Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid
Natural free amino acids present in plant extracts or tea infusions provide a unique flavor and potential effect on anxiety and blood pressure reduction. Accordingly, quantifying free amino acids in foods has been of interest to food science and analytical research fields. The ninhydrin solution-bas...
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/1/49 |
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| author | Suhui Jeong Yeji Jeon Jaehun Mun Se Min Jeong Huiling Liang Kyeongwoon Chung Pyong-In Yi Beum-Soo An Sungbaek Seo |
| author_facet | Suhui Jeong Yeji Jeon Jaehun Mun Se Min Jeong Huiling Liang Kyeongwoon Chung Pyong-In Yi Beum-Soo An Sungbaek Seo |
| author_sort | Suhui Jeong |
| collection | DOAJ |
| description | Natural free amino acids present in plant extracts or tea infusions provide a unique flavor and potential effect on anxiety and blood pressure reduction. Accordingly, quantifying free amino acids in foods has been of interest to food science and analytical research fields. The ninhydrin solution-based assay is a colorimetric method based on the formation and detection of Ruhemann’s purple complex. Media-based colorimetric detection requires specialized facilities and personnel; moreover, it can suffer from the interference of the analyte color. In this study, we developed ninhydrin-loaded microcapsules and a simple free amino acids detection procedure, by simply dipping the microcapsules into the analyte solution for 3 min. Among the five tested natural free amino acids, theanine exhibited the highest colorimetric response to microcapsule-based detection, with a limit of detection of 0.826 mM. |
| first_indexed | 2024-03-09T13:10:47Z |
| format | Article |
| id | doaj.art-9be4dab748b84151942ad6c3e327c2f3 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T13:10:47Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-9be4dab748b84151942ad6c3e327c2f32023-11-30T21:41:53ZengMDPI AGChemosensors2227-90402023-01-011114910.3390/chemosensors11010049Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino AcidSuhui Jeong0Yeji Jeon1Jaehun Mun2Se Min Jeong3Huiling Liang4Kyeongwoon Chung5Pyong-In Yi6Beum-Soo An7Sungbaek Seo8Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of KoreaTea Research Institute, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, ChinaDepartment of Biofibers and Biomaterials Science, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of KoreaNatural free amino acids present in plant extracts or tea infusions provide a unique flavor and potential effect on anxiety and blood pressure reduction. Accordingly, quantifying free amino acids in foods has been of interest to food science and analytical research fields. The ninhydrin solution-based assay is a colorimetric method based on the formation and detection of Ruhemann’s purple complex. Media-based colorimetric detection requires specialized facilities and personnel; moreover, it can suffer from the interference of the analyte color. In this study, we developed ninhydrin-loaded microcapsules and a simple free amino acids detection procedure, by simply dipping the microcapsules into the analyte solution for 3 min. Among the five tested natural free amino acids, theanine exhibited the highest colorimetric response to microcapsule-based detection, with a limit of detection of 0.826 mM.https://www.mdpi.com/2227-9040/11/1/49microcapsulesnatural free amino acidninhydrintheanine |
| spellingShingle | Suhui Jeong Yeji Jeon Jaehun Mun Se Min Jeong Huiling Liang Kyeongwoon Chung Pyong-In Yi Beum-Soo An Sungbaek Seo Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid Chemosensors microcapsules natural free amino acid ninhydrin theanine |
| title | Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid |
| title_full | Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid |
| title_fullStr | Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid |
| title_full_unstemmed | Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid |
| title_short | Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid |
| title_sort | ninhydrin loaded microcapsules for detection of natural free amino acid |
| topic | microcapsules natural free amino acid ninhydrin theanine |
| url | https://www.mdpi.com/2227-9040/11/1/49 |
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