Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in <i>Arabidopsis</i> Roots
The remodeling of root architecture is regarded as a major development to improve the plant’s adaptivity to phosphate (Pi)-deficient conditions. The WRKY transcription factors family has been reported to regulate the Pi-deficiency-induced systemic responses by affecting Pi absorption or transportati...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/17/9275 |
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| author | Nuo Shen Sifan Hou Guoqing Tu Wenzhi Lan Yanping Jing |
| author_facet | Nuo Shen Sifan Hou Guoqing Tu Wenzhi Lan Yanping Jing |
| author_sort | Nuo Shen |
| collection | DOAJ |
| description | The remodeling of root architecture is regarded as a major development to improve the plant’s adaptivity to phosphate (Pi)-deficient conditions. The WRKY transcription factors family has been reported to regulate the Pi-deficiency-induced systemic responses by affecting Pi absorption or transportation. Whether these transcription factors act as a regulator to mediate the Pi-deficiency-induced remodeling of root architecture, a typical local response, is still unclear. Here, we identified an <i>Arabidopsis</i> transcription factor, WRKY33, that acted as a negative regulator to mediate the Pi-deficiency-induced remodeling of root architecture. The disruption of WRKY33 in wrky33-2 mutant increased the plant’s low Pi sensitivity by further inhibiting the primary root growth and promoting the formation of root hair. Furthermore, we revealed that WRKY33 negatively regulated the remodeling of root architecture by controlling the transcriptional expression of ALMT1 under Pi-deficient conditions, which further mediated the Fe<sup>3+</sup> accumulation in root tips to inhibit the root growth. In conclusion, this study demonstrates a previously unrecognized signaling crosstalk between WRKY33 and the ALMT1-mediated malate transport system to regulate the Pi deficiency responses. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:11:53Z |
| publishDate | 2021-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9be7149bdc874afca4b1dbed4226fa2d2023-11-22T10:40:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012217927510.3390/ijms22179275Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in <i>Arabidopsis</i> RootsNuo Shen0Sifan Hou1Guoqing Tu2Wenzhi Lan3Yanping Jing4State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, ChinaState Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, ChinaState Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, ChinaState Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaThe remodeling of root architecture is regarded as a major development to improve the plant’s adaptivity to phosphate (Pi)-deficient conditions. The WRKY transcription factors family has been reported to regulate the Pi-deficiency-induced systemic responses by affecting Pi absorption or transportation. Whether these transcription factors act as a regulator to mediate the Pi-deficiency-induced remodeling of root architecture, a typical local response, is still unclear. Here, we identified an <i>Arabidopsis</i> transcription factor, WRKY33, that acted as a negative regulator to mediate the Pi-deficiency-induced remodeling of root architecture. The disruption of WRKY33 in wrky33-2 mutant increased the plant’s low Pi sensitivity by further inhibiting the primary root growth and promoting the formation of root hair. Furthermore, we revealed that WRKY33 negatively regulated the remodeling of root architecture by controlling the transcriptional expression of ALMT1 under Pi-deficient conditions, which further mediated the Fe<sup>3+</sup> accumulation in root tips to inhibit the root growth. In conclusion, this study demonstrates a previously unrecognized signaling crosstalk between WRKY33 and the ALMT1-mediated malate transport system to regulate the Pi deficiency responses.https://www.mdpi.com/1422-0067/22/17/9275phosphate deficiencyroot architectureiron homeostasistranscription factor |
| spellingShingle | Nuo Shen Sifan Hou Guoqing Tu Wenzhi Lan Yanping Jing Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in <i>Arabidopsis</i> Roots International Journal of Molecular Sciences phosphate deficiency root architecture iron homeostasis transcription factor |
| title | Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in <i>Arabidopsis</i> Roots |
| title_full | Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in <i>Arabidopsis</i> Roots |
| title_fullStr | Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in <i>Arabidopsis</i> Roots |
| title_full_unstemmed | Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in <i>Arabidopsis</i> Roots |
| title_short | Transcription Factor WRKY33 Mediates the Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in <i>Arabidopsis</i> Roots |
| title_sort | transcription factor wrky33 mediates the phosphate deficiency induced remodeling of root architecture by modulating iron homeostasis in i arabidopsis i roots |
| topic | phosphate deficiency root architecture iron homeostasis transcription factor |
| url | https://www.mdpi.com/1422-0067/22/17/9275 |
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