VIORICA COŞIER
A. VLAIC
CRINA CARŞAI
CLAUDIA SOCOL
R. CONSTANTINESCU
RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUS
Scientific Papers Animal Science and Biotechnologies
polymorphism
gene
transcription factor
genetic structure
pcr
hinfi
title RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUS
title_full RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUS
title_fullStr RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUS
title_full_unstemmed RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUS
title_short RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUS
title_sort research concerning the genetic structure of romanian simental and maramures brown breeeds at the pituitary transcription factor locus
topic polymorphism
gene
transcription factor
genetic structure
pcr
hinfi
url https://spasb.ro/index.php/public_html/article/view/1676
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